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Effects of phosphorus in growth media on biomineralization and cell surface properties of marine cyanobacteria synechococcus

Paulo, Carlos; Kenney, Janice P.L.; Persson, Per LU and Dittrich, Maria (2018) In Geosciences (Switzerland) 8(12).
Abstract


Through geological time, cyanobacterial picoplankton have impacted the global carbon cycle by sequestrating CO
2
and forming authigenic carbonate minerals. Various studies have emphasized the cyanobacterial cell envelopes as nucleation sites for calcium carbonate formation. Little is known, however, about how environmental conditions (e.g., nutrient content) trigger a cell surface and its properties and, consequently, influence biomineralization. Our study aims to understand how phosphorus (P) concentration impacts the properties of cell... (More)


Through geological time, cyanobacterial picoplankton have impacted the global carbon cycle by sequestrating CO
2
and forming authigenic carbonate minerals. Various studies have emphasized the cyanobacterial cell envelopes as nucleation sites for calcium carbonate formation. Little is known, however, about how environmental conditions (e.g., nutrient content) trigger a cell surface and its properties and, consequently, influence biomineralization. Our study aims to understand how phosphorus (P) concentration impacts the properties of cell surfaces and cell–mineral interactions. Changes to the surface properties of marine Synechococcus strains grown under various P conditions were characterized by potentiometric titrations, X-ray photoelectron spectroscopy (XPS), and tip-enhanced Raman spectroscopy (TERS). Biomineralization experiments were performed using cyanobacterial cells, which were grown under different P concentrations and exposed to solutions slightly oversaturated with respect to calcium carbonate. We observed the changes induced by different P conditions in the macromolecular composition of the cyanobacteria cell envelope and its consequences for biomineralization. The modified properties of cell surfaces were linked to carbonate precipitation rates and mineral morphology from biomineralization experiments. Our analysis shows that the increase of phosphoryl groups and surface charge, as well as the relative proportion of polysaccharides and proteins, can impact carbonate precipitation by picocyanobacteria.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Calcium carbonate biomineralization, Carbonate, Cyanobacteria, Infrared spectroscopy, Phosphorus, Synechococcus cells, Tip-enhanced Raman spectroscopy, X-ray photoelectron spectroscopy
in
Geosciences (Switzerland)
volume
8
issue
12
publisher
MDPI AG
external identifiers
  • scopus:85062385928
DOI
10.3390/geosciences8120471
language
English
LU publication?
yes
id
0d975d7f-87b6-4bdb-af4a-4180da161ce3
date added to LUP
2019-03-15 08:02:37
date last changed
2019-04-10 04:21:06
@article{0d975d7f-87b6-4bdb-af4a-4180da161ce3,
  abstract     = {<p><br>
                                                         Through geological time, cyanobacterial picoplankton have impacted the global carbon cycle by sequestrating CO                             <br>
                            <sub>2</sub><br>
                                                          and forming authigenic carbonate minerals. Various studies have emphasized the cyanobacterial cell envelopes as nucleation sites for calcium carbonate formation. Little is known, however, about how environmental conditions (e.g., nutrient content) trigger a cell surface and its properties and, consequently, influence biomineralization. Our study aims to understand how phosphorus (P) concentration impacts the properties of cell surfaces and cell–mineral interactions. Changes to the surface properties of marine Synechococcus strains grown under various P conditions were characterized by potentiometric titrations, X-ray photoelectron spectroscopy (XPS), and tip-enhanced Raman spectroscopy (TERS). Biomineralization experiments were performed using cyanobacterial cells, which were grown under different P concentrations and exposed to solutions slightly oversaturated with respect to calcium carbonate. We observed the changes induced by different P conditions in the macromolecular composition of the cyanobacteria cell envelope and its consequences for biomineralization. The modified properties of cell surfaces were linked to carbonate precipitation rates and mineral morphology from biomineralization experiments. Our analysis shows that the increase of phosphoryl groups and surface charge, as well as the relative proportion of polysaccharides and proteins, can impact carbonate precipitation by picocyanobacteria.                         <br>
                        </p>},
  articleno    = {471},
  author       = {Paulo, Carlos and Kenney, Janice P.L. and Persson, Per and Dittrich, Maria},
  keyword      = {Calcium carbonate biomineralization,Carbonate,Cyanobacteria,Infrared spectroscopy,Phosphorus,Synechococcus cells,Tip-enhanced Raman spectroscopy,X-ray photoelectron spectroscopy},
  language     = {eng},
  month        = {12},
  number       = {12},
  publisher    = {MDPI AG},
  series       = {Geosciences (Switzerland)},
  title        = {Effects of phosphorus in growth media on biomineralization and cell surface properties of marine cyanobacteria synechococcus},
  url          = {http://dx.doi.org/10.3390/geosciences8120471},
  volume       = {8},
  year         = {2018},
}