A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential
(2017) In Methods 129. p.89-95- Abstract
In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with... (More)
In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with nanocoral Au. Moreover, Au-Pt nanostructures were able to lower the potential of detection and a higher performance was obtained at a low applied potential. Then, glucose oxidase was immobilized onto the bimetallic Au-Pt nanostructure using cross-linking with glutaraldehyde. The biosensor was characterized by chronoamperometry at +0.15V vs. Ag pseudo-reference electrode (PRE) and showed good analytical performances with a linear range from 0.01 to 2.00mM and a sensitivity of 33.66μA/mMcm2. The good value of Km app (2.28mM) demonstrates that the hybrid nanostructure is a favorable environment for the enzyme. Moreover, the low working potential can minimize the interference from ascorbic acid and uric acid as well as reducing power consumption to effect sensing. The simple procedure to realize this nanostructure and to immobilize enzymes, as well as the analytical performances of the resulting devices, encourage the use of this technology for the development of biosensors for clinical analysis.
(Less)
- author
- Sanzò, Gabriella ; Taurino, Irene ; Puppo, Francesca ; Antiochia, Riccarda ; Gorton, Lo LU ; Favero, Gabriele ; Mazzei, Franco ; Carrara, Sandro and De Micheli, Giovanni
- organization
- publishing date
- 2017-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Au nanocoral, Bimetallic hybrid nanostructure, Glucose oxidase, Hydrogen peroxide, Pt nanoflowers, Pt nanospheres
- in
- Methods
- volume
- 129
- pages
- 89 - 95
- publisher
- Elsevier
- external identifiers
-
- pmid:28600228
- scopus:85020382762
- ISSN
- 1046-2023
- DOI
- 10.1016/j.ymeth.2017.06.005
- language
- English
- LU publication?
- yes
- id
- 07c60098-a3cc-4091-a1f1-ed89d707f660
- date added to LUP
- 2017-07-03 08:58:20
- date last changed
- 2024-06-09 19:25:15
@article{07c60098-a3cc-4091-a1f1-ed89d707f660,
abstract = {{<p>In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with nanocoral Au. Moreover, Au-Pt nanostructures were able to lower the potential of detection and a higher performance was obtained at a low applied potential. Then, glucose oxidase was immobilized onto the bimetallic Au-Pt nanostructure using cross-linking with glutaraldehyde. The biosensor was characterized by chronoamperometry at +0.15V vs. Ag pseudo-reference electrode (PRE) and showed good analytical performances with a linear range from 0.01 to 2.00mM and a sensitivity of 33.66μA/mMcm<sup>2</sup>. The good value of K<sub>m</sub> <sup>app</sup> (2.28mM) demonstrates that the hybrid nanostructure is a favorable environment for the enzyme. Moreover, the low working potential can minimize the interference from ascorbic acid and uric acid as well as reducing power consumption to effect sensing. The simple procedure to realize this nanostructure and to immobilize enzymes, as well as the analytical performances of the resulting devices, encourage the use of this technology for the development of biosensors for clinical analysis.</p>}},
author = {{Sanzò, Gabriella and Taurino, Irene and Puppo, Francesca and Antiochia, Riccarda and Gorton, Lo and Favero, Gabriele and Mazzei, Franco and Carrara, Sandro and De Micheli, Giovanni}},
issn = {{1046-2023}},
keywords = {{Au nanocoral; Bimetallic hybrid nanostructure; Glucose oxidase; Hydrogen peroxide; Pt nanoflowers; Pt nanospheres}},
language = {{eng}},
pages = {{89--95}},
publisher = {{Elsevier}},
series = {{Methods}},
title = {{A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H<sub>2</sub>O<sub>2</sub> detection at low potential}},
url = {{http://dx.doi.org/10.1016/j.ymeth.2017.06.005}},
doi = {{10.1016/j.ymeth.2017.06.005}},
volume = {{129}},
year = {{2017}},
}