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Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost

Ravi, Krithika LU ; Garcia Hidalgo, Javier LU ; Gorwa-Grauslund, Marie F LU and Lidén, Gunnar LU (2017) In Applied Microbiology and Biotechnology
Abstract
Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a culture collection. The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h−1) than those on ferulate and vanillate (0.21 and 0.22... (More)
Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a culture collection. The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h−1) than those on ferulate and vanillate (0.21 and 0.22 h−1), as were the uptake rates.There was no direct growth of P. putida KT2440 on vanillin, but instead, vanillin was rapidly converted in to vanillate at a rate of 4.87 mmol (gCDW h)−1 after which the accumulated vanillate was taken up. The growth curve reflected a diauxic growth
when mixtures of the model compounds were used as carbon source. Vanillin, 4-hydroxybenzoate, and benzoate were preferentially consumed first, whereas ferulate was always the last substrate to be taken in. These results contribute to a better understanding of the aromatic metabolism of P. putida in terms of growth and uptake rates, which will be helpful for the utilization of these bacteria as cell factories for upgrading lignin-derived mixtures of aromatic molecules. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Compost, Pseudomonas, Lignin, Aromatic compound conversion, Bacterial metabolism
in
Applied Microbiology and Biotechnology
pages
12 pages
publisher
Springer
external identifiers
  • scopus:85015209095
  • wos:000402712300019
ISSN
1432-0614
DOI
10.1007/s00253-017-8211-y
language
English
LU publication?
yes
id
0a5d9250-aa9b-4a28-af36-45803f1f6bfc
date added to LUP
2017-03-23 08:50:19
date last changed
2018-01-07 11:56:46
@article{0a5d9250-aa9b-4a28-af36-45803f1f6bfc,
  abstract     = {Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with <i>Pseudomonas</i> spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, <i>p</i>-coumarate, benzoate, and ferulate) was carried out with three of the <i>Pseudomonas</i> isolates and in addition with the strain <i>Pseudomonas putida</i> KT2440 from a culture collection. The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h<sup>−1</sup>) than those on ferulate and vanillate (0.21 and 0.22 h<sup>−1</sup>), as were the uptake rates.There was no direct growth of <i>P. putida</i> KT2440 on vanillin, but instead, vanillin was rapidly converted in to vanillate at a rate of 4.87 mmol (gCDW h)<sup>−1</sup> after which the accumulated vanillate was taken up. The growth curve reflected a diauxic growth<br/>when mixtures of the model compounds were used as carbon source. Vanillin, 4-hydroxybenzoate, and benzoate were preferentially consumed first, whereas ferulate was always the last substrate to be taken in. These results contribute to a better understanding of the aromatic metabolism of <i>P. putida</i> in terms of growth and uptake rates, which will be helpful for the utilization of these bacteria as cell factories for upgrading lignin-derived mixtures of aromatic molecules.},
  author       = {Ravi, Krithika and Garcia Hidalgo, Javier and Gorwa-Grauslund, Marie F and Lidén, Gunnar},
  issn         = {1432-0614},
  keyword      = {Compost,Pseudomonas,Lignin,Aromatic compound conversion,Bacterial metabolism},
  language     = {eng},
  month        = {03},
  pages        = {12},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Conversion of lignin model compounds by <i>Pseudomonas putida</i> KT2440 and isolates from compost},
  url          = {http://dx.doi.org/10.1007/s00253-017-8211-y},
  year         = {2017},
}