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Megahertz serial crystallography

Wiedorn, Max O ; Oberthür, Dominik ; Bean, Richard ; Schubert, Robin ; Werner, Nadine ; Abbey, Brian ; Aepfelbacher, Martin ; Adriano, Luigi ; Allahgholi, Aschkan and Al-Qudami, Nasser , et al. (2018) In Nature Communications 9(1). p.4025-4025
Abstract

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial... (More)

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

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author collaboration
publishing date
type
Contribution to journal
publication status
published
in
Nature Communications
volume
9
issue
1
pages
1 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:85054197272
  • pmid:30279492
ISSN
2041-1723
DOI
10.1038/s41467-018-06156-7
language
English
LU publication?
no
id
06b600bd-297d-483f-8b29-0e23d9b8f083
date added to LUP
2019-03-29 16:54:19
date last changed
2020-12-01 03:20:38
@article{06b600bd-297d-483f-8b29-0e23d9b8f083,
  abstract     = {<p>The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.</p>},
  author       = {Wiedorn, Max O and Oberthür, Dominik and Bean, Richard and Schubert, Robin and Werner, Nadine and Abbey, Brian and Aepfelbacher, Martin and Adriano, Luigi and Allahgholi, Aschkan and Al-Qudami, Nasser and Andreasson, Jakob and Aplin, Steve and Awel, Salah and Ayyer, Kartik and Bajt, Saša and Barák, Imrich and Bari, Sadia and Bielecki, Johan and Botha, Sabine and Boukhelef, Djelloul and Brehm, Wolfgang and Brockhauser, Sandor and Cheviakov, Igor and Coleman, Matthew A and Cruz-Mazo, Francisco and Danilevski, Cyril and Darmanin, Connie and Doak, R Bruce and Domaracky, Martin and Dörner, Katerina and Du, Yang and Fangohr, Hans and Fleckenstein, Holger and Frank, Matthias and Fromme, Petra and Gañán-Calvo, Alfonso M and Gevorkov, Yaroslav and Giewekemeyer, Klaus and Ginn, Helen Mary and Graafsma, Heinz and Graceffa, Rita and Greiffenberg, Dominic and Gumprecht, Lars and Göttlicher, Peter and Hajdu, Janos and Hauf, Steffen and Heymann, Michael and Holmes, Susannah and Horke, Daniel A and Schulz, Joachim},
  issn         = {2041-1723},
  language     = {eng},
  number       = {1},
  pages        = {4025--4025},
  publisher    = {Nature Publishing Group},
  series       = {Nature Communications},
  title        = {Megahertz serial crystallography},
  url          = {http://dx.doi.org/10.1038/s41467-018-06156-7},
  doi          = {10.1038/s41467-018-06156-7},
  volume       = {9},
  year         = {2018},
}