Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Multi-scale characterization of the spatio-temporal interplay between elemental composition, mineral deposition and remodelling in bone fracture healing

Dejea, Hector LU ; Raina, Deepak LU ; Silva Barreto, Isabella LU orcid ; Sharma, Kunal LU ; Liu, Yang LU ; Ferreira Sanchez, Dario ; Johansson, Ulf LU and Isaksson, Hanna LU orcid (2023) In Acta Biomaterialia 167. p.135-146
Abstract
Bone mineralization involves a complex orchestration of physico-chemical responses from the organism. Despite extensive studies, the detailed mechanisms of mineralization remain to be elucidated. This study aims to characterize bone mineralization using an in-vivo long bone fracture healing model in the rat. The spatio-temporal distribution of relevant elements was correlated to the deposition and maturation of hydroxyapatite and the presence of matrix remodeling compounds (MMP-13). Multi-scale measurements indicated that (i) zinc is required for both the initial mineral deposition and resorption processes during mature mineral remodeling; (ii) Zinc and MMP-13 show similar spatio-temporal trends during early mineralization; (iii) Iron acts... (More)
Bone mineralization involves a complex orchestration of physico-chemical responses from the organism. Despite extensive studies, the detailed mechanisms of mineralization remain to be elucidated. This study aims to characterize bone mineralization using an in-vivo long bone fracture healing model in the rat. The spatio-temporal distribution of relevant elements was correlated to the deposition and maturation of hydroxyapatite and the presence of matrix remodeling compounds (MMP-13). Multi-scale measurements indicated that (i) zinc is required for both the initial mineral deposition and resorption processes during mature mineral remodeling; (ii) Zinc and MMP-13 show similar spatio-temporal trends during early mineralization; (iii) Iron acts locally and in coordination with zinc during mineralization, thus indicating novel evidence of the time-events and inter-play between the elements. These findings improve the understanding of bone mineralization by explaining the link between the different constituents of this process throughout the healing time. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bone mineralization, Endochondral ossification, Fracture healing, Multi-scale, Multi-modal
in
Acta Biomaterialia
volume
167
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:85165661495
ISSN
1742-7061
DOI
10.1016/j.actbio.2023.06.031
language
English
LU publication?
yes
id
06a2aa20-5664-4f40-b53f-d21c67d88a3a
date added to LUP
2023-10-02 11:58:09
date last changed
2023-11-21 23:12:45
@article{06a2aa20-5664-4f40-b53f-d21c67d88a3a,
  abstract     = {{Bone mineralization involves a complex orchestration of physico-chemical responses from the organism. Despite extensive studies, the detailed mechanisms of mineralization remain to be elucidated. This study aims to characterize bone mineralization using an in-vivo long bone fracture healing model in the rat. The spatio-temporal distribution of relevant elements was correlated to the deposition and maturation of hydroxyapatite and the presence of matrix remodeling compounds (MMP-13). Multi-scale measurements indicated that (i) zinc is required for both the initial mineral deposition and resorption processes during mature mineral remodeling; (ii) Zinc and MMP-13 show similar spatio-temporal trends during early mineralization; (iii) Iron acts locally and in coordination with zinc during mineralization, thus indicating novel evidence of the time-events and inter-play between the elements. These findings improve the understanding of bone mineralization by explaining the link between the different constituents of this process throughout the healing time.}},
  author       = {{Dejea, Hector and Raina, Deepak and Silva Barreto, Isabella and Sharma, Kunal and Liu, Yang and Ferreira Sanchez, Dario and Johansson, Ulf and Isaksson, Hanna}},
  issn         = {{1742-7061}},
  keywords     = {{Bone mineralization; Endochondral ossification; Fracture healing; Multi-scale; Multi-modal}},
  language     = {{eng}},
  month        = {{09}},
  pages        = {{135--146}},
  publisher    = {{Elsevier}},
  series       = {{Acta Biomaterialia}},
  title        = {{Multi-scale characterization of the spatio-temporal interplay between elemental composition, mineral deposition and remodelling in bone fracture healing}},
  url          = {{http://dx.doi.org/10.1016/j.actbio.2023.06.031}},
  doi          = {{10.1016/j.actbio.2023.06.031}},
  volume       = {{167}},
  year         = {{2023}},
}