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Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Davidsson, Pia; Westman-Brinkmalm, Ann; Nilsson, Carol L LU ; Lindbjer, Maria; Paulson, Linda; Andreasen, Niels; Sjögren, Magnus and Blennow, Kaj LU (2002) In NeuroReport 13(5). p.5-611
Abstract

By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Aged, Aged, 80 and over, Alzheimer Disease, Confidence Intervals, Female, Humans, Longitudinal Studies, Male, Patients, Protein Isoforms, Proteins, Proteome, Statistics, Nonparametric, Comparative Study
in
NeuroReport
volume
13
issue
5
pages
5 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0037117721
ISSN
0959-4965
language
English
LU publication?
no
id
08b8df48-c0b4-4302-a7ac-8cb6e32fe4f9
date added to LUP
2017-05-16 10:41:08
date last changed
2017-10-01 05:34:25
@article{08b8df48-c0b4-4302-a7ac-8cb6e32fe4f9,
  abstract     = {<p>By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.</p>},
  author       = {Davidsson, Pia and Westman-Brinkmalm, Ann and Nilsson, Carol L and Lindbjer, Maria and Paulson, Linda and Andreasen, Niels and Sjögren, Magnus and Blennow, Kaj},
  issn         = {0959-4965},
  keyword      = {Aged,Aged, 80 and over,Alzheimer Disease,Confidence Intervals,Female,Humans,Longitudinal Studies,Male,Patients,Protein Isoforms,Proteins,Proteome,Statistics, Nonparametric,Comparative Study},
  language     = {eng},
  month        = {04},
  number       = {5},
  pages        = {5--611},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients},
  volume       = {13},
  year         = {2002},
}