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Glucose and xylose co-fermentation of pretreated wheat straw using mutants of S. cerevisiae TMB3400.

Erdei, Borbala LU ; Franko, Balazs LU ; Galbe, Mats LU and Zacchi, Guido LU (2013) In Journal of Biotechnology 164(1). p.50-58
Abstract
Wheat straw was pretreated and fermented to ethanol. Two strains, which had been mutated from the genetically modified Saccharomyces cerevisiae TMB3400, KE6-12 and KE6-13i, have been used in this study and the results of performance were compared to that of the original strain. The glucose and xylose co-fermentation ability was investigated in batch fermentation of steam-pretreated wheat straw (SPWS) liquid (undiluted, and diluted 1.5 and 2 times). Both strains showed improved xylose uptake in diluted SPWS liquid, and increased ethanol yields compared with the original TMB3400 strain, although xylitol formation also increased slightly. In undiluted SPWS liquid, however, only KE6-13i performed better than the original strain regarding... (More)
Wheat straw was pretreated and fermented to ethanol. Two strains, which had been mutated from the genetically modified Saccharomyces cerevisiae TMB3400, KE6-12 and KE6-13i, have been used in this study and the results of performance were compared to that of the original strain. The glucose and xylose co-fermentation ability was investigated in batch fermentation of steam-pretreated wheat straw (SPWS) liquid (undiluted, and diluted 1.5 and 2 times). Both strains showed improved xylose uptake in diluted SPWS liquid, and increased ethanol yields compared with the original TMB3400 strain, although xylitol formation also increased slightly. In undiluted SPWS liquid, however, only KE6-13i performed better than the original strain regarding xylose utilization. Fed-batch fermentation of 1.5 and 2 times diluted liquid was performed by adding the glucose-rich hydrolysates from enzymatic hydrolysis of the solid fraction of SPWS at a constant feed rate after 5h of fermentation, when the glucose had been depleted. The modified strains showed improved xylose conversion; however, the ethanol yield was not significantly improved due to increased glycerol production. Fed-batch fermentation resulted in faster xylose utilization than in the batch cases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
164
issue
1
pages
50 - 58
publisher
Elsevier
external identifiers
  • wos:000315527900008
  • pmid:23262129
  • scopus:84875106231
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2012.12.003
language
English
LU publication?
yes
id
0f5c59ba-dbfc-45c2-8932-0db1fa07fc1d (old id 3346943)
date added to LUP
2013-01-07 12:41:51
date last changed
2019-01-06 03:29:30
@article{0f5c59ba-dbfc-45c2-8932-0db1fa07fc1d,
  abstract     = {Wheat straw was pretreated and fermented to ethanol. Two strains, which had been mutated from the genetically modified Saccharomyces cerevisiae TMB3400, KE6-12 and KE6-13i, have been used in this study and the results of performance were compared to that of the original strain. The glucose and xylose co-fermentation ability was investigated in batch fermentation of steam-pretreated wheat straw (SPWS) liquid (undiluted, and diluted 1.5 and 2 times). Both strains showed improved xylose uptake in diluted SPWS liquid, and increased ethanol yields compared with the original TMB3400 strain, although xylitol formation also increased slightly. In undiluted SPWS liquid, however, only KE6-13i performed better than the original strain regarding xylose utilization. Fed-batch fermentation of 1.5 and 2 times diluted liquid was performed by adding the glucose-rich hydrolysates from enzymatic hydrolysis of the solid fraction of SPWS at a constant feed rate after 5h of fermentation, when the glucose had been depleted. The modified strains showed improved xylose conversion; however, the ethanol yield was not significantly improved due to increased glycerol production. Fed-batch fermentation resulted in faster xylose utilization than in the batch cases.},
  author       = {Erdei, Borbala and Franko, Balazs and Galbe, Mats and Zacchi, Guido},
  issn         = {1873-4863},
  language     = {eng},
  number       = {1},
  pages        = {50--58},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Glucose and xylose co-fermentation of pretreated wheat straw using mutants of S. cerevisiae TMB3400.},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2012.12.003},
  volume       = {164},
  year         = {2013},
}