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Concentrated protein solutions investigated using acoustic levitation and small-Angle X-ray scattering

Sonderby, Pernille ; Soderberg, Christopher LU ; Frankaer, Christian G. ; Peters, Gunther ; Bukrinski, Jens T. ; Labrador, Ana LU orcid ; Plivelic, Tomas S. LU and Harris, Pernille (2020) In Journal of Synchrotron Radiation 27. p.396-404
Abstract

An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml-1. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength... (More)

An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml-1. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength parameter space diagram may be covered easily and the amount of protein sample is significantly reduced (by a factor of 100 in this work). Used in routine measurements, the benefits in terms of protein cost and time spent are very significant.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
concentrated protein solutions, levitated droplet, SAXS, structure factors
in
Journal of Synchrotron Radiation
volume
27
pages
9 pages
publisher
International Union of Crystallography
external identifiers
  • pmid:32153278
  • scopus:85081892819
ISSN
0909-0495
DOI
10.1107/S1600577519016977
language
English
LU publication?
yes
id
074989bd-d253-46e1-bb74-0c2138b00e80
alternative location
https://onlinelibrary.wiley.com/iucr/doi/10.1107/S1600577519016977
date added to LUP
2020-04-17 12:17:37
date last changed
2024-03-04 17:52:49
@article{074989bd-d253-46e1-bb74-0c2138b00e80,
  abstract     = {{<p>An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml<sup>-1</sup>. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength parameter space diagram may be covered easily and the amount of protein sample is significantly reduced (by a factor of 100 in this work). Used in routine measurements, the benefits in terms of protein cost and time spent are very significant.</p>}},
  author       = {{Sonderby, Pernille and Soderberg, Christopher and Frankaer, Christian G. and Peters, Gunther and Bukrinski, Jens T. and Labrador, Ana and Plivelic, Tomas S. and Harris, Pernille}},
  issn         = {{0909-0495}},
  keywords     = {{concentrated protein solutions; levitated droplet; SAXS; structure factors}},
  language     = {{eng}},
  month        = {{03}},
  pages        = {{396--404}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Synchrotron Radiation}},
  title        = {{Concentrated protein solutions investigated using acoustic levitation and small-Angle X-ray scattering}},
  url          = {{http://dx.doi.org/10.1107/S1600577519016977}},
  doi          = {{10.1107/S1600577519016977}},
  volume       = {{27}},
  year         = {{2020}},
}