From (p)ppGpp to (pp)pGpp : Characterization of regulatory effects of pGpp synthesized by the small alarmone synthetase of Enterococcus faecalis
(2015) In Journal of Bacteriology 197(18). p.2908-2919- Abstract
The bacterial stringent response (SR) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. This response is mediated by the intracellular accumulation of the alarmones pppGpp and ppGpp, collectively called (p)ppGpp. In Enterococcus faecalis, (p)ppGpp metabolism is carried out by the bifunctional synthetase/hydrolase E. faecalis Rel (RelEf) and the small alarmone synthetase (SAS) RelQEf. Although Rel is the main enzyme responsible for SR activation in Firmicutes, there is emerging evidence that SASs can make important contributions to bacterial homeostasis. Here, we showed that RelQEf synthesizes ppGpp more efficiently than pppGpp without the need... (More)
The bacterial stringent response (SR) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. This response is mediated by the intracellular accumulation of the alarmones pppGpp and ppGpp, collectively called (p)ppGpp. In Enterococcus faecalis, (p)ppGpp metabolism is carried out by the bifunctional synthetase/hydrolase E. faecalis Rel (RelEf) and the small alarmone synthetase (SAS) RelQEf. Although Rel is the main enzyme responsible for SR activation in Firmicutes, there is emerging evidence that SASs can make important contributions to bacterial homeostasis. Here, we showed that RelQEf synthesizes ppGpp more efficiently than pppGpp without the need for ribosomes, tRNA, or mRNA. In addition to (p)ppGpp synthesis from GDP and GTP, RelQEf also efficiently utilized GMP to form GMP 3'-diphosphate (pGpp). Based on this observation, we sought to determine if pGpp exerts regulatory effects on cellular processes affected by (p)ppGpp. We found that pGpp, like (p)ppGpp, strongly inhibits the activity of E. faecalis enzymes involved in GTP biosynthesis and, to a lesser extent, transcription of rrnB by Escherichia coli RNA polymerase. Activation of E. coli RelA synthetase activity was observed in the presence of both pGpp and ppGpp, while RelQEf was activated only by ppGpp. Furthermore, enzymatic activity of RelQEf is insensitive to relacin, a (p)ppGpp analog developed as an inhibitor of "long" RelA/SpoT homolog (RSH) enzymes. We conclude that pGpp can likely function as a bacterial alarmone with target-specific regulatory effects that are similar to what has been observed for (p)ppGpp.
(Less)
- author
- Gaca, Anthony O.
; Kudrin, Pavel
; Colomer-Winter, Cristina
; Beljantseva, Jelena
; Liu, Kuanqing
; Anderson, Brent
; Wang, Jue D.
; Rejman, Dominik
; Potrykus, Katarzyna
; Cashel, Michael
; Hauryliuk, Vasili
LU
and Lemos, José A.
(Less) - publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bacteriology
- volume
- 197
- issue
- 18
- pages
- 12 pages
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:26124242
- scopus:84940416485
- ISSN
- 0021-9193
- DOI
- 10.1128/JB.00324-15
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2015, American Society for Microbiology. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
- id
- 0d2d520f-bf23-4233-9541-b1c0737a7eb4
- date added to LUP
- 2021-09-24 20:45:21
- date last changed
- 2024-06-15 17:19:04
@article{0d2d520f-bf23-4233-9541-b1c0737a7eb4,
abstract = {{<p>The bacterial stringent response (SR) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. This response is mediated by the intracellular accumulation of the alarmones pppGpp and ppGpp, collectively called (p)ppGpp. In Enterococcus faecalis, (p)ppGpp metabolism is carried out by the bifunctional synthetase/hydrolase E. faecalis Rel (Rel<sub>Ef</sub>) and the small alarmone synthetase (SAS) RelQ<sub>Ef</sub>. Although Rel is the main enzyme responsible for SR activation in Firmicutes, there is emerging evidence that SASs can make important contributions to bacterial homeostasis. Here, we showed that RelQ<sub>Ef</sub> synthesizes ppGpp more efficiently than pppGpp without the need for ribosomes, tRNA, or mRNA. In addition to (p)ppGpp synthesis from GDP and GTP, RelQ<sub>Ef</sub> also efficiently utilized GMP to form GMP 3'-diphosphate (pGpp). Based on this observation, we sought to determine if pGpp exerts regulatory effects on cellular processes affected by (p)ppGpp. We found that pGpp, like (p)ppGpp, strongly inhibits the activity of E. faecalis enzymes involved in GTP biosynthesis and, to a lesser extent, transcription of rrnB by Escherichia coli RNA polymerase. Activation of E. coli RelA synthetase activity was observed in the presence of both pGpp and ppGpp, while RelQ<sub>Ef</sub> was activated only by ppGpp. Furthermore, enzymatic activity of RelQ<sub>Ef</sub> is insensitive to relacin, a (p)ppGpp analog developed as an inhibitor of "long" RelA/SpoT homolog (RSH) enzymes. We conclude that pGpp can likely function as a bacterial alarmone with target-specific regulatory effects that are similar to what has been observed for (p)ppGpp.</p>}},
author = {{Gaca, Anthony O. and Kudrin, Pavel and Colomer-Winter, Cristina and Beljantseva, Jelena and Liu, Kuanqing and Anderson, Brent and Wang, Jue D. and Rejman, Dominik and Potrykus, Katarzyna and Cashel, Michael and Hauryliuk, Vasili and Lemos, José A.}},
issn = {{0021-9193}},
language = {{eng}},
number = {{18}},
pages = {{2908--2919}},
publisher = {{American Society for Microbiology}},
series = {{Journal of Bacteriology}},
title = {{From (p)ppGpp to (pp)pGpp : Characterization of regulatory effects of pGpp synthesized by the small alarmone synthetase of Enterococcus faecalis}},
url = {{http://dx.doi.org/10.1128/JB.00324-15}},
doi = {{10.1128/JB.00324-15}},
volume = {{197}},
year = {{2015}},
}