In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations
(2023) In STAR Protocols 4(1).- Abstract
Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).1
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/07404e56-bfba-44a9-acf9-8317e259902d
- author
- Safi, Fatemeh LU ; Dhapola, Parashar LU ; Erlandsson, Eva LU ; Ulfsson, Linda Geironson LU ; Calderón, Ariana S. LU ; Böiers, Charlotta LU and Karlsson, Göran LU
- organization
- publishing date
- 2023-03-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bioinformatics, Cell Biology, Cell culture, Cell isolation, Flow Cytometry/Mass Cytometry, Genomics, Molecular Biology, Stem Cells
- in
- STAR Protocols
- volume
- 4
- issue
- 1
- article number
- 101965
- publisher
- Cell Press
- external identifiers
-
- pmid:36633951
- scopus:85146080918
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2022.101965
- language
- English
- LU publication?
- yes
- id
- 07404e56-bfba-44a9-acf9-8317e259902d
- date added to LUP
- 2023-02-16 15:20:28
- date last changed
- 2024-09-15 19:05:24
@article{07404e56-bfba-44a9-acf9-8317e259902d, abstract = {{<p>Here we describe an in vitro co-culture system that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at clonal level. We present both a sensitive single-cell switch-culture system as well as a less laborious alternative barcoding protocol more convenient for larger cell numbers. Importantly, generation of all lineages from single long-term hematopoietic stem cells are described, following 21 days of culture. This protocol represents an efficient tool for validation experiments for single-cell genomics data. For complete details on the use and execution of this protocol, please refer to Safi et al. (2022).<sup>1</sup></p>}}, author = {{Safi, Fatemeh and Dhapola, Parashar and Erlandsson, Eva and Ulfsson, Linda Geironson and Calderón, Ariana S. and Böiers, Charlotta and Karlsson, Göran}}, issn = {{2666-1667}}, keywords = {{Bioinformatics; Cell Biology; Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Genomics; Molecular Biology; Stem Cells}}, language = {{eng}}, month = {{03}}, number = {{1}}, publisher = {{Cell Press}}, series = {{STAR Protocols}}, title = {{In vitro clonal multilineage differentiation of distinct murine hematopoietic progenitor populations}}, url = {{http://dx.doi.org/10.1016/j.xpro.2022.101965}}, doi = {{10.1016/j.xpro.2022.101965}}, volume = {{4}}, year = {{2023}}, }