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Uropathogenic Escherichia coli strain CFT073 disrupts NLRP3 inflammasome activation

Waldhuber, Anna; Puthia, Manoj LU ; Wieser, Andreas; Cirl, Christine; Dürr, Susanne; Neumann-Pfeifer, Silke; Albrecht, Simone; Römmler, Franziska; Müller, Tina and Zheng, Yunji, et al. (2016) In Journal of Clinical Investigation 126(7). p.36-2425
Abstract

Successful bacterial pathogens produce an array of virulence factors that allow subversion of the immune system and persistence within the host. For example, uropathogenic Escherichia coli strains, such as CFT073, express Toll/IL-1 receptor-containing (TIR-containing) protein C (TcpC), which impairs TLR signaling, thereby suppressing innate immunity in the urinary tract and enhancing persistence in the kidneys. Here, we have reported that TcpC also reduces secretion of IL-1β by directly interacting with the NACHT leucin-rich repeat PYD protein 3 (NLRP3) inflammasome, which is crucial for recognition of pathogens within the cytosol. At a low MOI, IL-1β secretion was minimal in CFT073-infected macrophages; however, IL-1β release was... (More)

Successful bacterial pathogens produce an array of virulence factors that allow subversion of the immune system and persistence within the host. For example, uropathogenic Escherichia coli strains, such as CFT073, express Toll/IL-1 receptor-containing (TIR-containing) protein C (TcpC), which impairs TLR signaling, thereby suppressing innate immunity in the urinary tract and enhancing persistence in the kidneys. Here, we have reported that TcpC also reduces secretion of IL-1β by directly interacting with the NACHT leucin-rich repeat PYD protein 3 (NLRP3) inflammasome, which is crucial for recognition of pathogens within the cytosol. At a low MOI, IL-1β secretion was minimal in CFT073-infected macrophages; however, IL-1β release was markedly increased in macrophages infected with CFT073 lacking tcpC. Induction of IL-1β secretion by CFT073 and tcpC-deficient CFT073 required the NLRP3 inflammasome. TcpC attenuated activation of the NLRP3 inflammasome by binding both NLRP3 and caspase-1 and thereby preventing processing and activation of caspase-1. Moreover, in a murine urinary tract infection model, CFT073 infection rapidly induced expression of the NLRP3 inflammasome in the bladder mucosa; however, the presence of TcpC in WT CFT073 reduced IL-1β levels in the urine of infected mice. Together, these findings illustrate how uropathogenic E. coli use the multifunctional virulence factor TcpC to attenuate innate immune responses in the urinary tract.

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Journal of Clinical Investigation
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126
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7
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12 pages
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The Journal of Clinical Investigation
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  • scopus:84978437406
ISSN
0021-9738
DOI
10.1172/JCI81916
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English
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0aa73efa-1821-4eef-adfa-a4dcf3d6c471
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4922710/
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2017-09-14 10:10:47
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@article{0aa73efa-1821-4eef-adfa-a4dcf3d6c471,
  abstract     = {<p>Successful bacterial pathogens produce an array of virulence factors that allow subversion of the immune system and persistence within the host. For example, uropathogenic Escherichia coli strains, such as CFT073, express Toll/IL-1 receptor-containing (TIR-containing) protein C (TcpC), which impairs TLR signaling, thereby suppressing innate immunity in the urinary tract and enhancing persistence in the kidneys. Here, we have reported that TcpC also reduces secretion of IL-1β by directly interacting with the NACHT leucin-rich repeat PYD protein 3 (NLRP3) inflammasome, which is crucial for recognition of pathogens within the cytosol. At a low MOI, IL-1β secretion was minimal in CFT073-infected macrophages; however, IL-1β release was markedly increased in macrophages infected with CFT073 lacking tcpC. Induction of IL-1β secretion by CFT073 and tcpC-deficient CFT073 required the NLRP3 inflammasome. TcpC attenuated activation of the NLRP3 inflammasome by binding both NLRP3 and caspase-1 and thereby preventing processing and activation of caspase-1. Moreover, in a murine urinary tract infection model, CFT073 infection rapidly induced expression of the NLRP3 inflammasome in the bladder mucosa; however, the presence of TcpC in WT CFT073 reduced IL-1β levels in the urine of infected mice. Together, these findings illustrate how uropathogenic E. coli use the multifunctional virulence factor TcpC to attenuate innate immune responses in the urinary tract.</p>},
  author       = {Waldhuber, Anna and Puthia, Manoj and Wieser, Andreas and Cirl, Christine and Dürr, Susanne and Neumann-Pfeifer, Silke and Albrecht, Simone and Römmler, Franziska and Müller, Tina and Zheng, Yunji and Schubert, Sören and Groß, Olaf and Svanborg, Catharina and Miethke, Thomas},
  issn         = {0021-9738},
  keyword      = {Journal Article},
  language     = {eng},
  month        = {07},
  number       = {7},
  pages        = {36--2425},
  publisher    = {The Journal of Clinical Investigation},
  series       = {Journal of Clinical Investigation},
  title        = {Uropathogenic Escherichia coli strain CFT073 disrupts NLRP3 inflammasome activation},
  url          = {http://dx.doi.org/10.1172/JCI81916},
  volume       = {126},
  year         = {2016},
}