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APETALA2 control of barley internode elongation

Patil, Vrushali ; McDermott, Hannah I. ; McAllister, Trisha ; Cummins, Michael ; Silva, Joana C. ; Mollison, Ewan ; Meikle, Rowan ; Morris, Jenny ; Hedley, Pete E. and Waugh, Robbie , et al. (2019) In Development (Cambridge) 146(11).
Abstract

Many plants dramatically elongate their stems during flowering, yet how this response is coordinated with the reproductive phase is unclear. We demonstrate that microRNA (miRNA) control of APETALA2 (AP2) is required for rapid, complete elongation of stem internodes in barley, especially of the final 'peduncle' internode directly underneath the inflorescence. Disrupted miR172 targeting of AP2 in the Zeo1.b barley mutant caused lower mitotic activity, delayed growth dynamics and premature lignification in the peduncle leading to fewer and shorter cells. Stage- and tissue-specific comparative transcriptomics between Zeo1.b and its parent cultivar showed reduced expression of proliferation-associated genes, ectopic expression of... (More)

Many plants dramatically elongate their stems during flowering, yet how this response is coordinated with the reproductive phase is unclear. We demonstrate that microRNA (miRNA) control of APETALA2 (AP2) is required for rapid, complete elongation of stem internodes in barley, especially of the final 'peduncle' internode directly underneath the inflorescence. Disrupted miR172 targeting of AP2 in the Zeo1.b barley mutant caused lower mitotic activity, delayed growth dynamics and premature lignification in the peduncle leading to fewer and shorter cells. Stage- and tissue-specific comparative transcriptomics between Zeo1.b and its parent cultivar showed reduced expression of proliferation-associated genes, ectopic expression of maturation-related genes and persistent, elevated expression of genes associated with jasmonate and stress responses. We further show that applying methyl jasmonate (MeJA) phenocopied the stem elongation of Zeo1.b, and that Zeo1.b itself was hypersensitive to inhibition by MeJA but less responsive to promotion by gibberellin. Taken together, we propose that miR172-mediated restriction of AP2 may modulate the jasmonate pathway to facilitate gibberellin-promoted stem growth during flowering.

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publication status
published
subject
keywords
APETALA2, Cereal development, Intercalary meristem, Jasmonate, Phase change, Stem elongation
in
Development (Cambridge)
volume
146
issue
11
publisher
The Company of Biologists Ltd
external identifiers
  • pmid:31076487
  • scopus:85068196737
ISSN
1477-9129
DOI
10.1242/dev.170373
language
English
LU publication?
yes
id
0e91c4f1-5aaa-4c1a-b817-771fc913d817
date added to LUP
2019-07-09 15:07:06
date last changed
2020-07-08 04:59:44
@article{0e91c4f1-5aaa-4c1a-b817-771fc913d817,
  abstract     = {<p>Many plants dramatically elongate their stems during flowering, yet how this response is coordinated with the reproductive phase is unclear. We demonstrate that microRNA (miRNA) control of APETALA2 (AP2) is required for rapid, complete elongation of stem internodes in barley, especially of the final 'peduncle' internode directly underneath the inflorescence. Disrupted miR172 targeting of AP2 in the Zeo1.b barley mutant caused lower mitotic activity, delayed growth dynamics and premature lignification in the peduncle leading to fewer and shorter cells. Stage- and tissue-specific comparative transcriptomics between Zeo1.b and its parent cultivar showed reduced expression of proliferation-associated genes, ectopic expression of maturation-related genes and persistent, elevated expression of genes associated with jasmonate and stress responses. We further show that applying methyl jasmonate (MeJA) phenocopied the stem elongation of Zeo1.b, and that Zeo1.b itself was hypersensitive to inhibition by MeJA but less responsive to promotion by gibberellin. Taken together, we propose that miR172-mediated restriction of AP2 may modulate the jasmonate pathway to facilitate gibberellin-promoted stem growth during flowering.</p>},
  author       = {Patil, Vrushali and McDermott, Hannah I. and McAllister, Trisha and Cummins, Michael and Silva, Joana C. and Mollison, Ewan and Meikle, Rowan and Morris, Jenny and Hedley, Pete E. and Waugh, Robbie and Dockter, Christoph and Hansson, Mats and McKim, Sarah M.},
  issn         = {1477-9129},
  language     = {eng},
  month        = {06},
  number       = {11},
  publisher    = {The Company of Biologists Ltd},
  series       = {Development (Cambridge)},
  title        = {APETALA2 control of barley internode elongation},
  url          = {http://dx.doi.org/10.1242/dev.170373},
  doi          = {10.1242/dev.170373},
  volume       = {146},
  year         = {2019},
}