Nanoscale Structural Characterization of Individual Viral Particles Using Atomic Force Microscope Infrared (AFM-IR) and Tip-Enhanced Raman Spectroscopy (TERS)
Dou, Tianyi ; Li, Zhandong ; Zhang, Junjie ; Evilevitch, Alex LU
and Kurouski, Dmitry
(2020)
In Analytical Chemistry
92(16).
p.11297-11304
- Abstract
Viruses are infections species that infect a large spectrum of living systems. Although displaying a wide variety of shapes and sizes, they are all composed of nucleic acid encapsulated into a protein capsid. After virions enter the host cell, they replicate to produce multiple copies of themselves. They then lyse the host, releasing virions to infect new cells. High proliferation rate of viruses is the underlying cause of their fast transmission among living species. Although many viruses are harmless, some of them are responsible for severe diseases such as AIDS, viral hepatitis and flu. Traditionally, electron microscopy is used to identify and characterize viruses. This approach is time and labor consuming, which is problematic upon... (More)
Viruses are infections species that infect a large spectrum of living systems. Although displaying a wide variety of shapes and sizes, they are all composed of nucleic acid encapsulated into a protein capsid. After virions enter the host cell, they replicate to produce multiple copies of themselves. They then lyse the host, releasing virions to infect new cells. High proliferation rate of viruses is the underlying cause of their fast transmission among living species. Although many viruses are harmless, some of them are responsible for severe diseases such as AIDS, viral hepatitis and flu. Traditionally, electron microscopy is used to identify and characterize viruses. This approach is time and labor consuming, which is problematic upon pandemic proliferation of previously unknown viruses. Herein, we demonstrate a novel diagnosis approach for label-free identification and structural characterization of individual viruses that is based on a combination of nanoscale Raman and Infrared spectroscopy. Using atomic force microscopy infrared spectroscopy (AFM-IR), we were able to probe structural organization of the virions of herpes simplex type 1 viruses and bacteriophage MS2. We also showed that tip enhanced Raman spectroscopy could be used to reveal protein secondary structure and amino acid composition of the virus surface. Our results show that AFM-IR and TERS provide different but complimentary information about the structure of complex biological specimens. This structural information can be used for fast and reliable identification of viruses. This nanoscale bimodal imaging approach can be also used to investigate the origin of viral polymorphism and study mechanisms of virion assembly.
(Less)
- author
- Dou, Tianyi
; Li, Zhandong
; Zhang, Junjie
; Evilevitch, Alex
LU
and Kurouski, Dmitry
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 92
- issue
- 16
- pages
- 11297 - 11304
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85091911802
- pmid:32683857
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.0c01971
- language
- English
- LU publication?
- yes
- id
- 0dde2256-80c7-4424-bc00-aa8e0b6067da
- date added to LUP
- 2020-07-28 18:33:50
- date last changed
- 2024-04-03 10:45:50
@article{0dde2256-80c7-4424-bc00-aa8e0b6067da,
abstract = {{<p>Viruses are infections species that infect a large spectrum of living systems. Although displaying a wide variety of shapes and sizes, they are all composed of nucleic acid encapsulated into a protein capsid. After virions enter the host cell, they replicate to produce multiple copies of themselves. They then lyse the host, releasing virions to infect new cells. High proliferation rate of viruses is the underlying cause of their fast transmission among living species. Although many viruses are harmless, some of them are responsible for severe diseases such as AIDS, viral hepatitis and flu. Traditionally, electron microscopy is used to identify and characterize viruses. This approach is time and labor consuming, which is problematic upon pandemic proliferation of previously unknown viruses. Herein, we demonstrate a novel diagnosis approach for label-free identification and structural characterization of individual viruses that is based on a combination of nanoscale Raman and Infrared spectroscopy. Using atomic force microscopy infrared spectroscopy (AFM-IR), we were able to probe structural organization of the virions of herpes simplex type 1 viruses and bacteriophage MS2. We also showed that tip enhanced Raman spectroscopy could be used to reveal protein secondary structure and amino acid composition of the virus surface. Our results show that AFM-IR and TERS provide different but complimentary information about the structure of complex biological specimens. This structural information can be used for fast and reliable identification of viruses. This nanoscale bimodal imaging approach can be also used to investigate the origin of viral polymorphism and study mechanisms of virion assembly.</p>}},
author = {{Dou, Tianyi and Li, Zhandong and Zhang, Junjie and Evilevitch, Alex and Kurouski, Dmitry}},
issn = {{1520-6882}},
language = {{eng}},
number = {{16}},
pages = {{11297--11304}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Nanoscale Structural Characterization of Individual Viral Particles Using Atomic Force Microscope Infrared (AFM-IR) and Tip-Enhanced Raman Spectroscopy (TERS)}},
url = {{http://dx.doi.org/10.1021/acs.analchem.0c01971}},
doi = {{10.1021/acs.analchem.0c01971}},
volume = {{92}},
year = {{2020}},
}