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Quantitation of bacterial adhesion to polymer surfaces by bioluminescence

Stollenwerk, Maria LU ; Fallgren, Corina LU ; Lundberg, Fredrik LU ; Tegenfeldt, Jonas O. LU ; Montelius, Lars LU and Ljungh, Åsa LU (1998) In Zentralblatt fur Bakteriologie 287(1-2). p.7-18
Abstract

Quantitation of microbes adhering to a surface is commonly used in studies of microbial adhesion to different surfaces. We have quantified different staphylococcal strains adhering to polymer surfaces by measuring bacterial ATP (adenosine triphosphate) by bioluminescence. The method is sensitive, having a detection limit of 104 bacterial cells. Viable counting of bacterial cells may yield falsely low results due to the presence of 'dormant' and adherent bacteria. By using bioluminescence, this can be avoided. Cells of different bacterial species and cells of strains of the same species were shown to differ significantly in their basal ATP content (8.7 x 10-13 - 5.2 x 10-22 MATP). The size of adherent and... (More)

Quantitation of microbes adhering to a surface is commonly used in studies of microbial adhesion to different surfaces. We have quantified different staphylococcal strains adhering to polymer surfaces by measuring bacterial ATP (adenosine triphosphate) by bioluminescence. The method is sensitive, having a detection limit of 104 bacterial cells. Viable counting of bacterial cells may yield falsely low results due to the presence of 'dormant' and adherent bacteria. By using bioluminescence, this can be avoided. Cells of different bacterial species and cells of strains of the same species were shown to differ significantly in their basal ATP content (8.7 x 10-13 - 5.2 x 10-22 MATP). The size of adherent and planktonic bacteria decreased with time (0.7 μm → 0.3 μm, 20 days). During incubation in nutrient-poor buffer ('starvation'), the ATP content of adherent bacteria decreased after 24-96 h whereas that of planktonic bacteria was stable over 20 days. The presence of human serum or plasma did not interfere significantly with the test results. Since the ATP concentration of bacterial strains of different species varies and is also influenced by the growth conditions of bacteria (solid or liquid culture medium), a species-specific standard curve has to be established for bacteria grown under the same culture conditions. We conclude that the method is a sensitive tool to quantify adherent bacteria during experiments lasting for less than 6 h and constitutes a valuable method to be used in conjunction with different microscopical techniques.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Zentralblatt fur Bakteriologie
volume
287
issue
1-2
pages
7 - 18
publisher
Fischer
external identifiers
  • scopus:0031951977
ISSN
0934-8840
DOI
10.1016/S0934-8840(98)80136-9
language
English
LU publication?
yes
id
08c3651b-09c5-4f56-a48b-9852e034eea7
date added to LUP
2018-10-20 10:52:18
date last changed
2019-04-29 14:23:41
@article{08c3651b-09c5-4f56-a48b-9852e034eea7,
  abstract     = {<p>Quantitation of microbes adhering to a surface is commonly used in studies of microbial adhesion to different surfaces. We have quantified different staphylococcal strains adhering to polymer surfaces by measuring bacterial ATP (adenosine triphosphate) by bioluminescence. The method is sensitive, having a detection limit of 10<sup>4</sup> bacterial cells. Viable counting of bacterial cells may yield falsely low results due to the presence of 'dormant' and adherent bacteria. By using bioluminescence, this can be avoided. Cells of different bacterial species and cells of strains of the same species were shown to differ significantly in their basal ATP content (8.7 x 10<sup>-13</sup> - 5.2 x 10<sup>-22</sup> MATP). The size of adherent and planktonic bacteria decreased with time (0.7 μm → 0.3 μm, 20 days). During incubation in nutrient-poor buffer ('starvation'), the ATP content of adherent bacteria decreased after 24-96 h whereas that of planktonic bacteria was stable over 20 days. The presence of human serum or plasma did not interfere significantly with the test results. Since the ATP concentration of bacterial strains of different species varies and is also influenced by the growth conditions of bacteria (solid or liquid culture medium), a species-specific standard curve has to be established for bacteria grown under the same culture conditions. We conclude that the method is a sensitive tool to quantify adherent bacteria during experiments lasting for less than 6 h and constitutes a valuable method to be used in conjunction with different microscopical techniques.</p>},
  author       = {Stollenwerk, Maria and Fallgren, Corina and Lundberg, Fredrik and Tegenfeldt, Jonas O. and Montelius, Lars and Ljungh, Åsa},
  issn         = {0934-8840},
  language     = {eng},
  month        = {01},
  number       = {1-2},
  pages        = {7--18},
  publisher    = {Fischer},
  series       = {Zentralblatt fur Bakteriologie},
  title        = {Quantitation of bacterial adhesion to polymer surfaces by bioluminescence},
  url          = {http://dx.doi.org/10.1016/S0934-8840(98)80136-9},
  volume       = {287},
  year         = {1998},
}