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Characterization of humoral responses to soluble trimeric HIV gp140 from a clade A Ugandan field isolate

Visciano, Maria Luisa ; Tagliamonte, Maria ; Stewart-Jones, Guillaume ; Heyndrickx, Leo ; Vanham, Guido ; Jansson, Marianne LU ; Fomsgaard, Anders ; Grevstad, Berit ; Ramaswamy, Meghna and Buonaguro, Franco M. , et al. (2013) In Journal of Translational Medicine 11(165).
Abstract
Trimeric soluble forms of HIV gp140 envelope glycoproteins represent one of the closest molecular structures compared to native spikes present on intact virus particles. Trimeric soluble gp140 have been generated by several groups and such molecules have been shown to induce antibodies with neutralizing activity against homologous and heterologous viruses. In the present study, we generated a recombinant trimeric soluble gp140, derived from a previously identified Ugandan A-clade HIV field isolate (gp140(94UG018)). Antibodies elicited in immunized rabbits show a broad binding pattern to HIV envelopes of different clades. An epitope mapping analysis reveals that, on average, the binding is mostly focused on the C1, C2, V3, V5 and C5... (More)
Trimeric soluble forms of HIV gp140 envelope glycoproteins represent one of the closest molecular structures compared to native spikes present on intact virus particles. Trimeric soluble gp140 have been generated by several groups and such molecules have been shown to induce antibodies with neutralizing activity against homologous and heterologous viruses. In the present study, we generated a recombinant trimeric soluble gp140, derived from a previously identified Ugandan A-clade HIV field isolate (gp140(94UG018)). Antibodies elicited in immunized rabbits show a broad binding pattern to HIV envelopes of different clades. An epitope mapping analysis reveals that, on average, the binding is mostly focused on the C1, C2, V3, V5 and C5 regions. Immune sera show neutralization activity to Tier 1 isolates of different clades, demonstrating cross clade neutralizing activity which needs to be further broadened by possible structural modifications of the clade A gp140(94UG018). Our results provide a rationale for the design and evaluation of immunogens and the clade A gp140(94UG018) shows promising characteristics for potential involvement in an effective HIV vaccine with broad activity. (Less)
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Journal of Translational Medicine
volume
11
issue
165
publisher
BioMed Central
external identifiers
  • wos:000322528500001
  • scopus:84879833545
  • pmid:23835244
ISSN
1479-5876
DOI
10.1186/1479-5876-11-165
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English
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yes
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0ef4ef45-5506-4d99-aefb-cf112f03a0f2 (old id 4053364)
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2016-04-01 15:01:51
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@article{0ef4ef45-5506-4d99-aefb-cf112f03a0f2,
  abstract     = {Trimeric soluble forms of HIV gp140 envelope glycoproteins represent one of the closest molecular structures compared to native spikes present on intact virus particles. Trimeric soluble gp140 have been generated by several groups and such molecules have been shown to induce antibodies with neutralizing activity against homologous and heterologous viruses. In the present study, we generated a recombinant trimeric soluble gp140, derived from a previously identified Ugandan A-clade HIV field isolate (gp140(94UG018)). Antibodies elicited in immunized rabbits show a broad binding pattern to HIV envelopes of different clades. An epitope mapping analysis reveals that, on average, the binding is mostly focused on the C1, C2, V3, V5 and C5 regions. Immune sera show neutralization activity to Tier 1 isolates of different clades, demonstrating cross clade neutralizing activity which needs to be further broadened by possible structural modifications of the clade A gp140(94UG018). Our results provide a rationale for the design and evaluation of immunogens and the clade A gp140(94UG018) shows promising characteristics for potential involvement in an effective HIV vaccine with broad activity.},
  author       = {Visciano, Maria Luisa and Tagliamonte, Maria and Stewart-Jones, Guillaume and Heyndrickx, Leo and Vanham, Guido and Jansson, Marianne and Fomsgaard, Anders and Grevstad, Berit and Ramaswamy, Meghna and Buonaguro, Franco M. and Tornesello, Maria Lina and Biswas, Priscilla and Scarlatti, Gabriella and Buonaguro, Luigi},
  issn         = {1479-5876},
  language     = {eng},
  number       = {165},
  publisher    = {BioMed Central},
  series       = {Journal of Translational Medicine},
  title        = {Characterization of humoral responses to soluble trimeric HIV gp140 from a clade A Ugandan field isolate},
  url          = {https://lup.lub.lu.se/search/ws/files/4306035/4281441.pdf},
  doi          = {10.1186/1479-5876-11-165},
  volume       = {11},
  year         = {2013},
}