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The lower expression of parvalbumin in the primary somatosensory cortex of WAG/Rij rats may facilitate the occurrence of absence seizures

Arkan, Sertan LU ; Kasap, Murat ; Akman, Özlem ; Akpınar, Gürler ; Ateş, Nurbay and Karson, Ayşe (2019) In Neuroscience Letters 709.
Abstract

Absence epilepsy (AE) is classified as a genetic generalized epilepsies. WAG/Rij strain of rats are regarded one of the most validated models of absence epilepsy. Studies point out the existence of hyperexcitable focus in somatosensory cortex of these rats, which has been attributed to the deficits in the GABAergic system. In the current study, we studied the changes of calcium binding proteins (CaBPs) in somatosensory cortex (S1) of the 2 and 8 month-old WAG/Rij rats and their age-matched Wistar Albino controls by investigating the expression levels of CaBPs (calbindin, calretinin and parvalbumin) in western blotting. Since WAG/Rij rats showed the low expression level of parvalbumin (PV) in western blots in comparison to Wistar Albino... (More)

Absence epilepsy (AE) is classified as a genetic generalized epilepsies. WAG/Rij strain of rats are regarded one of the most validated models of absence epilepsy. Studies point out the existence of hyperexcitable focus in somatosensory cortex of these rats, which has been attributed to the deficits in the GABAergic system. In the current study, we studied the changes of calcium binding proteins (CaBPs) in somatosensory cortex (S1) of the 2 and 8 month-old WAG/Rij rats and their age-matched Wistar Albino controls by investigating the expression levels of CaBPs (calbindin, calretinin and parvalbumin) in western blotting. Since WAG/Rij rats showed the low expression level of parvalbumin (PV) in western blots in comparison to Wistar Albino rats, we selectively investigated the number of PV positive neurons using the immunofluorescence staining method in order to confirm this decrement in the perioral region of somatosensory cortex (S1po). The most critical finding of this study was the age- independent reduction in the expression level of PV in the somatosensory cortex of epileptic rats as demonstrating western blotting. Nevertheless, no significant difference was found among numbers of PV + neuron in the S1po region by immunofluorescence staining concerning both of age and strain dependency. These results suggest that the disruption in the activity of the PV-expressing GABAergic interneurons might be involved in the generation of rather than the age-dependent increase in the SWDs in WAG/Rij rats.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Absence epilepsy, Calbindin, Calcium binding proteins, Calretinin, Parvalbumin, WAG/Rij
in
Neuroscience Letters
volume
709
article number
134299
publisher
Elsevier
external identifiers
  • pmid:31153971
  • scopus:85067272583
ISSN
0304-3940
DOI
10.1016/j.neulet.2019.134299
language
English
LU publication?
yes
id
0f6d1c67-29dc-48f5-bc12-4893e730273f
date added to LUP
2019-06-26 10:49:08
date last changed
2019-07-30 05:04:54
@article{0f6d1c67-29dc-48f5-bc12-4893e730273f,
  abstract     = {<p>Absence epilepsy (AE) is classified as a genetic generalized epilepsies. WAG/Rij strain of rats are regarded one of the most validated models of absence epilepsy. Studies point out the existence of hyperexcitable focus in somatosensory cortex of these rats, which has been attributed to the deficits in the GABAergic system. In the current study, we studied the changes of calcium binding proteins (CaBPs) in somatosensory cortex (S1) of the 2 and 8 month-old WAG/Rij rats and their age-matched Wistar Albino controls by investigating the expression levels of CaBPs (calbindin, calretinin and parvalbumin) in western blotting. Since WAG/Rij rats showed the low expression level of parvalbumin (PV) in western blots in comparison to Wistar Albino rats, we selectively investigated the number of PV positive neurons using the immunofluorescence staining method in order to confirm this decrement in the perioral region of somatosensory cortex (S1po). The most critical finding of this study was the age- independent reduction in the expression level of PV in the somatosensory cortex of epileptic rats as demonstrating western blotting. Nevertheless, no significant difference was found among numbers of PV + neuron in the S1po region by immunofluorescence staining concerning both of age and strain dependency. These results suggest that the disruption in the activity of the PV-expressing GABAergic interneurons might be involved in the generation of rather than the age-dependent increase in the SWDs in WAG/Rij rats.</p>},
  author       = {Arkan, Sertan and Kasap, Murat and Akman, Özlem and Akpınar, Gürler and Ateş, Nurbay and Karson, Ayşe},
  issn         = {0304-3940},
  language     = {eng},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {The lower expression of parvalbumin in the primary somatosensory cortex of WAG/Rij rats may facilitate the occurrence of absence seizures},
  url          = {http://dx.doi.org/10.1016/j.neulet.2019.134299},
  doi          = {10.1016/j.neulet.2019.134299},
  volume       = {709},
  year         = {2019},
}