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Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Tran, Huy Cuong LU orcid and Van Aken, Olivier LU (2022) In Methods in Molecular Biology 2363. p.1-12
Abstract

The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.

Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Arabidopsis, Density gradients, Mitochondria, Mitochondrial isolation
host publication
Plant Mitochondria : Methods and Protocols - Methods and Protocols
series title
Methods in Molecular Biology
editor
Van Aken, Olivier and Rasmusson, Allan G.
volume
2363
pages
12 pages
publisher
Humana Press
external identifiers
  • pmid:34545481
  • scopus:85115826347
ISSN
1064-3745
1940-6029
ISBN
978-1-0716-1653-6
978-1-0716-1652-9
DOI
10.1007/978-1-0716-1653-6_1
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
id
0fbb69ce-4d4c-4b39-be47-db0bf3642f11
date added to LUP
2021-11-17 15:53:32
date last changed
2024-06-15 20:38:21
@inbook{0fbb69ce-4d4c-4b39-be47-db0bf3642f11,
  abstract     = {{<p>The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.</p>}},
  author       = {{Tran, Huy Cuong and Van Aken, Olivier}},
  booktitle    = {{Plant Mitochondria : Methods and Protocols}},
  editor       = {{Van Aken, Olivier and Rasmusson, Allan G.}},
  isbn         = {{978-1-0716-1653-6}},
  issn         = {{1064-3745}},
  keywords     = {{Arabidopsis; Density gradients; Mitochondria; Mitochondrial isolation}},
  language     = {{eng}},
  pages        = {{1--12}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-1653-6_1}},
  doi          = {{10.1007/978-1-0716-1653-6_1}},
  volume       = {{2363}},
  year         = {{2022}},
}