Identification of pili on the surface of Finegoldia magna - A Gram-positive anaerobic cocci.
(2014) In Anaerobe 27(Mar 29). p.40-49- Abstract
- Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint... (More)
- Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint infections. In this study, F. magna ALB8 was found to harbour three sortases at the pilus locus, two of which bear high similarity to class C sortases in Streptococcus pneumoniae. Two putative sortase-dependent pili proteins were found in the locus, with one being identified as the major pilus subunit, Fmp1 (F. magna pilus subunit 1), due to its high similarity to other major pilus proteins in prominent Gram-positive pathogens. The presence of sortase-dependent pili was confirmed experimentally through recombinant production of Fmp1 and production of antiserum. The Fmp1 antiserum was used in Western blot to show the presence of a high molecular weight protein ladder, characteristic of the presence of pili, in trypsin released cell wall surface proteins from F. magna. The presence of sortase-dependent pili was visually confirmed by transmission electron microscopy, which showed the binding of gold labelled anti-Fmp1 to individual pilus proteins along the pilus. Furthermore, pili could also be found to bind and interact with keratinocytes in the epidermal layer of human skin, suggesting an adhesive role for pili on F. magna. Our work represents the first description of pilus structures in F. magna. This discovery further elucidates F. magna physiology and allows for additional analysis of host-bacterial interactions in future studies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4431388
- author
- Murphy, Elizabeth LU ; Janulczyk, Robert LU ; Karlsson, Christofer LU ; Mörgelin, Matthias LU and Frick, Inga-Maria LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Anaerobe
- volume
- 27
- issue
- Mar 29
- pages
- 40 - 49
- publisher
- Elsevier
- external identifiers
-
- pmid:24685556
- wos:000337659600009
- scopus:84898623406
- pmid:24685556
- ISSN
- 1095-8274
- DOI
- 10.1016/j.anaerobe.2014.03.008
- language
- English
- LU publication?
- yes
- id
- 070e646c-8072-4721-a079-d1abf1ab9b51 (old id 4431388)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24685556?dopt=Abstract
- date added to LUP
- 2016-04-01 10:32:03
- date last changed
- 2022-02-10 03:02:14
@article{070e646c-8072-4721-a079-d1abf1ab9b51,
abstract = {{Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint infections. In this study, F. magna ALB8 was found to harbour three sortases at the pilus locus, two of which bear high similarity to class C sortases in Streptococcus pneumoniae. Two putative sortase-dependent pili proteins were found in the locus, with one being identified as the major pilus subunit, Fmp1 (F. magna pilus subunit 1), due to its high similarity to other major pilus proteins in prominent Gram-positive pathogens. The presence of sortase-dependent pili was confirmed experimentally through recombinant production of Fmp1 and production of antiserum. The Fmp1 antiserum was used in Western blot to show the presence of a high molecular weight protein ladder, characteristic of the presence of pili, in trypsin released cell wall surface proteins from F. magna. The presence of sortase-dependent pili was visually confirmed by transmission electron microscopy, which showed the binding of gold labelled anti-Fmp1 to individual pilus proteins along the pilus. Furthermore, pili could also be found to bind and interact with keratinocytes in the epidermal layer of human skin, suggesting an adhesive role for pili on F. magna. Our work represents the first description of pilus structures in F. magna. This discovery further elucidates F. magna physiology and allows for additional analysis of host-bacterial interactions in future studies.}},
author = {{Murphy, Elizabeth and Janulczyk, Robert and Karlsson, Christofer and Mörgelin, Matthias and Frick, Inga-Maria}},
issn = {{1095-8274}},
language = {{eng}},
number = {{Mar 29}},
pages = {{40--49}},
publisher = {{Elsevier}},
series = {{Anaerobe}},
title = {{Identification of pili on the surface of Finegoldia magna - A Gram-positive anaerobic cocci.}},
url = {{https://lup.lub.lu.se/search/files/1925274/4762167}},
doi = {{10.1016/j.anaerobe.2014.03.008}},
volume = {{27}},
year = {{2014}},
}