Advanced

Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene

Marote, Ana LU ; Pomeshchik, Yuriy LU ; Collin, Anna LU ; Goldwurm, Stefano; Lamas, Nuno J. LU ; Pinto, Luísa; Salgado, António J. and Roybon, Laurent LU (2018) In Stem Cell Research 28. p.44-47
Abstract

The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cell Research
volume
28
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85041562765
ISSN
1873-5061
DOI
10.1016/j.scr.2018.01.022
language
English
LU publication?
yes
id
0d8837db-8d04-4609-8faa-a7e22a98562c
date added to LUP
2018-02-20 10:21:10
date last changed
2018-05-29 09:51:25
@article{0d8837db-8d04-4609-8faa-a7e22a98562c,
  abstract     = {<p>The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.</p>},
  author       = {Marote, Ana and Pomeshchik, Yuriy and Collin, Anna and Goldwurm, Stefano and Lamas, Nuno J. and Pinto, Luísa and Salgado, António J. and Roybon, Laurent},
  issn         = {1873-5061},
  language     = {eng},
  month        = {04},
  pages        = {44--47},
  publisher    = {Elsevier},
  series       = {Stem Cell Research},
  title        = {Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene},
  url          = {http://dx.doi.org/10.1016/j.scr.2018.01.022},
  volume       = {28},
  year         = {2018},
}