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Bacteriophages as biorecognition elements in capacitive biosensors : Phage and host bacteria detection

Ertürk, Gizem LU and Lood, Rolf LU (2018) In Sensors and Actuators, B: Chemical 258. p.535-543
Abstract

Herein, we introduced a molecular imprinting based capacitive biosensor for real-time and highly sensitive bacteriophage detection. The sensing mechanism was based on the binding of target phage into the specific cavities on the electrode surface which resulted in a measurable change in the total capacitance of the system. Phage detection was investigated in the concentration range of 1.0 × 101–1.0 × 105 plaque forming units (pfu)/mL and the limit of detection (LOD) was measured as 10 pfu/mL which shows the high sensitivity of the system compared to results reported for previous studies. The system also allowed the detection of phages in river water samples which is very important for the usability of the system as... (More)

Herein, we introduced a molecular imprinting based capacitive biosensor for real-time and highly sensitive bacteriophage detection. The sensing mechanism was based on the binding of target phage into the specific cavities on the electrode surface which resulted in a measurable change in the total capacitance of the system. Phage detection was investigated in the concentration range of 1.0 × 101–1.0 × 105 plaque forming units (pfu)/mL and the limit of detection (LOD) was measured as 10 pfu/mL which shows the high sensitivity of the system compared to results reported for previous studies. The system also allowed the detection of phages in river water samples which is very important for the usability of the system as in-field analysis for different applications e.g. investigating the contamination of drinking water via wastewater or reservoir water in the future. Recently, due to their high specificity towards their host bacteria, being cost-effective and also stable in harsh environments, bacteriophages have been used as biorecognition elements in many studies. Due to this reason, the applicability of the phage imprinted biosensor was also investigated for host bacteria detection. E. coli detection has been performed in the concentration range of 1.0 × 102–1.0 × 107 colony forming units (cfu)/mL with a LOD value of 100 cfu/mL. This system offers direct, real-time, very sensitive and rapid detection of bacteriophage and its host bacteria.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacteriophage detection, Capacitive biosensor, E. coli detection, In-field analysis, Molecular imprinting
in
Sensors and Actuators, B: Chemical
volume
258
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85037105939
ISSN
0925-4005
DOI
10.1016/j.snb.2017.11.117
language
English
LU publication?
yes
id
075a9573-6c89-437c-924a-cc7ebe6f5dee
date added to LUP
2017-12-20 14:09:43
date last changed
2018-01-25 11:59:56
@article{075a9573-6c89-437c-924a-cc7ebe6f5dee,
  abstract     = {<p>Herein, we introduced a molecular imprinting based capacitive biosensor for real-time and highly sensitive bacteriophage detection. The sensing mechanism was based on the binding of target phage into the specific cavities on the electrode surface which resulted in a measurable change in the total capacitance of the system. Phage detection was investigated in the concentration range of 1.0 × 10<sup>1</sup>–1.0 × 10<sup>5</sup> plaque forming units (pfu)/mL and the limit of detection (LOD) was measured as 10 pfu/mL which shows the high sensitivity of the system compared to results reported for previous studies. The system also allowed the detection of phages in river water samples which is very important for the usability of the system as in-field analysis for different applications e.g. investigating the contamination of drinking water via wastewater or reservoir water in the future. Recently, due to their high specificity towards their host bacteria, being cost-effective and also stable in harsh environments, bacteriophages have been used as biorecognition elements in many studies. Due to this reason, the applicability of the phage imprinted biosensor was also investigated for host bacteria detection. E. coli detection has been performed in the concentration range of 1.0 × 10<sup>2</sup>–1.0 × 10<sup>7</sup> colony forming units (cfu)/mL with a LOD value of 100 cfu/mL. This system offers direct, real-time, very sensitive and rapid detection of bacteriophage and its host bacteria.</p>},
  author       = {Ertürk, Gizem and Lood, Rolf},
  issn         = {0925-4005},
  keyword      = {Bacteriophage detection,Capacitive biosensor,E. coli detection,In-field analysis,Molecular imprinting},
  language     = {eng},
  month        = {04},
  pages        = {535--543},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators, B: Chemical},
  title        = {Bacteriophages as biorecognition elements in capacitive biosensors : Phage and host bacteria detection},
  url          = {http://dx.doi.org/10.1016/j.snb.2017.11.117},
  volume       = {258},
  year         = {2018},
}