Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter
(2009) In Biochemistry 48(21). p.505-4497- Abstract
In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions. Phosphate addition causes Pho84-mediated activation of the protein kinase A (PKA) pathway as well as rapid internalization and vacuolar breakdown of Pho84. We show that Pho84 undergoes phosphate-induced phosphorylation and subsequent ubiquitination on amino acids located in the large middle intracellular loop prior to endocytosis. The attachment of ubiquitin is dependent on the ubiquitin conjugating enzymes Ubc2 and Ubc4. In addition, we show that the Pho84 endocytotic process is delayed in strains with reduced PKA activity. Our results suggest that Pho84-mediated activation of the PKA pathway... (More)
In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions. Phosphate addition causes Pho84-mediated activation of the protein kinase A (PKA) pathway as well as rapid internalization and vacuolar breakdown of Pho84. We show that Pho84 undergoes phosphate-induced phosphorylation and subsequent ubiquitination on amino acids located in the large middle intracellular loop prior to endocytosis. The attachment of ubiquitin is dependent on the ubiquitin conjugating enzymes Ubc2 and Ubc4. In addition, we show that the Pho84 endocytotic process is delayed in strains with reduced PKA activity. Our results suggest that Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown.
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- author
- Lundh, Fredrik ; Mouillon, Jean-Marie ; Samyn, Dieter ; Stadler, Kent ; Popova, Yulia ; Lagerstedt, Jens O LU ; Thevelein, Johan M and Persson, Bengt L
- publishing date
- 2009-06-02
- type
- Contribution to journal
- publication status
- published
- keywords
- Cyclic AMP-Dependent Protein Kinases, Down-Regulation, Feedback, Physiological, Intracellular Space, Phosphates, Phosphorylation, Protein Transport, Proton-Phosphate Symporters, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Ubiquitin, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't
- in
- Biochemistry
- volume
- 48
- issue
- 21
- pages
- 9 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:19348508
- scopus:66349086108
- ISSN
- 0006-2960
- DOI
- 10.1021/bi9001198
- language
- English
- LU publication?
- no
- id
- 0c5dd5c8-53a2-4bc3-a6f1-b1b4de3264a3
- date added to LUP
- 2017-10-19 20:09:42
- date last changed
- 2024-06-10 01:59:55
@article{0c5dd5c8-53a2-4bc3-a6f1-b1b4de3264a3,
abstract = {{<p>In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions. Phosphate addition causes Pho84-mediated activation of the protein kinase A (PKA) pathway as well as rapid internalization and vacuolar breakdown of Pho84. We show that Pho84 undergoes phosphate-induced phosphorylation and subsequent ubiquitination on amino acids located in the large middle intracellular loop prior to endocytosis. The attachment of ubiquitin is dependent on the ubiquitin conjugating enzymes Ubc2 and Ubc4. In addition, we show that the Pho84 endocytotic process is delayed in strains with reduced PKA activity. Our results suggest that Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown.</p>}},
author = {{Lundh, Fredrik and Mouillon, Jean-Marie and Samyn, Dieter and Stadler, Kent and Popova, Yulia and Lagerstedt, Jens O and Thevelein, Johan M and Persson, Bengt L}},
issn = {{0006-2960}},
keywords = {{Cyclic AMP-Dependent Protein Kinases; Down-Regulation; Feedback, Physiological; Intracellular Space; Phosphates; Phosphorylation; Protein Transport; Proton-Phosphate Symporters; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Signal Transduction; Ubiquitin; Up-Regulation; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{06}},
number = {{21}},
pages = {{505--4497}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Biochemistry}},
title = {{Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter}},
url = {{http://dx.doi.org/10.1021/bi9001198}},
doi = {{10.1021/bi9001198}},
volume = {{48}},
year = {{2009}},
}