Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake

Johansson, Marcus J O LU and Jacobson, Allan (2010) In Genes & Development 24(14). p.5-1491
Abstract

Inactivation of the yeast nonsense-mediated mRNA decay (NMD) pathway stabilizes nonsense mRNAs and promotes readthrough of premature translation termination codons. Although the latter phenotype is thought to reflect a direct role of NMD factors in translation termination, its mechanism is unknown. Here we show that the reduced termination efficiency of NMD-deficient cells is attributable to increased expression of the magnesium transporter Alr1p and the resulting effects of elevated Mg(2+) levels on termination fidelity. Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg(2+) homeostasis and, consequently, translational fidelity.

Please use this url to cite or link to this publication:
author
and
publishing date
type
Contribution to journal
publication status
published
keywords
5' Untranslated Regions, Cation Transport Proteins/genetics, Codon, Nonsense, Magnesium/metabolism, Open Reading Frames, Protein Biosynthesis, RNA Stability, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics
in
Genes & Development
volume
24
issue
14
pages
5 - 1491
publisher
Cold Spring Harbor Laboratory Press (CSHL)
external identifiers
  • pmid:20634315
  • scopus:77954856723
ISSN
1549-5477
DOI
10.1101/gad.1930710
language
English
LU publication?
no
id
0b2b8b79-1f8f-40f6-885f-70b2233a8c4f
date added to LUP
2024-03-05 16:33:03
date last changed
2024-06-13 12:37:02
@article{0b2b8b79-1f8f-40f6-885f-70b2233a8c4f,
  abstract     = {{<p>Inactivation of the yeast nonsense-mediated mRNA decay (NMD) pathway stabilizes nonsense mRNAs and promotes readthrough of premature translation termination codons. Although the latter phenotype is thought to reflect a direct role of NMD factors in translation termination, its mechanism is unknown. Here we show that the reduced termination efficiency of NMD-deficient cells is attributable to increased expression of the magnesium transporter Alr1p and the resulting effects of elevated Mg(2+) levels on termination fidelity. Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg(2+) homeostasis and, consequently, translational fidelity.</p>}},
  author       = {{Johansson, Marcus J O and Jacobson, Allan}},
  issn         = {{1549-5477}},
  keywords     = {{5' Untranslated Regions; Cation Transport Proteins/genetics; Codon, Nonsense; Magnesium/metabolism; Open Reading Frames; Protein Biosynthesis; RNA Stability; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{14}},
  pages        = {{5--1491}},
  publisher    = {{Cold Spring Harbor Laboratory Press (CSHL)}},
  series       = {{Genes & Development}},
  title        = {{Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake}},
  url          = {{http://dx.doi.org/10.1101/gad.1930710}},
  doi          = {{10.1101/gad.1930710}},
  volume       = {{24}},
  year         = {{2010}},
}