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Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa

Nyström, Max LU ; Westin, Ulla Peterson LU ; Linder, Carina LU and Ohlsson, Kjell (2001) In Mediators of Inflammation 10(5). p.269-272
Abstract

Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique,... (More)

Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Anti-leukoprotease, Human cell culture, Punch biopsy, Trauma
in
Mediators of Inflammation
volume
10
issue
5
pages
269 - 272
publisher
Hindawi Publishing Corporation
external identifiers
  • scopus:0035186271
ISSN
0962-9351
DOI
10.1080/09629350120093740
language
English
LU publication?
yes
id
0a299233-f265-46d1-9214-d4b5fbcda660
date added to LUP
2017-02-13 14:22:04
date last changed
2018-05-29 12:11:09
@article{0a299233-f265-46d1-9214-d4b5fbcda660,
  abstract     = {<p>Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.</p>},
  author       = {Nyström, Max and Westin, Ulla Peterson and Linder, Carina and Ohlsson, Kjell},
  issn         = {0962-9351},
  keyword      = {Anti-leukoprotease,Human cell culture,Punch biopsy,Trauma},
  language     = {eng},
  number       = {5},
  pages        = {269--272},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Mediators of Inflammation},
  title        = {Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa},
  url          = {http://dx.doi.org/10.1080/09629350120093740},
  volume       = {10},
  year         = {2001},
}