Concentrated protein solutions investigated using acoustic levitation and small-Angle X-ray scattering
(2020) In Journal of Synchrotron Radiation 27. p.396-404- Abstract
An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml-1. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength... (More)
An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml-1. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength parameter space diagram may be covered easily and the amount of protein sample is significantly reduced (by a factor of 100 in this work). Used in routine measurements, the benefits in terms of protein cost and time spent are very significant.
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- author
- Sonderby, Pernille ; Soderberg, Christopher LU ; Frankaer, Christian G. ; Peters, Gunther ; Bukrinski, Jens T. ; Labrador, Ana LU ; Plivelic, Tomas S. LU and Harris, Pernille
- organization
- publishing date
- 2020-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- concentrated protein solutions, levitated droplet, SAXS, structure factors
- in
- Journal of Synchrotron Radiation
- volume
- 27
- pages
- 9 pages
- publisher
- International Union of Crystallography
- external identifiers
-
- pmid:32153278
- scopus:85081892819
- ISSN
- 0909-0495
- DOI
- 10.1107/S1600577519016977
- language
- English
- LU publication?
- yes
- id
- 074989bd-d253-46e1-bb74-0c2138b00e80
- alternative location
- https://onlinelibrary.wiley.com/iucr/doi/10.1107/S1600577519016977
- date added to LUP
- 2020-04-17 12:17:37
- date last changed
- 2024-03-04 17:52:49
@article{074989bd-d253-46e1-bb74-0c2138b00e80, abstract = {{<p>An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml<sup>-1</sup>. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength parameter space diagram may be covered easily and the amount of protein sample is significantly reduced (by a factor of 100 in this work). Used in routine measurements, the benefits in terms of protein cost and time spent are very significant.</p>}}, author = {{Sonderby, Pernille and Soderberg, Christopher and Frankaer, Christian G. and Peters, Gunther and Bukrinski, Jens T. and Labrador, Ana and Plivelic, Tomas S. and Harris, Pernille}}, issn = {{0909-0495}}, keywords = {{concentrated protein solutions; levitated droplet; SAXS; structure factors}}, language = {{eng}}, month = {{03}}, pages = {{396--404}}, publisher = {{International Union of Crystallography}}, series = {{Journal of Synchrotron Radiation}}, title = {{Concentrated protein solutions investigated using acoustic levitation and small-Angle X-ray scattering}}, url = {{http://dx.doi.org/10.1107/S1600577519016977}}, doi = {{10.1107/S1600577519016977}}, volume = {{27}}, year = {{2020}}, }