Possibilities of Using Fetal Hemoglobin as a Platform for Producing Hemoglobin-Based Oxygen Carriers (HBOCs).
(2016) In Advances in Experimental Medicine and Biology 876. p.445-453- Abstract
- The expression levels of fetal hemoglobin (HbF) in bacterial recombinant systems are higher compared with normal adult hemoglobin (HbA). However, heme disorientation in globins are often observed in recombinant production processes, both for HbA and HbF, although the degree of heme oriental disorder is much lower for HbF. In addition, the heme disorientation can be converted to a normal conformation by an oxidation-reduction process. A chromatographic cleaning process involving a strong anion exchanger can be utilized to remove such unstable and nondesirable forms of Hb.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8577081
- author
- Ratanasopa, Khuanpiroon LU ; Cedervall, Tommy LU and Bülow, Leif LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Advances in Experimental Medicine and Biology
- volume
- 876
- pages
- 445 - 453
- publisher
- Springer
- external identifiers
-
- pmid:26782244
- scopus:84958554669
- wos:000373880600057
- pmid:26782244
- ISSN
- 0065-2598
- DOI
- 10.1007/978-1-4939-3023-4_56
- language
- English
- LU publication?
- yes
- id
- 08b9eb1a-51af-400e-ad73-f0b12578d2ff (old id 8577081)
- date added to LUP
- 2016-04-01 13:22:23
- date last changed
- 2022-04-21 21:08:38
@article{08b9eb1a-51af-400e-ad73-f0b12578d2ff, abstract = {{The expression levels of fetal hemoglobin (HbF) in bacterial recombinant systems are higher compared with normal adult hemoglobin (HbA). However, heme disorientation in globins are often observed in recombinant production processes, both for HbA and HbF, although the degree of heme oriental disorder is much lower for HbF. In addition, the heme disorientation can be converted to a normal conformation by an oxidation-reduction process. A chromatographic cleaning process involving a strong anion exchanger can be utilized to remove such unstable and nondesirable forms of Hb.}}, author = {{Ratanasopa, Khuanpiroon and Cedervall, Tommy and Bülow, Leif}}, issn = {{0065-2598}}, language = {{eng}}, pages = {{445--453}}, publisher = {{Springer}}, series = {{Advances in Experimental Medicine and Biology}}, title = {{Possibilities of Using Fetal Hemoglobin as a Platform for Producing Hemoglobin-Based Oxygen Carriers (HBOCs).}}, url = {{http://dx.doi.org/10.1007/978-1-4939-3023-4_56}}, doi = {{10.1007/978-1-4939-3023-4_56}}, volume = {{876}}, year = {{2016}}, }