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Islet cell antibodies in insulin-dependent (type 1) diabetic children treated with plasmapheresis

Marner, B. ; Lernmark, A. LU orcid ; Ludvigsson, J. ; MacKay, P. ; Matsuba, I. ; Nerup, J. LU and Rabinovitch, A. (1985) In Diabetes Research 2(5). p.231-236
Abstract

Plasma levels of islet cell cytoplasmic and cytotoxic antibodies were determined in 10 children with insulin-dependent diabetes mellitus (IDDM) treated with plasmapheresis shortly after diagnosis, and in 9 children with IDDM treated by conventional means alone. Islet cell cytoplasmic antibody (ICA) titers were determined by indirect immunofluorescence using unfixed sections of human pancreas, and islet cell cytotoxic antibody levels were determined in a complement-dependent antibody-mediated cytotoxicity (C'AMC) assay using a human fetal cloned insulin-producing cell line (JHPI-1) as target. Before plasmapheresis, ICA was present in 7 out of 10 children and C'AMC was positive in 4. Four successive treatments with plasmapheresis did not... (More)

Plasma levels of islet cell cytoplasmic and cytotoxic antibodies were determined in 10 children with insulin-dependent diabetes mellitus (IDDM) treated with plasmapheresis shortly after diagnosis, and in 9 children with IDDM treated by conventional means alone. Islet cell cytoplasmic antibody (ICA) titers were determined by indirect immunofluorescence using unfixed sections of human pancreas, and islet cell cytotoxic antibody levels were determined in a complement-dependent antibody-mediated cytotoxicity (C'AMC) assay using a human fetal cloned insulin-producing cell line (JHPI-1) as target. Before plasmapheresis, ICA was present in 7 out of 10 children and C'AMC was positive in 4. Four successive treatments with plasmapheresis did not consistently decrease plasma levels of ICA or C'AMC. ICA was present in 15 out of the total 19 children at diagnosis, and titers of ICA decreased in 12 out of 15 subjects by at least 1 degree of dilution (1:3) at 18-30 months follow-up, whether or not they had been treated with plasmapheresis; C'AMC was positive in 6 out of the 18 children at diagnosis and decreased in 2 out of 6. Plasma levels of C-peptide did not differ at diagnosis but remained higher in the plasmapheresis treated diabetic children at 3 and 18-30 months follow-up. Neither ICA titers nor C'AMC levels correlated with plasma C-peptide responses at 18-30 months. It is concluded that plasmapheresis decreases ICA and C'AMC but is followed rapidly by a rebound effect, and does not affect the rates at which these islet cell antibodies decrease with increasing duration off IDDM.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Diabetes Research
volume
2
issue
5
pages
6 pages
publisher
Teviot-Kimpton
external identifiers
  • scopus:0022218219
  • pmid:3905187
ISSN
0265-5985
language
English
LU publication?
no
id
0eab33c5-0d8c-4da7-8a69-b2e03fbd9316
date added to LUP
2019-09-16 12:37:08
date last changed
2024-03-13 08:06:21
@article{0eab33c5-0d8c-4da7-8a69-b2e03fbd9316,
  abstract     = {{<p>Plasma levels of islet cell cytoplasmic and cytotoxic antibodies were determined in 10 children with insulin-dependent diabetes mellitus (IDDM) treated with plasmapheresis shortly after diagnosis, and in 9 children with IDDM treated by conventional means alone. Islet cell cytoplasmic antibody (ICA) titers were determined by indirect immunofluorescence using unfixed sections of human pancreas, and islet cell cytotoxic antibody levels were determined in a complement-dependent antibody-mediated cytotoxicity (C'AMC) assay using a human fetal cloned insulin-producing cell line (JHPI-1) as target. Before plasmapheresis, ICA was present in 7 out of 10 children and C'AMC was positive in 4. Four successive treatments with plasmapheresis did not consistently decrease plasma levels of ICA or C'AMC. ICA was present in 15 out of the total 19 children at diagnosis, and titers of ICA decreased in 12 out of 15 subjects by at least 1 degree of dilution (1:3) at 18-30 months follow-up, whether or not they had been treated with plasmapheresis; C'AMC was positive in 6 out of the 18 children at diagnosis and decreased in 2 out of 6. Plasma levels of C-peptide did not differ at diagnosis but remained higher in the plasmapheresis treated diabetic children at 3 and 18-30 months follow-up. Neither ICA titers nor C'AMC levels correlated with plasma C-peptide responses at 18-30 months. It is concluded that plasmapheresis decreases ICA and C'AMC but is followed rapidly by a rebound effect, and does not affect the rates at which these islet cell antibodies decrease with increasing duration off IDDM.</p>}},
  author       = {{Marner, B. and Lernmark, A. and Ludvigsson, J. and MacKay, P. and Matsuba, I. and Nerup, J. and Rabinovitch, A.}},
  issn         = {{0265-5985}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{5}},
  pages        = {{231--236}},
  publisher    = {{Teviot-Kimpton}},
  series       = {{Diabetes Research}},
  title        = {{Islet cell antibodies in insulin-dependent (type 1) diabetic children treated with plasmapheresis}},
  volume       = {{2}},
  year         = {{1985}},
}