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Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics

Cardellini, Jacopo ; Ridolfi, Andrea ; Donati, Melissa ; Giampietro, Valentina ; Severi, Mirko ; Brucale, Marco ; Valle, Francesco ; Bergese, Paolo ; Montis, Costanza and Caselli, Lucrezia LU , et al. (2023) In Journal of Colloid and Interface Science 640. p.100-109
Abstract

Although promising for biomedicine, the clinical translation of inorganic nanoparticles (NPs) is limited by low biocompatibility and stability in biological fluids. A common strategy to circumvent this drawback consists in disguising the active inorganic core with a lipid bilayer coating, reminiscent of the structure of the cell membrane to redefine the chemical and biological identity of NPs. While recent reports introduced membrane-coating procedures for NPs, a robust and accessible method to quantify the integrity of the bilayer coverage is not yet available. To fill this gap, we prepared SiO2 nanoparticles (SiO2NPs) with different membrane coverage degrees and monitored their interaction with AuNPs by combining... (More)

Although promising for biomedicine, the clinical translation of inorganic nanoparticles (NPs) is limited by low biocompatibility and stability in biological fluids. A common strategy to circumvent this drawback consists in disguising the active inorganic core with a lipid bilayer coating, reminiscent of the structure of the cell membrane to redefine the chemical and biological identity of NPs. While recent reports introduced membrane-coating procedures for NPs, a robust and accessible method to quantify the integrity of the bilayer coverage is not yet available. To fill this gap, we prepared SiO2 nanoparticles (SiO2NPs) with different membrane coverage degrees and monitored their interaction with AuNPs by combining microscopic, scattering, and optical techniques. The membrane-coating on SiO2NPs induces spontaneous clustering of AuNPs, whose extent depends on the coating integrity. Remarkably, we discovered a linear correlation between the membrane coverage and a spectral descriptor for the AuNPs’ plasmonic resonance, spanning a wide range of coating yields. These results provide a fast and cost-effective assay to monitor the compatibilization of NPs with biological environments, essential for bench tests and scale-up. In addition, we introduce a robust and scalable method to prepare SiO2NPs/AuNPs hybrids through spontaneous self-assembly, with a high-fidelity structural control mediated by a lipid bilayer.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomimetic nanoparticles, Extracellular vesicles, Gold nanoparticles, Membrane-coated nanoparticles, Nano-bio interface, Nanomedicine, Nanoplasmonics, Silica nanoparticles
in
Journal of Colloid and Interface Science
volume
640
pages
10 pages
publisher
Elsevier
external identifiers
  • pmid:36842416
  • scopus:85149057277
ISSN
0021-9797
DOI
10.1016/j.jcis.2023.02.073
language
English
LU publication?
yes
id
060d1e59-fe42-4a98-ab7f-cc458b20b6e5
date added to LUP
2023-03-13 13:34:19
date last changed
2024-06-15 03:17:20
@article{060d1e59-fe42-4a98-ab7f-cc458b20b6e5,
  abstract     = {{<p>Although promising for biomedicine, the clinical translation of inorganic nanoparticles (NPs) is limited by low biocompatibility and stability in biological fluids. A common strategy to circumvent this drawback consists in disguising the active inorganic core with a lipid bilayer coating, reminiscent of the structure of the cell membrane to redefine the chemical and biological identity of NPs. While recent reports introduced membrane-coating procedures for NPs, a robust and accessible method to quantify the integrity of the bilayer coverage is not yet available. To fill this gap, we prepared SiO<sub>2</sub> nanoparticles (SiO<sub>2</sub>NPs) with different membrane coverage degrees and monitored their interaction with AuNPs by combining microscopic, scattering, and optical techniques. The membrane-coating on SiO<sub>2</sub>NPs induces spontaneous clustering of AuNPs, whose extent depends on the coating integrity. Remarkably, we discovered a linear correlation between the membrane coverage and a spectral descriptor for the AuNPs’ plasmonic resonance, spanning a wide range of coating yields. These results provide a fast and cost-effective assay to monitor the compatibilization of NPs with biological environments, essential for bench tests and scale-up. In addition, we introduce a robust and scalable method to prepare SiO<sub>2</sub>NPs/AuNPs hybrids through spontaneous self-assembly, with a high-fidelity structural control mediated by a lipid bilayer.</p>}},
  author       = {{Cardellini, Jacopo and Ridolfi, Andrea and Donati, Melissa and Giampietro, Valentina and Severi, Mirko and Brucale, Marco and Valle, Francesco and Bergese, Paolo and Montis, Costanza and Caselli, Lucrezia and Berti, Debora}},
  issn         = {{0021-9797}},
  keywords     = {{Biomimetic nanoparticles; Extracellular vesicles; Gold nanoparticles; Membrane-coated nanoparticles; Nano-bio interface; Nanomedicine; Nanoplasmonics; Silica nanoparticles}},
  language     = {{eng}},
  month        = {{06}},
  pages        = {{100--109}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Colloid and Interface Science}},
  title        = {{Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics}},
  url          = {{http://dx.doi.org/10.1016/j.jcis.2023.02.073}},
  doi          = {{10.1016/j.jcis.2023.02.073}},
  volume       = {{640}},
  year         = {{2023}},
}