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High-throughput magnetic resonance imaging in murine colonic inflammation

Melgar, Silvia; Gillberg, Per-Göran; Hockings, Paul D and Olsson, Lars E LU (2007) In Biochemical and Biophysical Research Communications 355(4). p.7-1102
Abstract

The aim of this study was to investigate if a rapid magnetic resonance imaging (MRI) screening protocol (<5min/mouse) could characterize colonic inflammation in a chronic experimental colitis model. No respiratory triggering or spasmolytic agent was used during MRI-acquisition. Biomarkers assessed in vivo were colon wall thickness and T2w signal intensity (reflecting oedema) and ex vivo inflammatory score, colon weight, and plasma haptoglobin. The inflammation was characterised by significantly higher local and systemic inflammatory markers in the colitic mice compared to healthy mice. MRI-colon wall thickness and T2w signal intensity correlated well with inflammatory score (r=0.95 and 0.94), colon weight (r=0.92 and 0.93) and plasma... (More)

The aim of this study was to investigate if a rapid magnetic resonance imaging (MRI) screening protocol (<5min/mouse) could characterize colonic inflammation in a chronic experimental colitis model. No respiratory triggering or spasmolytic agent was used during MRI-acquisition. Biomarkers assessed in vivo were colon wall thickness and T2w signal intensity (reflecting oedema) and ex vivo inflammatory score, colon weight, and plasma haptoglobin. The inflammation was characterised by significantly higher local and systemic inflammatory markers in the colitic mice compared to healthy mice. MRI-colon wall thickness and T2w signal intensity correlated well with inflammatory score (r=0.95 and 0.94), colon weight (r=0.92 and 0.93) and plasma haptoglobin (r=0.89 and 0.95). Thus, the data showed that in vivo MRI screening could be used to assess colon wall inflammation, suggesting that high-throughput MRI can be used to follow the potential efficacy of new IBD therapies in individual animal in longitudinal studies.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Body Weight, Colitis, Disease Models, Animal, Female, Magnetic Resonance Imaging, Mice, Mice, Inbred C57BL
in
Biochemical and Biophysical Research Communications
volume
355
issue
4
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:33847657465
ISSN
0006-291X
DOI
10.1016/j.bbrc.2007.02.090
language
English
LU publication?
no
id
0d807300-bc9b-4a24-a12a-ee124985a9b3
date added to LUP
2016-08-16 12:11:30
date last changed
2017-01-01 08:31:44
@article{0d807300-bc9b-4a24-a12a-ee124985a9b3,
  abstract     = {<p>The aim of this study was to investigate if a rapid magnetic resonance imaging (MRI) screening protocol (&lt;5min/mouse) could characterize colonic inflammation in a chronic experimental colitis model. No respiratory triggering or spasmolytic agent was used during MRI-acquisition. Biomarkers assessed in vivo were colon wall thickness and T2w signal intensity (reflecting oedema) and ex vivo inflammatory score, colon weight, and plasma haptoglobin. The inflammation was characterised by significantly higher local and systemic inflammatory markers in the colitic mice compared to healthy mice. MRI-colon wall thickness and T2w signal intensity correlated well with inflammatory score (r=0.95 and 0.94), colon weight (r=0.92 and 0.93) and plasma haptoglobin (r=0.89 and 0.95). Thus, the data showed that in vivo MRI screening could be used to assess colon wall inflammation, suggesting that high-throughput MRI can be used to follow the potential efficacy of new IBD therapies in individual animal in longitudinal studies.</p>},
  author       = {Melgar, Silvia and Gillberg, Per-Göran and Hockings, Paul D and Olsson, Lars E},
  issn         = {0006-291X},
  keyword      = {Animals,Body Weight,Colitis,Disease Models, Animal,Female,Magnetic Resonance Imaging,Mice,Mice, Inbred C57BL},
  language     = {eng},
  month        = {04},
  number       = {4},
  pages        = {7--1102},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {High-throughput magnetic resonance imaging in murine colonic inflammation},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2007.02.090},
  volume       = {355},
  year         = {2007},
}