Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Immunoglobulin from insulin-dependent diabetic children inhibits glucose-induced insulin release

Kanatsuna, T. ; Baekkeskov, S. ; Lernmark, A. LU orcid and Ludvigsson, J. (1983) In Diabetes 32(6 I). p.520-524
Abstract

Immunoglobulin was separated from islet cel antibody positive plasma of six children with newly diagnosed insulin-dependent (type I) diabetes mellitus. The dynamics of insulin release in response to glucose and partially purified antibodies were determined in dispersed rat islet cells perifused on small columns of Biogel P-2 beads. After perifusion at 5.5 mmol/L D-glucose in the presence of healthy control immunoglobulin, the rate of insulin release increased in a biphasic manner after stimulation with 30 mmol/L D-glucose. In cells exposed to diabetic immunoglobulin, 30 mmol/L D-glucose had little, if any, effect on insulin release. These results suggest that islet cell antibodies in insulin-dependent diabetes may interfere with the... (More)

Immunoglobulin was separated from islet cel antibody positive plasma of six children with newly diagnosed insulin-dependent (type I) diabetes mellitus. The dynamics of insulin release in response to glucose and partially purified antibodies were determined in dispersed rat islet cells perifused on small columns of Biogel P-2 beads. After perifusion at 5.5 mmol/L D-glucose in the presence of healthy control immunoglobulin, the rate of insulin release increased in a biphasic manner after stimulation with 30 mmol/L D-glucose. In cells exposed to diabetic immunoglobulin, 30 mmol/L D-glucose had little, if any, effect on insulin release. These results suggest that islet cell antibodies in insulin-dependent diabetes may interfere with the insulin release mechanisms in the pancreatic B-cells.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
publishing date
type
Contribution to journal
publication status
published
in
Diabetes
volume
32
issue
6 I
pages
5 pages
publisher
American Diabetes Association Inc.
external identifiers
  • scopus:0020577258
  • pmid:6354781
ISSN
0012-1797
language
English
LU publication?
no
id
0dcefcb0-52d7-4bd2-8852-9531a63ce81c
date added to LUP
2019-09-16 13:24:45
date last changed
2024-03-13 08:24:40
@article{0dcefcb0-52d7-4bd2-8852-9531a63ce81c,
  abstract     = {{<p>Immunoglobulin was separated from islet cel antibody positive plasma of six children with newly diagnosed insulin-dependent (type I) diabetes mellitus. The dynamics of insulin release in response to glucose and partially purified antibodies were determined in dispersed rat islet cells perifused on small columns of Biogel P-2 beads. After perifusion at 5.5 mmol/L D-glucose in the presence of healthy control immunoglobulin, the rate of insulin release increased in a biphasic manner after stimulation with 30 mmol/L D-glucose. In cells exposed to diabetic immunoglobulin, 30 mmol/L D-glucose had little, if any, effect on insulin release. These results suggest that islet cell antibodies in insulin-dependent diabetes may interfere with the insulin release mechanisms in the pancreatic B-cells.</p>}},
  author       = {{Kanatsuna, T. and Baekkeskov, S. and Lernmark, A. and Ludvigsson, J.}},
  issn         = {{0012-1797}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{6 I}},
  pages        = {{520--524}},
  publisher    = {{American Diabetes Association Inc.}},
  series       = {{Diabetes}},
  title        = {{Immunoglobulin from insulin-dependent diabetic children inhibits glucose-induced insulin release}},
  volume       = {{32}},
  year         = {{1983}},
}