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Regulation of MUC5AC mucin secretion by depletion of AQP5 in SPC-A1 cells

Chen, ZH ; Wang, Xiangdong LU ; Gao, L ; Bai, L ; Zhu, R and Bai, CX (2006) In Biochemical and Biophysical Research Communications 342(3). p.775-781
Abstract
Airway mucus is regulated by many inflammatory mediators such as ILs, TNF-alpha, EGF, PGF2 alpha, LT, and so on. Recently, the relationship between membrane ion channel and Mucus production has been under investigation. The present study aimed to examine whether AQP5 was involved in modulation of mucin expression and secretion in airway submucosal gland cells (SPC-Al). A recombinant plasmid (pShAQP5) containing small hairpin RNA expression cassette targeting AQP5 sequence was constructed. In pShAQP5 transiently transfected cells, ELISA showed MUC5AC synthesis and secretion were increased by 57.9% and 85.3%, respectively, on day 5 after pShAQP5 transfection. While in five stably transfected clones (shAQP5-G1, G2, G3, A2, and A5), the... (More)
Airway mucus is regulated by many inflammatory mediators such as ILs, TNF-alpha, EGF, PGF2 alpha, LT, and so on. Recently, the relationship between membrane ion channel and Mucus production has been under investigation. The present study aimed to examine whether AQP5 was involved in modulation of mucin expression and secretion in airway submucosal gland cells (SPC-Al). A recombinant plasmid (pShAQP5) containing small hairpin RNA expression cassette targeting AQP5 sequence was constructed. In pShAQP5 transiently transfected cells, ELISA showed MUC5AC synthesis and secretion were increased by 57.9% and 85.3%, respectively, on day 5 after pShAQP5 transfection. While in five stably transfected clones (shAQP5-G1, G2, G3, A2, and A5), the upregulated levels of M MAC mRNA were 118%, 165%, 65% 123%, and 38%, respectively. The elevated levels of M MAC synthesis and secretion varied from 59-156% and 33-166%, respectively. This is the first reliable investigation of the regulation of MUC5AC mucin secretion by silencing AQP5. Further study of the regulatory mechanism between AQPs and mucins may provide new strategies for development of novel antihypersecretory drugs in airway diseases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
MUC5AC, RNAi, mucin, AQP5
in
Biochemical and Biophysical Research Communications
volume
342
issue
3
pages
775 - 781
publisher
Elsevier
external identifiers
  • wos:000236168600014
  • pmid:16500622
  • scopus:33644634920
ISSN
1090-2104
DOI
10.1016/j.bbrc.2006.01.103
language
English
LU publication?
yes
id
0ecd84d7-80f4-4a91-9343-c455418e68c1 (old id 415836)
date added to LUP
2016-04-01 15:46:14
date last changed
2020-02-12 07:19:53
@article{0ecd84d7-80f4-4a91-9343-c455418e68c1,
  abstract     = {Airway mucus is regulated by many inflammatory mediators such as ILs, TNF-alpha, EGF, PGF2 alpha, LT, and so on. Recently, the relationship between membrane ion channel and Mucus production has been under investigation. The present study aimed to examine whether AQP5 was involved in modulation of mucin expression and secretion in airway submucosal gland cells (SPC-Al). A recombinant plasmid (pShAQP5) containing small hairpin RNA expression cassette targeting AQP5 sequence was constructed. In pShAQP5 transiently transfected cells, ELISA showed MUC5AC synthesis and secretion were increased by 57.9% and 85.3%, respectively, on day 5 after pShAQP5 transfection. While in five stably transfected clones (shAQP5-G1, G2, G3, A2, and A5), the upregulated levels of M MAC mRNA were 118%, 165%, 65% 123%, and 38%, respectively. The elevated levels of M MAC synthesis and secretion varied from 59-156% and 33-166%, respectively. This is the first reliable investigation of the regulation of MUC5AC mucin secretion by silencing AQP5. Further study of the regulatory mechanism between AQPs and mucins may provide new strategies for development of novel antihypersecretory drugs in airway diseases.},
  author       = {Chen, ZH and Wang, Xiangdong and Gao, L and Bai, L and Zhu, R and Bai, CX},
  issn         = {1090-2104},
  language     = {eng},
  number       = {3},
  pages        = {775--781},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Regulation of MUC5AC mucin secretion by depletion of AQP5 in SPC-A1 cells},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2006.01.103},
  doi          = {10.1016/j.bbrc.2006.01.103},
  volume       = {342},
  year         = {2006},
}