Capacitive immunosensor for the detection of host cell proteins.
(2012) In Journal of Biotechnology 157. p.207-213- Abstract
- A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a mode system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby... (More)
- A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a mode system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby reducing negative influence from non-specific binding to the sensor surface. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2220600
- author
- Teeparuksapun, Kosin LU ; Hedström, Martin LU ; Kanatharana, Proespichaya ; Thavarungkul, Panote and Mattiasson, Bo LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biotechnology
- volume
- 157
- pages
- 207 - 213
- publisher
- Elsevier
- external identifiers
-
- wos:000298635200029
- pmid:22100266
- scopus:84855220149
- pmid:22100266
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2011.11.004
- language
- English
- LU publication?
- yes
- id
- 07875e43-18fd-4f98-91dc-7e3bdb7996d9 (old id 2220600)
- date added to LUP
- 2016-04-01 10:22:27
- date last changed
- 2022-04-20 01:32:21
@article{07875e43-18fd-4f98-91dc-7e3bdb7996d9,
abstract = {{A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a mode system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby reducing negative influence from non-specific binding to the sensor surface.}},
author = {{Teeparuksapun, Kosin and Hedström, Martin and Kanatharana, Proespichaya and Thavarungkul, Panote and Mattiasson, Bo}},
issn = {{1873-4863}},
language = {{eng}},
pages = {{207--213}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{Capacitive immunosensor for the detection of host cell proteins.}},
url = {{http://dx.doi.org/10.1016/j.jbiotec.2011.11.004}},
doi = {{10.1016/j.jbiotec.2011.11.004}},
volume = {{157}},
year = {{2012}},
}