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A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential

Sanzò, Gabriella; Taurino, Irene; Puppo, Francesca; Antiochia, Riccarda; Gorton, Lo LU ; Favero, Gabriele; Mazzei, Franco; Carrara, Sandro and De Micheli, Giovanni (2017) In Methods 129. p.89-95
Abstract

In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with... (More)

In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with nanocoral Au. Moreover, Au-Pt nanostructures were able to lower the potential of detection and a higher performance was obtained at a low applied potential. Then, glucose oxidase was immobilized onto the bimetallic Au-Pt nanostructure using cross-linking with glutaraldehyde. The biosensor was characterized by chronoamperometry at +0.15V vs. Ag pseudo-reference electrode (PRE) and showed good analytical performances with a linear range from 0.01 to 2.00mM and a sensitivity of 33.66μA/mMcm2. The good value of Km app (2.28mM) demonstrates that the hybrid nanostructure is a favorable environment for the enzyme. Moreover, the low working potential can minimize the interference from ascorbic acid and uric acid as well as reducing power consumption to effect sensing. The simple procedure to realize this nanostructure and to immobilize enzymes, as well as the analytical performances of the resulting devices, encourage the use of this technology for the development of biosensors for clinical analysis.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Au nanocoral, Bimetallic hybrid nanostructure, Glucose oxidase, Hydrogen peroxide, Pt nanoflowers, Pt nanospheres
in
Methods
volume
129
pages
89 - 95
publisher
Elsevier
external identifiers
  • scopus:85020382762
ISSN
1046-2023
DOI
10.1016/j.ymeth.2017.06.005
language
English
LU publication?
yes
id
07c60098-a3cc-4091-a1f1-ed89d707f660
date added to LUP
2017-07-03 08:58:20
date last changed
2018-05-20 04:35:20
@article{07c60098-a3cc-4091-a1f1-ed89d707f660,
  abstract     = {<p>In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with nanocoral Au. Moreover, Au-Pt nanostructures were able to lower the potential of detection and a higher performance was obtained at a low applied potential. Then, glucose oxidase was immobilized onto the bimetallic Au-Pt nanostructure using cross-linking with glutaraldehyde. The biosensor was characterized by chronoamperometry at +0.15V vs. Ag pseudo-reference electrode (PRE) and showed good analytical performances with a linear range from 0.01 to 2.00mM and a sensitivity of 33.66μA/mMcm<sup>2</sup>. The good value of K<sub>m</sub> <sup>app</sup> (2.28mM) demonstrates that the hybrid nanostructure is a favorable environment for the enzyme. Moreover, the low working potential can minimize the interference from ascorbic acid and uric acid as well as reducing power consumption to effect sensing. The simple procedure to realize this nanostructure and to immobilize enzymes, as well as the analytical performances of the resulting devices, encourage the use of this technology for the development of biosensors for clinical analysis.</p>},
  author       = {Sanzò, Gabriella and Taurino, Irene and Puppo, Francesca and Antiochia, Riccarda and Gorton, Lo and Favero, Gabriele and Mazzei, Franco and Carrara, Sandro and De Micheli, Giovanni},
  issn         = {1046-2023},
  keyword      = {Au nanocoral,Bimetallic hybrid nanostructure,Glucose oxidase,Hydrogen peroxide,Pt nanoflowers,Pt nanospheres},
  language     = {eng},
  pages        = {89--95},
  publisher    = {Elsevier},
  series       = {Methods},
  title        = {A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H<sub>2</sub>O<sub>2</sub> detection at low potential},
  url          = {http://dx.doi.org/10.1016/j.ymeth.2017.06.005},
  volume       = {129},
  year         = {2017},
}