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Caffeine consumption attenuates neurochemical modifications in the hippocampus of streptozotocin-induced diabetic rats

Duarte, João M N LU ; Carvalho, Rui A.; Cunha, Rodrigo A and Gruetter, Rolf (2009) In Journal of Neurochemistry 111(2). p.79-368
Abstract

Type 1 diabetes can affect hippocampal function triggering cognitive impairment through unknown mechanisms. Caffeine consumption prevents hippocampal degeneration and memory dysfunction upon different insults and is also known to affect peripheral glucose metabolism. Thus we now characterized glucose transport and the neurochemical profile in the hippocampus of streptozotocin-induced diabetic rats using in vivo(1)H NMR spectroscopy and tested the effect of caffeine consumption thereupon. We found that hippocampal glucose content and transport were unaltered in diabetic rats, irrespective of caffeine consumption. However diabetic rats displayed alterations in their hippocampal neurochemical profile, which were normalized upon restoration... (More)

Type 1 diabetes can affect hippocampal function triggering cognitive impairment through unknown mechanisms. Caffeine consumption prevents hippocampal degeneration and memory dysfunction upon different insults and is also known to affect peripheral glucose metabolism. Thus we now characterized glucose transport and the neurochemical profile in the hippocampus of streptozotocin-induced diabetic rats using in vivo(1)H NMR spectroscopy and tested the effect of caffeine consumption thereupon. We found that hippocampal glucose content and transport were unaltered in diabetic rats, irrespective of caffeine consumption. However diabetic rats displayed alterations in their hippocampal neurochemical profile, which were normalized upon restoration of normoglycaemia, with the exception of myo-inositol that remained increased (36 +/- 5%, p < 0.01 compared to controls) likely reflecting osmolarity deregulation. Compared to controls, caffeine-consuming diabetic rats displayed increased hippocampal levels of myo-inositol (15 +/- 5%, p < 0.05) and taurine (23 +/- 4%, p < 0.01), supporting the ability of caffeine to control osmoregulation. Compared to controls, the hippocampus of diabetic rats displayed a reduced density of synaptic proteins syntaxin, synaptophysin and synaptosome-associated protein of 25 kDa (in average 18 +/- 1%, p < 0.05) as well increased glial fibrillary acidic protein (20 +/- 5%, p < 0.05), suggesting synaptic degeneration and astrogliosis, which were prevented by caffeine consumption. In conclusion, neurochemical alterations in the hippocampus of diabetic rats are not related to defects of glucose transport but likely reflect osmoregulatory adaptations caused by hyperglycemia. Furthermore, caffeine consumption affected this neurochemical adaptation to high glucose levels, which may contribute to its potential neuroprotective effects, namely preventing synaptic degeneration and astrogliosis.

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publishing date
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Contribution to journal
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published
keywords
Animals, Blood Glucose, Caffeine, Central Nervous System Stimulants, Chronic Disease, Diabetes Mellitus, Experimental, Gliosis, Hippocampus, Inositol, Magnetic Resonance Spectroscopy, Male, Models, Biological, Nerve Degeneration, Rats, Rats, Sprague-Dawley, Synapses, Taurine, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Neurochemistry
volume
111
issue
2
pages
12 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:70349338971
ISSN
1471-4159
DOI
10.1111/j.1471-4159.2009.06349.x
language
English
LU publication?
no
id
0794c468-fdc2-4859-8a76-7a02bc950c33
date added to LUP
2017-10-19 15:17:27
date last changed
2017-10-29 05:07:38
@article{0794c468-fdc2-4859-8a76-7a02bc950c33,
  abstract     = {<p>Type 1 diabetes can affect hippocampal function triggering cognitive impairment through unknown mechanisms. Caffeine consumption prevents hippocampal degeneration and memory dysfunction upon different insults and is also known to affect peripheral glucose metabolism. Thus we now characterized glucose transport and the neurochemical profile in the hippocampus of streptozotocin-induced diabetic rats using in vivo(1)H NMR spectroscopy and tested the effect of caffeine consumption thereupon. We found that hippocampal glucose content and transport were unaltered in diabetic rats, irrespective of caffeine consumption. However diabetic rats displayed alterations in their hippocampal neurochemical profile, which were normalized upon restoration of normoglycaemia, with the exception of myo-inositol that remained increased (36 +/- 5%, p &lt; 0.01 compared to controls) likely reflecting osmolarity deregulation. Compared to controls, caffeine-consuming diabetic rats displayed increased hippocampal levels of myo-inositol (15 +/- 5%, p &lt; 0.05) and taurine (23 +/- 4%, p &lt; 0.01), supporting the ability of caffeine to control osmoregulation. Compared to controls, the hippocampus of diabetic rats displayed a reduced density of synaptic proteins syntaxin, synaptophysin and synaptosome-associated protein of 25 kDa (in average 18 +/- 1%, p &lt; 0.05) as well increased glial fibrillary acidic protein (20 +/- 5%, p &lt; 0.05), suggesting synaptic degeneration and astrogliosis, which were prevented by caffeine consumption. In conclusion, neurochemical alterations in the hippocampus of diabetic rats are not related to defects of glucose transport but likely reflect osmoregulatory adaptations caused by hyperglycemia. Furthermore, caffeine consumption affected this neurochemical adaptation to high glucose levels, which may contribute to its potential neuroprotective effects, namely preventing synaptic degeneration and astrogliosis.</p>},
  author       = {Duarte, João M N and Carvalho, Rui A. and Cunha, Rodrigo A and Gruetter, Rolf},
  issn         = {1471-4159},
  keyword      = {Animals,Blood Glucose,Caffeine,Central Nervous System Stimulants,Chronic Disease,Diabetes Mellitus, Experimental,Gliosis,Hippocampus,Inositol,Magnetic Resonance Spectroscopy,Male,Models, Biological,Nerve Degeneration,Rats,Rats, Sprague-Dawley,Synapses,Taurine,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {2},
  pages        = {79--368},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Caffeine consumption attenuates neurochemical modifications in the hippocampus of streptozotocin-induced diabetic rats},
  url          = {http://dx.doi.org/10.1111/j.1471-4159.2009.06349.x},
  volume       = {111},
  year         = {2009},
}