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Molecular mechanism of cytokinin-activated cell division in Arabidopsis

Yang, Weibing ; Cortijo, Sandra ; Korsbo, Niklas ; Roszak, Pawel ; Schiessl, Katharina ; Gurzadyan, Aram ; Wightman, Raymond ; Jönsson, Henrik LU and Meyerowitz, Elliot (2021) In Science 371(6536). p.1350-1355
Abstract

Mitogens trigger cell division in animals. In plants, cytokinins, a group of phytohormones derived from adenine, stimulate cell proliferation. Cytokinin signaling is initiated by membrane-associated histidine kinase receptors and transduced through a phosphorelay system. We show that in the Arabidopsis shoot apical meristem (SAM), cytokinin regulates cell division by promoting nuclear shuttling of Myb-domain protein 3R4 (MYB3R4), a transcription factor that activates mitotic gene expression. Newly synthesized MYB3R4 protein resides predominantly in the cytoplasm. At the G2-to-M transition, rapid nuclear accumulation of MYB3R4-consistent with an associated transient peak in cytokinin concentration-feeds a positive feedback loop involving... (More)

Mitogens trigger cell division in animals. In plants, cytokinins, a group of phytohormones derived from adenine, stimulate cell proliferation. Cytokinin signaling is initiated by membrane-associated histidine kinase receptors and transduced through a phosphorelay system. We show that in the Arabidopsis shoot apical meristem (SAM), cytokinin regulates cell division by promoting nuclear shuttling of Myb-domain protein 3R4 (MYB3R4), a transcription factor that activates mitotic gene expression. Newly synthesized MYB3R4 protein resides predominantly in the cytoplasm. At the G2-to-M transition, rapid nuclear accumulation of MYB3R4-consistent with an associated transient peak in cytokinin concentration-feeds a positive feedback loop involving importins and initiates a transcriptional cascade that drives mitosis and cytokinesis. An engineered nuclear-restricted MYB3R4 mimics the cytokinin effects of enhanced cell proliferation and meristem growth.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Science
volume
371
issue
6536
pages
6 pages
publisher
American Association for the Advancement of Science
external identifiers
  • scopus:85103597987
  • pmid:33632892
ISSN
0036-8075
DOI
10.1126/science.abe2305
language
English
LU publication?
yes
id
08794536-5ff1-4bed-ae99-4764a169fa90
date added to LUP
2021-04-12 13:41:50
date last changed
2021-06-08 05:57:07
@article{08794536-5ff1-4bed-ae99-4764a169fa90,
  abstract     = {<p>Mitogens trigger cell division in animals. In plants, cytokinins, a group of phytohormones derived from adenine, stimulate cell proliferation. Cytokinin signaling is initiated by membrane-associated histidine kinase receptors and transduced through a phosphorelay system. We show that in the Arabidopsis shoot apical meristem (SAM), cytokinin regulates cell division by promoting nuclear shuttling of Myb-domain protein 3R4 (MYB3R4), a transcription factor that activates mitotic gene expression. Newly synthesized MYB3R4 protein resides predominantly in the cytoplasm. At the G2-to-M transition, rapid nuclear accumulation of MYB3R4-consistent with an associated transient peak in cytokinin concentration-feeds a positive feedback loop involving importins and initiates a transcriptional cascade that drives mitosis and cytokinesis. An engineered nuclear-restricted MYB3R4 mimics the cytokinin effects of enhanced cell proliferation and meristem growth.</p>},
  author       = {Yang, Weibing and Cortijo, Sandra and Korsbo, Niklas and Roszak, Pawel and Schiessl, Katharina and Gurzadyan, Aram and Wightman, Raymond and Jönsson, Henrik and Meyerowitz, Elliot},
  issn         = {0036-8075},
  language     = {eng},
  number       = {6536},
  pages        = {1350--1355},
  publisher    = {American Association for the Advancement of Science},
  series       = {Science},
  title        = {Molecular mechanism of cytokinin-activated cell division in Arabidopsis},
  url          = {http://dx.doi.org/10.1126/science.abe2305},
  doi          = {10.1126/science.abe2305},
  volume       = {371},
  year         = {2021},
}