A heterotrinuclear bioinspired coordination complex capable of binding to DNA and emulation of nuclease activity
(2022) In Journal of Inorganic Biochemistry 226.- Abstract
The investigation of compounds capable of strongly and selectively interacting with DNA comprises a field of research in constant development. In this work, we demonstrate that a trinuclear coordination complex based on a dinuclear Fe(III)Zn(II) core designed for biomimicry of the hydrolytic enzyme kidney bean purple acid phosphatase, containing an additional pendant arm coordinating a Pd(II) ion, has the ability to interact with DNA and to promote its hydrolytic cleavage. These results were found through analysis of plasmid DNA interaction and cleavage by the trinuclear complex 1 and its derivatives 2 and 3, in addition to the analysis of alteration in the DNA structure in the presence of the complexes through circular dichroism and... (More)
The investigation of compounds capable of strongly and selectively interacting with DNA comprises a field of research in constant development. In this work, we demonstrate that a trinuclear coordination complex based on a dinuclear Fe(III)Zn(II) core designed for biomimicry of the hydrolytic enzyme kidney bean purple acid phosphatase, containing an additional pendant arm coordinating a Pd(II) ion, has the ability to interact with DNA and to promote its hydrolytic cleavage. These results were found through analysis of plasmid DNA interaction and cleavage by the trinuclear complex 1 and its derivatives 2 and 3, in addition to the analysis of alteration in the DNA structure in the presence of the complexes through circular dichroism and DNA footprinting techniques. The suggested covalent interaction of the palladium-containing complex with DNA was analysed using an electrophoretic mobility assay, circular dichroism, high resolution gel separation techniques and kinetic analysis. This is a new and promising metal complex targeted to nucleic acids and acting in two separate ways: strong DNA interaction and hydrolytic cleavage.
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- author
- Gabriel, Philipe ; Maranha, Filipy Gobbo ; Nordlander, Ebbe LU ; Neves, Ademir and Terenzi, Hernán
- organization
- publishing date
- 2022-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Artificial nuclease, Biomimetic, Covalent interaction, DNA cleavage, DNA interaction, Metallonuclease
- in
- Journal of Inorganic Biochemistry
- volume
- 226
- article number
- 111631
- publisher
- Elsevier
- external identifiers
-
- scopus:85117914595
- pmid:34717251
- ISSN
- 0162-0134
- DOI
- 10.1016/j.jinorgbio.2021.111631
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: The authors thank the CAPES / STINT Joint Brazilian-Swedish Collaboration program for a grant ( BR 2013-5218 ). We also thanks CNPq , FINEP and CAPES for financial support. Publisher Copyright: © 2021 Elsevier Inc.
- id
- 0b18b862-b893-4d01-83bc-6cf112f94817
- date added to LUP
- 2021-11-12 12:52:21
- date last changed
- 2024-04-06 12:59:07
@article{0b18b862-b893-4d01-83bc-6cf112f94817,
abstract = {{<p>The investigation of compounds capable of strongly and selectively interacting with DNA comprises a field of research in constant development. In this work, we demonstrate that a trinuclear coordination complex based on a dinuclear Fe(III)Zn(II) core designed for biomimicry of the hydrolytic enzyme kidney bean purple acid phosphatase, containing an additional pendant arm coordinating a Pd(II) ion, has the ability to interact with DNA and to promote its hydrolytic cleavage. These results were found through analysis of plasmid DNA interaction and cleavage by the trinuclear complex 1 and its derivatives 2 and 3, in addition to the analysis of alteration in the DNA structure in the presence of the complexes through circular dichroism and DNA footprinting techniques. The suggested covalent interaction of the palladium-containing complex with DNA was analysed using an electrophoretic mobility assay, circular dichroism, high resolution gel separation techniques and kinetic analysis. This is a new and promising metal complex targeted to nucleic acids and acting in two separate ways: strong DNA interaction and hydrolytic cleavage.</p>}},
author = {{Gabriel, Philipe and Maranha, Filipy Gobbo and Nordlander, Ebbe and Neves, Ademir and Terenzi, Hernán}},
issn = {{0162-0134}},
keywords = {{Artificial nuclease; Biomimetic; Covalent interaction; DNA cleavage; DNA interaction; Metallonuclease}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Journal of Inorganic Biochemistry}},
title = {{A heterotrinuclear bioinspired coordination complex capable of binding to DNA and emulation of nuclease activity}},
url = {{http://dx.doi.org/10.1016/j.jinorgbio.2021.111631}},
doi = {{10.1016/j.jinorgbio.2021.111631}},
volume = {{226}},
year = {{2022}},
}