Rifabutin accumulates in the lens and reduces retinal function in the rabbit eye
(2009) In Retina 29(1). p.106-111- Abstract
PURPOSE: To study the toxicology of rifabutin in the rabbit eye with emphasis on retinal function and histopathology.
METHODS: Seven rabbits received a daily dose of rifabutin during 15 months. Six rabbits receiving only the vehicle were used as controls. Repeated standardized full-field electroretinograms (ERG) were assessed. After discontinuing treatment, the rabbits were killed and the cornea, the lens, and the sectioned retina was studied. Immunhistochemistry directed against vimentin, glial fibrillaryacidic protein (GFAP), protein kinase C (PKC), and peanut agglutinin (PNA) was performed.
RESULTS: Rifabutin was detected in serum of the treated rabbits. During treatment, the full-field ERG demonstrated significantly... (More)
PURPOSE: To study the toxicology of rifabutin in the rabbit eye with emphasis on retinal function and histopathology.
METHODS: Seven rabbits received a daily dose of rifabutin during 15 months. Six rabbits receiving only the vehicle were used as controls. Repeated standardized full-field electroretinograms (ERG) were assessed. After discontinuing treatment, the rabbits were killed and the cornea, the lens, and the sectioned retina was studied. Immunhistochemistry directed against vimentin, glial fibrillaryacidic protein (GFAP), protein kinase C (PKC), and peanut agglutinin (PNA) was performed.
RESULTS: Rifabutin was detected in serum of the treated rabbits. During treatment, the full-field ERG demonstrated significantly reduced b-wave amplitudes in the total rod-cone response as well as in the isolated rod and cone response compared with the recordings before treatment. The control rabbits did not demonstrate a reduction of the ERG amplitudes. The treated rabbits developed a discoloration of the lens, not seen in the control group. No retinal pathology was demonstrated using immunohistochemical methods.
CONCLUSION: Rifabutin causes a discoloration of the lens and reduces both rod and cone function in rabbits, but does not alter retinal morphology. Previous reports on ocular side effects caused by rifabutin are supported by the results of this study.
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- author
- Cardiakidis Myers, Anna LU ; Kjellström, Sten LU ; Bruun, Anitha LU ; Isaksson, Björn LU ; Ghosh, Fredrik LU ; Andréasson, Sten LU and Ponjavic, Vesna LU
- organization
- publishing date
- 2009-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Anti-Bacterial Agents, Electroretinography, Female, Fluorescent Antibody Technique, Indirect, Glial Fibrillary Acidic Protein, Lens, Crystalline, Male, Protein Kinase C, Rabbits, Retina, Retinal Diseases, Rifabutin, Vimentin, Journal Article, Research Support, Non-U.S. Gov't
- in
- Retina
- volume
- 29
- issue
- 1
- pages
- 6 pages
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000262413300017
- pmid:18685541
- scopus:61549105919
- pmid:18685541
- ISSN
- 0275-004X
- DOI
- 10.1097/IAE.0b013e318180ab3c
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200), Ophthalmology (Lund) (013043000)
- id
- 0d5b4156-1509-4bf7-8d8d-b53de0ff3c1e (old id 1223423)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18685541?dopt=Abstract
- date added to LUP
- 2016-04-04 09:43:20
- date last changed
- 2022-04-23 22:00:26
@article{0d5b4156-1509-4bf7-8d8d-b53de0ff3c1e,
abstract = {{<p>PURPOSE: To study the toxicology of rifabutin in the rabbit eye with emphasis on retinal function and histopathology.</p><p>METHODS: Seven rabbits received a daily dose of rifabutin during 15 months. Six rabbits receiving only the vehicle were used as controls. Repeated standardized full-field electroretinograms (ERG) were assessed. After discontinuing treatment, the rabbits were killed and the cornea, the lens, and the sectioned retina was studied. Immunhistochemistry directed against vimentin, glial fibrillaryacidic protein (GFAP), protein kinase C (PKC), and peanut agglutinin (PNA) was performed.</p><p>RESULTS: Rifabutin was detected in serum of the treated rabbits. During treatment, the full-field ERG demonstrated significantly reduced b-wave amplitudes in the total rod-cone response as well as in the isolated rod and cone response compared with the recordings before treatment. The control rabbits did not demonstrate a reduction of the ERG amplitudes. The treated rabbits developed a discoloration of the lens, not seen in the control group. No retinal pathology was demonstrated using immunohistochemical methods.</p><p>CONCLUSION: Rifabutin causes a discoloration of the lens and reduces both rod and cone function in rabbits, but does not alter retinal morphology. Previous reports on ocular side effects caused by rifabutin are supported by the results of this study.</p>}},
author = {{Cardiakidis Myers, Anna and Kjellström, Sten and Bruun, Anitha and Isaksson, Björn and Ghosh, Fredrik and Andréasson, Sten and Ponjavic, Vesna}},
issn = {{0275-004X}},
keywords = {{Animals; Anti-Bacterial Agents; Electroretinography; Female; Fluorescent Antibody Technique, Indirect; Glial Fibrillary Acidic Protein; Lens, Crystalline; Male; Protein Kinase C; Rabbits; Retina; Retinal Diseases; Rifabutin; Vimentin; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{1}},
pages = {{106--111}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{Retina}},
title = {{Rifabutin accumulates in the lens and reduces retinal function in the rabbit eye}},
url = {{http://dx.doi.org/10.1097/IAE.0b013e318180ab3c}},
doi = {{10.1097/IAE.0b013e318180ab3c}},
volume = {{29}},
year = {{2009}},
}