Advanced

Identifying bacteria using DNA binding maps

Emilsson, Gustav; Nilsson, Adam LU ; Nyberg, Lena K. LU ; Noble, Charleston LU ; Svensson Stadler, Liselott; Moore, Edward R.B.; Ambjörnsson, Tobias LU ; Tegenfeldt, Jonas O. LU and Westerlund, Fredrik (2013) 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 1. p.473-475
Abstract

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces... (More)

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Bacterial identification, Nanofluidics, Optical mapping
host publication
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
volume
1
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
conference location
Freiburg, Germany
conference dates
2013-10-27 - 2013-10-31
external identifiers
  • scopus:84907335679
ISBN
9781632666246
language
English
LU publication?
yes
id
05f18cd8-f5c0-45d0-bc9e-83a96d76a15f
date added to LUP
2018-10-20 08:40:25
date last changed
2019-02-20 11:32:14
@inproceedings{05f18cd8-f5c0-45d0-bc9e-83a96d76a15f,
  abstract     = {<p>We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.</p>},
  author       = {Emilsson, Gustav and Nilsson, Adam and Nyberg, Lena K. and Noble, Charleston and Svensson Stadler, Liselott and Moore, Edward R.B. and Ambjörnsson, Tobias and Tegenfeldt, Jonas O. and Westerlund, Fredrik},
  isbn         = {9781632666246},
  keyword      = {Bacterial identification,Nanofluidics,Optical mapping},
  language     = {eng},
  location     = {Freiburg, Germany},
  month        = {01},
  pages        = {473--475},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Identifying bacteria using DNA binding maps},
  volume       = {1},
  year         = {2013},
}