Chromatography in Downstream Processing of Recombinant Adeno-Associated Viruses : A Review of Current and Future Practises
Lorek, Julius Klemens LU
; Isaksson, Madelène
LU
and Nilsson, Bernt
LU
(2025)
In Biotechnology and Bioengineering
- Abstract
Recombinant adeno-associated virus (rAAV) has emerged as an attractive gene delivery vector platform to treat both rare and pervasive diseases. With more and more rAAV-based therapies entering late-stage clinical trials and commercialization, there is an increasing pressure on the rAAV manufacturing process to accelerate drug development, account for larger trials, and commercially provide high doses. Still, many of the pre-clinical and clinical manufacturing processes are tied to outdated technologies, which results in substantial production expenses. Those processes face challenges including low productivity and difficult scalability, which limits its ability to provide for required dosages which in turn influences the accessibility... (More)
Recombinant adeno-associated virus (rAAV) has emerged as an attractive gene delivery vector platform to treat both rare and pervasive diseases. With more and more rAAV-based therapies entering late-stage clinical trials and commercialization, there is an increasing pressure on the rAAV manufacturing process to accelerate drug development, account for larger trials, and commercially provide high doses. Still, many of the pre-clinical and clinical manufacturing processes are tied to outdated technologies, which results in substantial production expenses. Those processes face challenges including low productivity and difficult scalability, which limits its ability to provide for required dosages which in turn influences the accessibility of the drug. And as upstream efforts are expected to increase productivities, the downstream part needs to adapt with more scalable and efficient technologies. In this review, both traditional and novel rAAV downstream technologies are presented and discussed. Traditional rAAV downstream processes are based on density gradient ultracentrifugation and have been shown to effectively purify rAAVs with high yields and purities. However, those processes lack scalability and efficiency, which is why novel rAAV downstream processes based on column-chromatography have emerged as an attractive alternative and show potential for integration in continuous processes, following the principle of next-generation manufacturing.
(Less)
- author
- Lorek, Julius Klemens
LU
; Isaksson, Madelène
LU
and Nilsson, Bernt
LU
- organization
- publishing date
- 2025
- type
- Contribution to journal
- publication status
- epub
- subject
- keywords
- column-chromatography, continuous biomanufacturing, gene therapy, rAAV downstream processing, rAAV manufacturing, recombinant Adeno-associated virus
- in
- Biotechnology and Bioengineering
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85216854014
- pmid:39905691
- ISSN
- 0006-3592
- DOI
- 10.1002/bit.28932
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2025 The Author(s). Biotechnology and Bioengineering published by Wiley Periodicals LLC.
- id
- 0f75a13e-0741-49be-b12f-7bf4f086b3a2
- date added to LUP
- 2025-04-11 13:37:09
- date last changed
- 2025-07-04 20:33:55
@article{0f75a13e-0741-49be-b12f-7bf4f086b3a2,
abstract = {{<p>Recombinant adeno-associated virus (rAAV) has emerged as an attractive gene delivery vector platform to treat both rare and pervasive diseases. With more and more rAAV-based therapies entering late-stage clinical trials and commercialization, there is an increasing pressure on the rAAV manufacturing process to accelerate drug development, account for larger trials, and commercially provide high doses. Still, many of the pre-clinical and clinical manufacturing processes are tied to outdated technologies, which results in substantial production expenses. Those processes face challenges including low productivity and difficult scalability, which limits its ability to provide for required dosages which in turn influences the accessibility of the drug. And as upstream efforts are expected to increase productivities, the downstream part needs to adapt with more scalable and efficient technologies. In this review, both traditional and novel rAAV downstream technologies are presented and discussed. Traditional rAAV downstream processes are based on density gradient ultracentrifugation and have been shown to effectively purify rAAVs with high yields and purities. However, those processes lack scalability and efficiency, which is why novel rAAV downstream processes based on column-chromatography have emerged as an attractive alternative and show potential for integration in continuous processes, following the principle of next-generation manufacturing.</p>}},
author = {{Lorek, Julius Klemens and Isaksson, Madelène and Nilsson, Bernt}},
issn = {{0006-3592}},
keywords = {{column-chromatography; continuous biomanufacturing; gene therapy; rAAV downstream processing; rAAV manufacturing; recombinant Adeno-associated virus}},
language = {{eng}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Chromatography in Downstream Processing of Recombinant Adeno-Associated Viruses : A Review of Current and Future Practises}},
url = {{http://dx.doi.org/10.1002/bit.28932}},
doi = {{10.1002/bit.28932}},
year = {{2025}},
}