UnCovid : A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection
(2021) In STAR Protocols 2(4).- Abstract
Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0bd99f2b-24f8-4bc2-b00f-bc408b4f01eb
- author
- Alcántara, Roberto ; Peñaranda, Katherin ; Mendoza-Rojas, Gabriel ; Nakamoto, Jose A LU ; Dueñas, Eva ; Alvarez, Daniela ; Adaui, Vanessa and Milón, Pohl
- publishing date
- 2021-12-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- COVID-19/diagnosis, CRISPR-Cas Systems, Humans, Nucleic Acid Amplification Techniques/methods, RNA, Viral/analysis, SARS-CoV-2/genetics
- in
- STAR Protocols
- volume
- 2
- issue
- 4
- article number
- 100878
- publisher
- Cell Press
- external identifiers
-
- scopus:85118485880
- pmid:34604812
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2021.100878
- language
- English
- LU publication?
- no
- additional info
- © 2021 The Author(s).
- id
- 0bd99f2b-24f8-4bc2-b00f-bc408b4f01eb
- date added to LUP
- 2022-10-13 10:29:26
- date last changed
- 2024-04-19 19:53:34
@article{0bd99f2b-24f8-4bc2-b00f-bc408b4f01eb, abstract = {{<p>Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.</p>}}, author = {{Alcántara, Roberto and Peñaranda, Katherin and Mendoza-Rojas, Gabriel and Nakamoto, Jose A and Dueñas, Eva and Alvarez, Daniela and Adaui, Vanessa and Milón, Pohl}}, issn = {{2666-1667}}, keywords = {{COVID-19/diagnosis; CRISPR-Cas Systems; Humans; Nucleic Acid Amplification Techniques/methods; RNA, Viral/analysis; SARS-CoV-2/genetics}}, language = {{eng}}, month = {{12}}, number = {{4}}, publisher = {{Cell Press}}, series = {{STAR Protocols}}, title = {{UnCovid : A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection}}, url = {{http://dx.doi.org/10.1016/j.xpro.2021.100878}}, doi = {{10.1016/j.xpro.2021.100878}}, volume = {{2}}, year = {{2021}}, }