Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene
(2018) In Stem Cell Research 28. p.44-47- Abstract
The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0d8837db-8d04-4609-8faa-a7e22a98562c
- author
- Marote, Ana LU ; Pomeshchik, Yuriy LU ; Collin, Anna LU ; Goldwurm, Stefano ; Lamas, Nuno J. LU ; Pinto, Luísa ; Salgado, António J. and Roybon, Laurent LU
- organization
- publishing date
- 2018-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Stem Cell Research
- volume
- 28
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:29414418
- scopus:85041562765
- ISSN
- 1873-5061
- DOI
- 10.1016/j.scr.2018.01.022
- language
- English
- LU publication?
- yes
- id
- 0d8837db-8d04-4609-8faa-a7e22a98562c
- date added to LUP
- 2018-02-20 10:21:10
- date last changed
- 2024-07-08 09:30:25
@article{0d8837db-8d04-4609-8faa-a7e22a98562c, abstract = {{<p>The leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription factors. The generated iPSC line exhibits expression of common pluripotency markers, differentiates into the three germ layers and has a normal karyotype. The iPSC line can be used to explore the association between LRRK2 mutation and PD.</p>}}, author = {{Marote, Ana and Pomeshchik, Yuriy and Collin, Anna and Goldwurm, Stefano and Lamas, Nuno J. and Pinto, Luísa and Salgado, António J. and Roybon, Laurent}}, issn = {{1873-5061}}, language = {{eng}}, month = {{04}}, pages = {{44--47}}, publisher = {{Elsevier}}, series = {{Stem Cell Research}}, title = {{Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's disease patient carrying a p.G2019S mutation in the LRRK2 gene}}, url = {{http://dx.doi.org/10.1016/j.scr.2018.01.022}}, doi = {{10.1016/j.scr.2018.01.022}}, volume = {{28}}, year = {{2018}}, }