Megahertz serial crystallography
(2018) In Nature Communications 9(1). p.4025-4025- Abstract
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial... (More)
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
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- author
- author collaboration
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- 2018
- type
- Contribution to journal
- publication status
- published
- in
- Nature Communications
- volume
- 9
- issue
- 1
- pages
- 1 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:30279492
- scopus:85054197272
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-018-06156-7
- language
- English
- LU publication?
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- id
- 06b600bd-297d-483f-8b29-0e23d9b8f083
- date added to LUP
- 2019-03-29 16:54:19
- date last changed
- 2024-06-12 10:11:58
@article{06b600bd-297d-483f-8b29-0e23d9b8f083,
abstract = {{<p>The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.</p>}},
author = {{Wiedorn, Max O and Oberthür, Dominik and Bean, Richard and Schubert, Robin and Werner, Nadine and Abbey, Brian and Aepfelbacher, Martin and Adriano, Luigi and Allahgholi, Aschkan and Al-Qudami, Nasser and Andreasson, Jakob and Aplin, Steve and Awel, Salah and Ayyer, Kartik and Bajt, Saša and Barák, Imrich and Bari, Sadia and Bielecki, Johan and Botha, Sabine and Boukhelef, Djelloul and Brehm, Wolfgang and Brockhauser, Sandor and Cheviakov, Igor and Coleman, Matthew A and Cruz-Mazo, Francisco and Danilevski, Cyril and Darmanin, Connie and Doak, R Bruce and Domaracky, Martin and Dörner, Katerina and Du, Yang and Fangohr, Hans and Fleckenstein, Holger and Frank, Matthias and Fromme, Petra and Gañán-Calvo, Alfonso M and Gevorkov, Yaroslav and Giewekemeyer, Klaus and Ginn, Helen Mary and Graafsma, Heinz and Graceffa, Rita and Greiffenberg, Dominic and Gumprecht, Lars and Göttlicher, Peter and Hajdu, Janos and Hauf, Steffen and Heymann, Michael and Holmes, Susannah and Horke, Daniel A and Schulz, Joachim}},
issn = {{2041-1723}},
language = {{eng}},
number = {{1}},
pages = {{4025--4025}},
publisher = {{Nature Publishing Group}},
series = {{Nature Communications}},
title = {{Megahertz serial crystallography}},
url = {{http://dx.doi.org/10.1038/s41467-018-06156-7}},
doi = {{10.1038/s41467-018-06156-7}},
volume = {{9}},
year = {{2018}},
}