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Degradation of human secretory immunoglobulin A by Blastocystis

Puthia, Manoj K LU ; Vaithilingam, Aparna; Lu, Jia and Tan, Kevin S W (2005) In Parasitology Research 97(5). p.9-386
Abstract

Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Aspartic Acid Endopeptidases/metabolism, Blastocystis/enzymology, Blastocystis hominis/enzymology, Culture Media, Conditioned/metabolism, Cysteine Endopeptidases/metabolism, Humans, Immunoglobulin A, Secretory/metabolism, Rats
in
Parasitology Research
volume
97
issue
5
pages
4 pages
publisher
Springer
external identifiers
  • scopus:27644585194
ISSN
0932-0113
DOI
10.1007/s00436-005-1461-0
language
English
LU publication?
no
id
0a16dd0c-8380-44cd-abae-f8029b54e18e
date added to LUP
2018-08-27 13:49:37
date last changed
2019-04-10 04:12:15
@article{0a16dd0c-8380-44cd-abae-f8029b54e18e,
  abstract     = {<p>Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.</p>},
  author       = {Puthia, Manoj K and Vaithilingam, Aparna and Lu, Jia and Tan, Kevin S W},
  issn         = {0932-0113},
  keyword      = {Animals,Aspartic Acid Endopeptidases/metabolism,Blastocystis/enzymology,Blastocystis hominis/enzymology,Culture Media, Conditioned/metabolism,Cysteine Endopeptidases/metabolism,Humans,Immunoglobulin A, Secretory/metabolism,Rats},
  language     = {eng},
  number       = {5},
  pages        = {9--386},
  publisher    = {Springer},
  series       = {Parasitology Research},
  title        = {Degradation of human secretory immunoglobulin A by Blastocystis},
  url          = {http://dx.doi.org/10.1007/s00436-005-1461-0},
  volume       = {97},
  year         = {2005},
}