Faster isolation of recombinant factor VIII SQ, with a superporous agarose matrix
(1999) In Journal of chromatography. A 840(1). p.39-50- Abstract
- A superporous agarose matrix was compared with a corresponding homogenous matrix in the isolation of recombinant factor VIII SQ (r-VIII SQ) by immunoaffinity chromatography. As a reference, the commercially available Sepharose FastFlow, used for a similar purification in the industry, was also evaluated. Breakthrough curves are described for flows between 50 and 400 cm/h with pre-purified r-VIII SQ and with cell culture broth. The superporous gel gave the best performance and a 1000-fold purification was obtained in a one-step procedure. The superporous matrix made it possible to increase the throughput about four-fold, presumably due to its better mass transfer properties. The importance of the ligand distribution profile is discussed... (More)
- A superporous agarose matrix was compared with a corresponding homogenous matrix in the isolation of recombinant factor VIII SQ (r-VIII SQ) by immunoaffinity chromatography. As a reference, the commercially available Sepharose FastFlow, used for a similar purification in the industry, was also evaluated. Breakthrough curves are described for flows between 50 and 400 cm/h with pre-purified r-VIII SQ and with cell culture broth. The superporous gel gave the best performance and a 1000-fold purification was obtained in a one-step procedure. The superporous matrix made it possible to increase the throughput about four-fold, presumably due to its better mass transfer properties. The importance of the ligand distribution profile is discussed based upon immunofluorescence microscopy data. (Less)
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https://lup.lub.lu.se/record/09fe7e2f-1943-42b6-b9c3-73df80b2de6d
- author
- Pålsson, Eva ; Smeds, Anna-Lisa ; Peterson, Anna and Larsson, Per-Olof LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Immunoaffinity chromatography, Comparison with commercial adsorbent
- in
- Journal of chromatography. A
- volume
- 840
- issue
- 1
- pages
- 39 - 50
- publisher
- Elsevier
- external identifiers
-
- scopus:0343448992
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(99)00209-5
- language
- English
- LU publication?
- yes
- id
- 09fe7e2f-1943-42b6-b9c3-73df80b2de6d
- date added to LUP
- 2024-05-17 21:52:33
- date last changed
- 2024-05-30 11:32:04
@article{09fe7e2f-1943-42b6-b9c3-73df80b2de6d, abstract = {{A superporous agarose matrix was compared with a corresponding homogenous matrix in the isolation of recombinant factor VIII SQ (r-VIII SQ) by immunoaffinity chromatography. As a reference, the commercially available Sepharose FastFlow, used for a similar purification in the industry, was also evaluated. Breakthrough curves are described for flows between 50 and 400 cm/h with pre-purified r-VIII SQ and with cell culture broth. The superporous gel gave the best performance and a 1000-fold purification was obtained in a one-step procedure. The superporous matrix made it possible to increase the throughput about four-fold, presumably due to its better mass transfer properties. The importance of the ligand distribution profile is discussed based upon immunofluorescence microscopy data.}}, author = {{Pålsson, Eva and Smeds, Anna-Lisa and Peterson, Anna and Larsson, Per-Olof}}, issn = {{0021-9673}}, keywords = {{Immunoaffinity chromatography; Comparison with commercial adsorbent}}, language = {{eng}}, number = {{1}}, pages = {{39--50}}, publisher = {{Elsevier}}, series = {{Journal of chromatography. A}}, title = {{Faster isolation of recombinant factor VIII SQ, with a superporous agarose matrix}}, url = {{http://dx.doi.org/10.1016/S0021-9673(99)00209-5}}, doi = {{10.1016/S0021-9673(99)00209-5}}, volume = {{840}}, year = {{1999}}, }