Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake
(2010) In Genes & Development 24(14). p.5-1491- Abstract
Inactivation of the yeast nonsense-mediated mRNA decay (NMD) pathway stabilizes nonsense mRNAs and promotes readthrough of premature translation termination codons. Although the latter phenotype is thought to reflect a direct role of NMD factors in translation termination, its mechanism is unknown. Here we show that the reduced termination efficiency of NMD-deficient cells is attributable to increased expression of the magnesium transporter Alr1p and the resulting effects of elevated Mg(2+) levels on termination fidelity. Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg(2+) homeostasis and, consequently, translational fidelity.
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https://lup.lub.lu.se/record/0b2b8b79-1f8f-40f6-885f-70b2233a8c4f
- author
- Johansson, Marcus J O LU and Jacobson, Allan
- publishing date
- 2010-07-15
- type
- Contribution to journal
- publication status
- published
- keywords
- 5' Untranslated Regions, Cation Transport Proteins/genetics, Codon, Nonsense, Magnesium/metabolism, Open Reading Frames, Protein Biosynthesis, RNA Stability, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics
- in
- Genes & Development
- volume
- 24
- issue
- 14
- pages
- 5 - 1491
- publisher
- Cold Spring Harbor Laboratory Press (CSHL)
- external identifiers
-
- pmid:20634315
- scopus:77954856723
- ISSN
- 1549-5477
- DOI
- 10.1101/gad.1930710
- language
- English
- LU publication?
- no
- id
- 0b2b8b79-1f8f-40f6-885f-70b2233a8c4f
- date added to LUP
- 2024-03-05 16:33:03
- date last changed
- 2024-06-13 12:37:02
@article{0b2b8b79-1f8f-40f6-885f-70b2233a8c4f, abstract = {{<p>Inactivation of the yeast nonsense-mediated mRNA decay (NMD) pathway stabilizes nonsense mRNAs and promotes readthrough of premature translation termination codons. Although the latter phenotype is thought to reflect a direct role of NMD factors in translation termination, its mechanism is unknown. Here we show that the reduced termination efficiency of NMD-deficient cells is attributable to increased expression of the magnesium transporter Alr1p and the resulting effects of elevated Mg(2+) levels on termination fidelity. Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg(2+) homeostasis and, consequently, translational fidelity.</p>}}, author = {{Johansson, Marcus J O and Jacobson, Allan}}, issn = {{1549-5477}}, keywords = {{5' Untranslated Regions; Cation Transport Proteins/genetics; Codon, Nonsense; Magnesium/metabolism; Open Reading Frames; Protein Biosynthesis; RNA Stability; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics}}, language = {{eng}}, month = {{07}}, number = {{14}}, pages = {{5--1491}}, publisher = {{Cold Spring Harbor Laboratory Press (CSHL)}}, series = {{Genes & Development}}, title = {{Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake}}, url = {{http://dx.doi.org/10.1101/gad.1930710}}, doi = {{10.1101/gad.1930710}}, volume = {{24}}, year = {{2010}}, }