Molecular mechanism of cytokinin-activated cell division in Arabidopsis
(2021) In Science 371(6536). p.1350-1355- Abstract
Mitogens trigger cell division in animals. In plants, cytokinins, a group of phytohormones derived from adenine, stimulate cell proliferation. Cytokinin signaling is initiated by membrane-associated histidine kinase receptors and transduced through a phosphorelay system. We show that in the Arabidopsis shoot apical meristem (SAM), cytokinin regulates cell division by promoting nuclear shuttling of Myb-domain protein 3R4 (MYB3R4), a transcription factor that activates mitotic gene expression. Newly synthesized MYB3R4 protein resides predominantly in the cytoplasm. At the G2-to-M transition, rapid nuclear accumulation of MYB3R4-consistent with an associated transient peak in cytokinin concentration-feeds a positive feedback loop involving... (More)
Mitogens trigger cell division in animals. In plants, cytokinins, a group of phytohormones derived from adenine, stimulate cell proliferation. Cytokinin signaling is initiated by membrane-associated histidine kinase receptors and transduced through a phosphorelay system. We show that in the Arabidopsis shoot apical meristem (SAM), cytokinin regulates cell division by promoting nuclear shuttling of Myb-domain protein 3R4 (MYB3R4), a transcription factor that activates mitotic gene expression. Newly synthesized MYB3R4 protein resides predominantly in the cytoplasm. At the G2-to-M transition, rapid nuclear accumulation of MYB3R4-consistent with an associated transient peak in cytokinin concentration-feeds a positive feedback loop involving importins and initiates a transcriptional cascade that drives mitosis and cytokinesis. An engineered nuclear-restricted MYB3R4 mimics the cytokinin effects of enhanced cell proliferation and meristem growth.
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- author
- Yang, Weibing ; Cortijo, Sandra ; Korsbo, Niklas ; Roszak, Pawel ; Schiessl, Katharina ; Gurzadyan, Aram ; Wightman, Raymond ; Jönsson, Henrik LU and Meyerowitz, Elliot
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Science
- volume
- 371
- issue
- 6536
- pages
- 6 pages
- publisher
- American Association for the Advancement of Science (AAAS)
- external identifiers
-
- scopus:85103597987
- pmid:33632892
- ISSN
- 0036-8075
- DOI
- 10.1126/science.abe2305
- language
- English
- LU publication?
- yes
- id
- 08794536-5ff1-4bed-ae99-4764a169fa90
- date added to LUP
- 2021-04-12 13:41:50
- date last changed
- 2024-09-21 18:47:03
@article{08794536-5ff1-4bed-ae99-4764a169fa90, abstract = {{<p>Mitogens trigger cell division in animals. In plants, cytokinins, a group of phytohormones derived from adenine, stimulate cell proliferation. Cytokinin signaling is initiated by membrane-associated histidine kinase receptors and transduced through a phosphorelay system. We show that in the Arabidopsis shoot apical meristem (SAM), cytokinin regulates cell division by promoting nuclear shuttling of Myb-domain protein 3R4 (MYB3R4), a transcription factor that activates mitotic gene expression. Newly synthesized MYB3R4 protein resides predominantly in the cytoplasm. At the G2-to-M transition, rapid nuclear accumulation of MYB3R4-consistent with an associated transient peak in cytokinin concentration-feeds a positive feedback loop involving importins and initiates a transcriptional cascade that drives mitosis and cytokinesis. An engineered nuclear-restricted MYB3R4 mimics the cytokinin effects of enhanced cell proliferation and meristem growth.</p>}}, author = {{Yang, Weibing and Cortijo, Sandra and Korsbo, Niklas and Roszak, Pawel and Schiessl, Katharina and Gurzadyan, Aram and Wightman, Raymond and Jönsson, Henrik and Meyerowitz, Elliot}}, issn = {{0036-8075}}, language = {{eng}}, number = {{6536}}, pages = {{1350--1355}}, publisher = {{American Association for the Advancement of Science (AAAS)}}, series = {{Science}}, title = {{Molecular mechanism of cytokinin-activated cell division in Arabidopsis}}, url = {{http://dx.doi.org/10.1126/science.abe2305}}, doi = {{10.1126/science.abe2305}}, volume = {{371}}, year = {{2021}}, }