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UnCovid : A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection

Alcántara, Roberto ; Peñaranda, Katherin ; Mendoza-Rojas, Gabriel ; Nakamoto, Jose A LU orcid ; Dueñas, Eva ; Alvarez, Daniela ; Adaui, Vanessa and Milón, Pohl (2021) In STAR Protocols 2(4).
Abstract

Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.

Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
COVID-19/diagnosis, CRISPR-Cas Systems, Humans, Nucleic Acid Amplification Techniques/methods, RNA, Viral/analysis, SARS-CoV-2/genetics
in
STAR Protocols
volume
2
issue
4
article number
100878
publisher
Cell Press
external identifiers
  • scopus:85118485880
  • pmid:34604812
ISSN
2666-1667
DOI
10.1016/j.xpro.2021.100878
language
English
LU publication?
no
additional info
© 2021 The Author(s).
id
0bd99f2b-24f8-4bc2-b00f-bc408b4f01eb
date added to LUP
2022-10-13 10:29:26
date last changed
2024-04-19 19:53:34
@article{0bd99f2b-24f8-4bc2-b00f-bc408b4f01eb,
  abstract     = {{<p>Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach remote regions, this work was developed to use the portable molecular workstation from BentoLab. For complete details on the use and execution of this protocol, please refer to Alcántara et al., 2021.</p>}},
  author       = {{Alcántara, Roberto and Peñaranda, Katherin and Mendoza-Rojas, Gabriel and Nakamoto, Jose A and Dueñas, Eva and Alvarez, Daniela and Adaui, Vanessa and Milón, Pohl}},
  issn         = {{2666-1667}},
  keywords     = {{COVID-19/diagnosis; CRISPR-Cas Systems; Humans; Nucleic Acid Amplification Techniques/methods; RNA, Viral/analysis; SARS-CoV-2/genetics}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{4}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{UnCovid : A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2021.100878}},
  doi          = {{10.1016/j.xpro.2021.100878}},
  volume       = {{2}},
  year         = {{2021}},
}