Surface-modified nanoerythrosomes for potential optical imaging diagnostics
Fornasier, Marco ; Porcheddu, Andrea ; Casu, Anna ; Raghavan, Srinivasa R. ; Jönsson, Peter LU ; Schillén, Karin LU
and Murgia, Sergio
(2021)
In Journal of Colloid and Interface Science
582(Part A).
p.246-253
- Abstract
Nanoerythrosomes (NERs), vesicle-like nanoparticles derived from red blood cells, represent a new and interesting vector for therapeutic molecules and imaging probes, mainly thanks to their high stability and excellent biocompatibility. Aiming to present a proof-of-concept of the use of NERs as diagnostic tools for in vitro/in vivo imaging purposes, we report here several functionalization routes to decorate the surfaces of NERs derived from bovine blood with two different fluorophores: 7-amino-4-methylcumarin and dibenzocyclooctinecyanine5.5. Notably, the fluorophores were cross-linked to the NERs surface with glutaraldehyde and, in the case of dibenzocyclooctinecyanine5.5, also using a click-chemistry route, termed strain-promoted... (More)
Nanoerythrosomes (NERs), vesicle-like nanoparticles derived from red blood cells, represent a new and interesting vector for therapeutic molecules and imaging probes, mainly thanks to their high stability and excellent biocompatibility. Aiming to present a proof-of-concept of the use of NERs as diagnostic tools for in vitro/in vivo imaging purposes, we report here several functionalization routes to decorate the surfaces of NERs derived from bovine blood with two different fluorophores: 7-amino-4-methylcumarin and dibenzocyclooctinecyanine5.5. Notably, the fluorophores were cross-linked to the NERs surface with glutaraldehyde and, in the case of dibenzocyclooctinecyanine5.5, also using a click-chemistry route, termed strain-promoted azide-alkyne cycloaddition. The physicochemical characterization highlighted the high stability of the NERs derivatives in physiological conditions. Furthermore, the loading efficiency of the fluorophores on the NERs surface was evaluated using both UV–Vis spectroscopy and fluorescence microscopy.
(Less)
- author
- Fornasier, Marco
; Porcheddu, Andrea
; Casu, Anna
; Raghavan, Srinivasa R.
; Jönsson, Peter
LU
; Schillén, Karin
LU
and Murgia, Sergio
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cu-free click chemistry, Fluorescence, Ghosts, Red blood cells, Vesicles
- in
- Journal of Colloid and Interface Science
- volume
- 582
- issue
- Part A
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85089517458
- pmid:32823126
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2020.08.032
- language
- English
- LU publication?
- yes
- id
- 0f95cdaf-c5ce-479c-9823-72dead3dc924
- date added to LUP
- 2020-08-27 09:18:05
- date last changed
- 2024-06-12 19:33:33
@article{0f95cdaf-c5ce-479c-9823-72dead3dc924,
abstract = {{<p>Nanoerythrosomes (NERs), vesicle-like nanoparticles derived from red blood cells, represent a new and interesting vector for therapeutic molecules and imaging probes, mainly thanks to their high stability and excellent biocompatibility. Aiming to present a proof-of-concept of the use of NERs as diagnostic tools for in vitro/in vivo imaging purposes, we report here several functionalization routes to decorate the surfaces of NERs derived from bovine blood with two different fluorophores: 7-amino-4-methylcumarin and dibenzocyclooctinecyanine5.5. Notably, the fluorophores were cross-linked to the NERs surface with glutaraldehyde and, in the case of dibenzocyclooctinecyanine5.5, also using a click-chemistry route, termed strain-promoted azide-alkyne cycloaddition. The physicochemical characterization highlighted the high stability of the NERs derivatives in physiological conditions. Furthermore, the loading efficiency of the fluorophores on the NERs surface was evaluated using both UV–Vis spectroscopy and fluorescence microscopy.</p>}},
author = {{Fornasier, Marco and Porcheddu, Andrea and Casu, Anna and Raghavan, Srinivasa R. and Jönsson, Peter and Schillén, Karin and Murgia, Sergio}},
issn = {{0021-9797}},
keywords = {{Cu-free click chemistry; Fluorescence; Ghosts; Red blood cells; Vesicles}},
language = {{eng}},
number = {{Part A}},
pages = {{246--253}},
publisher = {{Elsevier}},
series = {{Journal of Colloid and Interface Science}},
title = {{Surface-modified nanoerythrosomes for potential optical imaging diagnostics}},
url = {{http://dx.doi.org/10.1016/j.jcis.2020.08.032}},
doi = {{10.1016/j.jcis.2020.08.032}},
volume = {{582}},
year = {{2021}},
}