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Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene

Marote, Ana LU ; Pomeshchik, Yuriy LU orcid ; Collin, Anna LU ; Goldwurm, Stefano ; Lamas, Nuno J. LU ; Pinto, Luísa ; Salgado, António J. and Roybon, Laurent LU (2018) In Stem Cell Research 28. p.44-47
Abstract

The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cell Research
volume
28
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85041562765
  • pmid:29414418
ISSN
1873-5061
DOI
10.1016/j.scr.2018.01.022
language
English
LU publication?
yes
id
0d8837db-8d04-4609-8faa-a7e22a98562c
date added to LUP
2018-02-20 10:21:10
date last changed
2024-03-18 05:15:12
@article{0d8837db-8d04-4609-8faa-a7e22a98562c,
  abstract     = {{<p>The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.</p>}},
  author       = {{Marote, Ana and Pomeshchik, Yuriy and Collin, Anna and Goldwurm, Stefano and Lamas, Nuno J. and Pinto, Luísa and Salgado, António J. and Roybon, Laurent}},
  issn         = {{1873-5061}},
  language     = {{eng}},
  month        = {{04}},
  pages        = {{44--47}},
  publisher    = {{Elsevier}},
  series       = {{Stem Cell Research}},
  title        = {{Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene}},
  url          = {{http://dx.doi.org/10.1016/j.scr.2018.01.022}},
  doi          = {{10.1016/j.scr.2018.01.022}},
  volume       = {{28}},
  year         = {{2018}},
}