Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients
(2022) In Methods in Molecular Biology 2363. p.1-12- Abstract
The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0fbb69ce-4d4c-4b39-be47-db0bf3642f11
- author
- Tran, Huy Cuong LU and Van Aken, Olivier LU
- organization
- publishing date
- 2022
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Arabidopsis, Density gradients, Mitochondria, Mitochondrial isolation
- host publication
- Plant Mitochondria : Methods and Protocols - Methods and Protocols
- series title
- Methods in Molecular Biology
- editor
- Van Aken, Olivier and Rasmusson, Allan G.
- volume
- 2363
- pages
- 12 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:34545481
- scopus:85115826347
- ISSN
- 1940-6029
- 1064-3745
- ISBN
- 978-1-0716-1653-6
- 978-1-0716-1652-9
- DOI
- 10.1007/978-1-0716-1653-6_1
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
- id
- 0fbb69ce-4d4c-4b39-be47-db0bf3642f11
- date added to LUP
- 2021-11-17 15:53:32
- date last changed
- 2024-09-22 05:37:51
@inbook{0fbb69ce-4d4c-4b39-be47-db0bf3642f11, abstract = {{<p>The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.</p>}}, author = {{Tran, Huy Cuong and Van Aken, Olivier}}, booktitle = {{Plant Mitochondria : Methods and Protocols}}, editor = {{Van Aken, Olivier and Rasmusson, Allan G.}}, isbn = {{978-1-0716-1653-6}}, issn = {{1940-6029}}, keywords = {{Arabidopsis; Density gradients; Mitochondria; Mitochondrial isolation}}, language = {{eng}}, pages = {{1--12}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients}}, url = {{http://dx.doi.org/10.1007/978-1-0716-1653-6_1}}, doi = {{10.1007/978-1-0716-1653-6_1}}, volume = {{2363}}, year = {{2022}}, }