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Gas chromatographic analysis of cellular fatty acids in anaerobic curved bacteria isolated from the vagina

Skarin, A LU ; Larsson, L LU ; Holst, E LU and Mårdh, P A LU (1984) In Scandinavian Journal of Urology 86(supplement). p.4-71
Abstract

The fatty acid compositions of forty-five strains of anaerobic curved rods isolated from vaginal content were analysed by gas chromatography. Twenty-six of the strains were of a short Gram-variable type and 19 of a long Gram-negative type. The bacteria were subjected to alkaline saponification, after which the liberated fatty acids were methylated using boron trichloride-methanol. The samples were introduced onto a cross-linked (SE-54) fused silica capillary column. The major peaks in the chromatograms from all strains corresponded to C14:0, C16:0, C18:2, C18:1 and C18:0 fatty acids. Five additional components were seen, three of them identified by mass spectrometry as fatty aldehydes (saturated and unsaturated) of 16 to 18 carbon... (More)

The fatty acid compositions of forty-five strains of anaerobic curved rods isolated from vaginal content were analysed by gas chromatography. Twenty-six of the strains were of a short Gram-variable type and 19 of a long Gram-negative type. The bacteria were subjected to alkaline saponification, after which the liberated fatty acids were methylated using boron trichloride-methanol. The samples were introduced onto a cross-linked (SE-54) fused silica capillary column. The major peaks in the chromatograms from all strains corresponded to C14:0, C16:0, C18:2, C18:1 and C18:0 fatty acids. Five additional components were seen, three of them identified by mass spectrometry as fatty aldehydes (saturated and unsaturated) of 16 to 18 carbon atoms, whereas the remaining two were not identified. The fatty aldehydes indicated the presence of plasmalogens in the bacterial strains studied. With two exceptions, all of the short rods were distinguishable from the long ones by the presence of one aldehyde and two unidentified compounds, all three eluting between C16:0 and C18:2.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacteria, Anaerobic/analysis, Chromatography, Gas, Fatty Acids/analysis, Female, Humans, Vagina/microbiology
in
Scandinavian Journal of Urology
volume
86
issue
supplement
pages
4 - 71
publisher
Taylor & Francis
external identifiers
  • scopus:0021550934
  • pmid:6598925
ISSN
0300-8886
language
English
LU publication?
yes
id
0d16f7f0-765d-45e4-9fd1-8e22d9a6e018
date added to LUP
2022-07-12 09:31:20
date last changed
2026-08-24 16:18:23
@article{0d16f7f0-765d-45e4-9fd1-8e22d9a6e018,
  abstract     = {{<p>The fatty acid compositions of forty-five strains of anaerobic curved rods isolated from vaginal content were analysed by gas chromatography. Twenty-six of the strains were of a short Gram-variable type and 19 of a long Gram-negative type. The bacteria were subjected to alkaline saponification, after which the liberated fatty acids were methylated using boron trichloride-methanol. The samples were introduced onto a cross-linked (SE-54) fused silica capillary column. The major peaks in the chromatograms from all strains corresponded to C14:0, C16:0, C18:2, C18:1 and C18:0 fatty acids. Five additional components were seen, three of them identified by mass spectrometry as fatty aldehydes (saturated and unsaturated) of 16 to 18 carbon atoms, whereas the remaining two were not identified. The fatty aldehydes indicated the presence of plasmalogens in the bacterial strains studied. With two exceptions, all of the short rods were distinguishable from the long ones by the presence of one aldehyde and two unidentified compounds, all three eluting between C16:0 and C18:2.</p>}},
  author       = {{Skarin, A and Larsson, L and Holst, E and Mårdh, P A}},
  issn         = {{0300-8886}},
  keywords     = {{Bacteria, Anaerobic/analysis; Chromatography, Gas; Fatty Acids/analysis; Female; Humans; Vagina/microbiology}},
  language     = {{eng}},
  number       = {{supplement}},
  pages        = {{4--71}},
  publisher    = {{Taylor & Francis}},
  series       = {{Scandinavian Journal of Urology}},
  title        = {{Gas chromatographic analysis of cellular fatty acids in anaerobic curved bacteria isolated from the vagina}},
  volume       = {{86}},
  year         = {{1984}},
}