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In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations

Safi, Fatemeh LU ; Dhapola, Parashar LU ; Erlandsson, Eva LU ; Ulfsson, Linda Geironson LU ; Calderón, Ariana S. LU ; Böiers, Charlotta LU and Karlsson, Göran LU (2023) In STAR Protocols 4(1).
Abstract

Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).1

Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bioinformatics, Cell Biology, Cell culture, Cell isolation, Flow Cytometry/Mass Cytometry, Genomics, Molecular Biology, Stem Cells
in
STAR Protocols
volume
4
issue
1
article number
101965
publisher
Cell Press
external identifiers
  • scopus:85146080918
  • pmid:36633951
ISSN
2666-1667
DOI
10.1016/j.xpro.2022.101965
language
English
LU publication?
yes
id
07404e56-bfba-44a9-acf9-8317e259902d
date added to LUP
2023-02-16 15:20:28
date last changed
2024-06-23 10:44:52
@article{07404e56-bfba-44a9-acf9-8317e259902d,
  abstract     = {{<p>Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).<sup>1</sup></p>}},
  author       = {{Safi, Fatemeh and Dhapola, Parashar and Erlandsson, Eva and Ulfsson, Linda Geironson and Calderón, Ariana S. and Böiers, Charlotta and Karlsson, Göran}},
  issn         = {{2666-1667}},
  keywords     = {{Bioinformatics; Cell Biology; Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Genomics; Molecular Biology; Stem Cells}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{1}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2022.101965}},
  doi          = {{10.1016/j.xpro.2022.101965}},
  volume       = {{4}},
  year         = {{2023}},
}