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High Hydrostatic Pressure Induces a Lipid Phase Transition and Molecular Rearrangements in Low-Density Lipoprotein Nanoparticles

Lehofer, Bernhard; Golub, Maksym; Kornmueller, Karin; Kriechbaum, Manfred; Martinez, Nicolas; Nagy, Gergely LU ; Kohlbrecher, Joachim; Amenitsch, Heinz; Peters, Judith and Prassl, Ruth (2018) In Particle and Particle Systems Characterization 35(9).
Abstract

Low-density lipoproteins (LDL) are natural lipid transporter in human plasma whose chemically modified forms contribute to the progression of atherosclerosis and cardiovascular diseases accounting for a vast majority of deaths in westernized civilizations. For the development of new treatment strategies, it is important to have a detailed picture of LDL nanoparticles on a molecular basis. Through the combination of X-ray and neutron small-angle scattering (SAS) techniques with high hydrostatic pressure (HHP) this study describes structural features of normolipidemic, triglyceride-rich and oxidized forms of LDL. Due to the different scattering contrasts for X-rays and neutrons, information on the effects of HHP on the internal structure... (More)

Low-density lipoproteins (LDL) are natural lipid transporter in human plasma whose chemically modified forms contribute to the progression of atherosclerosis and cardiovascular diseases accounting for a vast majority of deaths in westernized civilizations. For the development of new treatment strategies, it is important to have a detailed picture of LDL nanoparticles on a molecular basis. Through the combination of X-ray and neutron small-angle scattering (SAS) techniques with high hydrostatic pressure (HHP) this study describes structural features of normolipidemic, triglyceride-rich and oxidized forms of LDL. Due to the different scattering contrasts for X-rays and neutrons, information on the effects of HHP on the internal structure determined by lipid rearrangements and changes in particle shape becomes accessible. Independent pressure and temperature variations provoke a phase transition in the lipid core domain. With increasing pressure an interrelated anisotropic deformation and flattening of the particle are induced. All LDL nanoparticles maintain their structural integrity even at 3000 bar and show a reversible response toward pressure variations. The present work depicts the complementarity of pressure and temperature as independent thermodynamic parameters and introduces HHP as a tool to study molecular assembling and interaction processes in distinct lipoprotein particles in a nondestructive manner.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
High hydrostatic pressure, Lipid phase transition, Low-density lipoprotein, Nanoparticle structure, Small-angle scattering techniques
in
Particle and Particle Systems Characterization
volume
35
issue
9
publisher
John Wiley & Sons
external identifiers
  • scopus:85050487134
ISSN
0934-0866
DOI
10.1002/ppsc.201800149
language
English
LU publication?
yes
id
07eac7da-63de-4c6d-a6ad-146760eb07d3
date added to LUP
2018-09-11 10:32:27
date last changed
2019-02-20 11:26:16
@article{07eac7da-63de-4c6d-a6ad-146760eb07d3,
  abstract     = {<p>Low-density lipoproteins (LDL) are natural lipid transporter in human plasma whose chemically modified forms contribute to the progression of atherosclerosis and cardiovascular diseases accounting for a vast majority of deaths in westernized civilizations. For the development of new treatment strategies, it is important to have a detailed picture of LDL nanoparticles on a molecular basis. Through the combination of X-ray and neutron small-angle scattering (SAS) techniques with high hydrostatic pressure (HHP) this study describes structural features of normolipidemic, triglyceride-rich and oxidized forms of LDL. Due to the different scattering contrasts for X-rays and neutrons, information on the effects of HHP on the internal structure determined by lipid rearrangements and changes in particle shape becomes accessible. Independent pressure and temperature variations provoke a phase transition in the lipid core domain. With increasing pressure an interrelated anisotropic deformation and flattening of the particle are induced. All LDL nanoparticles maintain their structural integrity even at 3000 bar and show a reversible response toward pressure variations. The present work depicts the complementarity of pressure and temperature as independent thermodynamic parameters and introduces HHP as a tool to study molecular assembling and interaction processes in distinct lipoprotein particles in a nondestructive manner.</p>},
  articleno    = {1800149},
  author       = {Lehofer, Bernhard and Golub, Maksym and Kornmueller, Karin and Kriechbaum, Manfred and Martinez, Nicolas and Nagy, Gergely and Kohlbrecher, Joachim and Amenitsch, Heinz and Peters, Judith and Prassl, Ruth},
  issn         = {0934-0866},
  keyword      = {High hydrostatic pressure,Lipid phase transition,Low-density lipoprotein,Nanoparticle structure,Small-angle scattering techniques},
  language     = {eng},
  month        = {01},
  number       = {9},
  publisher    = {John Wiley & Sons},
  series       = {Particle and Particle Systems Characterization},
  title        = {High Hydrostatic Pressure Induces a Lipid Phase Transition and Molecular Rearrangements in Low-Density Lipoprotein Nanoparticles},
  url          = {http://dx.doi.org/10.1002/ppsc.201800149},
  volume       = {35},
  year         = {2018},
}