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New separation tools for comprehensive studies of protein expression by mass spectrometry

Nilsson, Carol LU and Davidsson, P (2001) In Mass Spectrometry Reviews 19(6). p.7-390
Abstract

Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, peptide mapping, and bioinformatics searches. Using this method, thousands of proteins can be resolved in a gel and their expression quantified. However, certain types of proteins possessing important cellular functions are not easily analyzed using this strategy. These proteins include membrane, low copy number, highly basic, and very large (>... (More)

Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, peptide mapping, and bioinformatics searches. Using this method, thousands of proteins can be resolved in a gel and their expression quantified. However, certain types of proteins possessing important cellular functions are not easily analyzed using this strategy. These proteins include membrane, low copy number, highly basic, and very large (> 150 kDa) and small (< 10 kDa) proteins. To meet the growing need to simultaneously monitor all types of proteins in a biological system, new separation strategies have emerged that are amenable to hyphenation to mass spectrometric techniques. This article will review these new techniques and examine their usefulness in studies of protein expression.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Chromatography, Liquid, Electrophoresis, Humans, Mass Spectrometry, Proteins, Journal Article, Research Support, Non-U.S. Gov't, Review
in
Mass Spectrometry Reviews
volume
19
issue
6
pages
7 - 390
publisher
John Wiley & Sons
external identifiers
  • scopus:0034326966
ISSN
0277-7037
DOI
10.1002/1098-2787(2000)19:6<390::AID-MAS2>3.0.CO;2-1
language
English
LU publication?
no
id
0a3fc3ec-f3b3-4367-9e6a-3d9858644be0
date added to LUP
2017-05-16 10:47:01
date last changed
2018-01-07 12:03:46
@article{0a3fc3ec-f3b3-4367-9e6a-3d9858644be0,
  abstract     = {<p>Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, peptide mapping, and bioinformatics searches. Using this method, thousands of proteins can be resolved in a gel and their expression quantified. However, certain types of proteins possessing important cellular functions are not easily analyzed using this strategy. These proteins include membrane, low copy number, highly basic, and very large (&gt; 150 kDa) and small (&lt; 10 kDa) proteins. To meet the growing need to simultaneously monitor all types of proteins in a biological system, new separation strategies have emerged that are amenable to hyphenation to mass spectrometric techniques. This article will review these new techniques and examine their usefulness in studies of protein expression.</p>},
  author       = {Nilsson, Carol and Davidsson, P},
  issn         = {0277-7037},
  keyword      = {Chromatography, Liquid,Electrophoresis,Humans,Mass Spectrometry,Proteins,Journal Article,Research Support, Non-U.S. Gov't,Review},
  language     = {eng},
  month        = {02},
  number       = {6},
  pages        = {7--390},
  publisher    = {John Wiley & Sons},
  series       = {Mass Spectrometry Reviews},
  title        = {New separation tools for comprehensive studies of protein expression by mass spectrometry},
  url          = {http://dx.doi.org/10.1002/1098-2787(2000)19:6<390::AID-MAS2>3.0.CO;2-1},
  volume       = {19},
  year         = {2001},
}