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Generalized olfactory detection of floral volatiles in the highly specialized Greya-Lithophragma nursery pollination system

Schiestl, Florian P. ; Wallin, Erika A. ; Beck, John J. ; Friberg, Magne LU and Thompson, John N. (2021) In Arthropod-Plant Interactions 15. p.209-221
Abstract

Volatiles are of key importance for host-plant recognition in insects. In the pollination system of Lithophragma flowers and Greya moths, moths are highly specialized on Lithophragma, in which they oviposit and thereby pollinate the flowers. Floral volatiles in Lithophragma are highly variable between species and populations, and moths prefer to oviposit into Lithophragma flowers from populations of the local host species. Here we used gas chromatography coupled with electroantennographic detection (GC-EAD) to test whether Greya moths detect specific key volatiles or respond broadly to many volatiles of Lithophragma flowers. We also addressed whether olfactory detection in Greya moths varies across populations, consistent with a... (More)

Volatiles are of key importance for host-plant recognition in insects. In the pollination system of Lithophragma flowers and Greya moths, moths are highly specialized on Lithophragma, in which they oviposit and thereby pollinate the flowers. Floral volatiles in Lithophragma are highly variable between species and populations, and moths prefer to oviposit into Lithophragma flowers from populations of the local host species. Here we used gas chromatography coupled with electroantennographic detection (GC-EAD) to test whether Greya moths detect specific key volatiles or respond broadly to many volatiles of Lithophragma flowers. We also addressed whether olfactory detection in Greya moths varies across populations, consistent with a co-evolutionary scenario. We analyzed flower volatile samples from three different species and five populations of Lithophragma occurring across a 1400 km range in the Western USA, and their sympatric female Greya politella moths. We showed that Greya politella detect a broad range of Lithophragma volatiles, with a total of 23 compounds being EAD active. We chemically identified 15 of these, including the chiral 6, 10, 14-trimethylpentadecan-2-one (hexahydrofarnesyl acetone), which was not previously detected in Lithophragma. All investigated Lithophragma species produced the (6R, 10R)-enantiomer of this compound. We showed that Greya moths detected not only volatiles of their local Lithophragma plants, but also those from allopatric populations/species that they not encounter in local populations. In conclusion, the generalized detection of volatiles and a lack of co-divergence between volatiles and olfactory detection may be of selective advantage for moths in tracking hosts with rapidly evolving, chemically diverse floral volatiles.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Coevolution, Floral scent, GC-EAD, Olfaction, Olfactory receptors, Pollination
in
Arthropod-Plant Interactions
volume
15
pages
209 - 221
publisher
Springer
external identifiers
  • scopus:85102051348
ISSN
1872-8855
DOI
10.1007/s11829-021-09809-5
language
English
LU publication?
yes
id
07417ac6-fd21-47fa-a931-d0c2afcc6b0e
date added to LUP
2021-03-22 10:18:39
date last changed
2021-03-22 10:18:39
@article{07417ac6-fd21-47fa-a931-d0c2afcc6b0e,
  abstract     = {<p>Volatiles are of key importance for host-plant recognition in insects. In the pollination system of Lithophragma flowers and Greya moths, moths are highly specialized on Lithophragma, in which they oviposit and thereby pollinate the flowers. Floral volatiles in Lithophragma are highly variable between species and populations, and moths prefer to oviposit into Lithophragma flowers from populations of the local host species. Here we used gas chromatography coupled with electroantennographic detection (GC-EAD) to test whether Greya moths detect specific key volatiles or respond broadly to many volatiles of Lithophragma flowers. We also addressed whether olfactory detection in Greya moths varies across populations, consistent with a co-evolutionary scenario. We analyzed flower volatile samples from three different species and five populations of Lithophragma occurring across a 1400 km range in the Western USA, and their sympatric female Greya politella moths. We showed that Greya politella detect a broad range of Lithophragma volatiles, with a total of 23 compounds being EAD active. We chemically identified 15 of these, including the chiral 6, 10, 14-trimethylpentadecan-2-one (hexahydrofarnesyl acetone), which was not previously detected in Lithophragma. All investigated Lithophragma species produced the (6R, 10R)-enantiomer of this compound. We showed that Greya moths detected not only volatiles of their local Lithophragma plants, but also those from allopatric populations/species that they not encounter in local populations. In conclusion, the generalized detection of volatiles and a lack of co-divergence between volatiles and olfactory detection may be of selective advantage for moths in tracking hosts with rapidly evolving, chemically diverse floral volatiles.</p>},
  author       = {Schiestl, Florian P. and Wallin, Erika A. and Beck, John J. and Friberg, Magne and Thompson, John N.},
  issn         = {1872-8855},
  language     = {eng},
  pages        = {209--221},
  publisher    = {Springer},
  series       = {Arthropod-Plant Interactions},
  title        = {Generalized olfactory detection of floral volatiles in the highly specialized Greya-Lithophragma nursery pollination system},
  url          = {http://dx.doi.org/10.1007/s11829-021-09809-5},
  doi          = {10.1007/s11829-021-09809-5},
  volume       = {15},
  year         = {2021},
}