Protein crystallization benefits from the rough well surface of a 48-well polystyrene microplate
(2019) In Journal of Crystal Growth 532. p.125425-125425- Abstract
- It’s not clear whether the drop well surface can induce heterogeneous nucleation to increase the probability of screening success, although a medium-throughput 48-well SBS (the Society for Biomolecular Screening, USA) polystyrene microplate has been widely used for protein crystallization screening. Here, our accumulating data suggested that this 48-well SBS microplate with rough surface is beneficial for protein crystallization. It was observed that some protein crystals were only formed on the rough edges and/or walls of the drop well, but not on the flat bottom or in the crystallization drop. It was further demonstrated with two standard test proteins. The protuberances, grooves and cavities with varied sizes and shapes at... (More)
- It’s not clear whether the drop well surface can induce heterogeneous nucleation to increase the probability of screening success, although a medium-throughput 48-well SBS (the Society for Biomolecular Screening, USA) polystyrene microplate has been widely used for protein crystallization screening. Here, our accumulating data suggested that this 48-well SBS microplate with rough surface is beneficial for protein crystallization. It was observed that some protein crystals were only formed on the rough edges and/or walls of the drop well, but not on the flat bottom or in the crystallization drop. It was further demonstrated with two standard test proteins. The protuberances, grooves and cavities with varied sizes and shapes at micrometer-scale in the rough drop well wall can serve as nucleants and induce heterogeneous nucleation of protein crystals. The rough wall microplate provides a potent tool for protein crystallographers embarking on crystallization trials. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0fc8d6e2-4944-46ef-821e-6f1e1a482961
- author
- Ye, Zhao-yang ; Broströmer, Erik LU ; Nan, Jie LU ; Cao, Xiao-fang ; Liu, Xiang ; Qiao, Li ; Liu, Zhong-jing and Su, Xiao-dong LU
- publishing date
- 2019-12-13
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Crystal Growth
- volume
- 532
- pages
- 125425 - 125425
- publisher
- Elsevier
- external identifiers
-
- scopus:85076685506
- ISSN
- 0022-0248
- DOI
- 10.1016/j.jcrysgro.2019.125425
- language
- English
- LU publication?
- no
- id
- 0fc8d6e2-4944-46ef-821e-6f1e1a482961
- alternative location
- https://linkinghub.elsevier.com/retrieve/pii/S0022024819306402
- date added to LUP
- 2020-02-13 16:42:37
- date last changed
- 2022-04-18 20:36:49
@article{0fc8d6e2-4944-46ef-821e-6f1e1a482961, abstract = {{It’s not clear whether the drop well surface can induce heterogeneous nucleation to increase the probability of screening success, although a medium-throughput 48-well SBS (the Society for Biomolecular Screening, USA) polystyrene microplate has been widely used for protein crystallization screening. Here, our accumulating data suggested that this 48-well SBS microplate with rough surface is beneficial for protein crystallization. It was observed that some protein crystals were only formed on the rough edges and/or walls of the drop well, but not on the flat bottom or in the crystallization drop. It was further demonstrated with two standard test proteins. The protuberances, grooves and cavities with varied sizes and shapes at micrometer-scale in the rough drop well wall can serve as nucleants and induce heterogeneous nucleation of protein crystals. The rough wall microplate provides a potent tool for protein crystallographers embarking on crystallization trials.}}, author = {{Ye, Zhao-yang and Broströmer, Erik and Nan, Jie and Cao, Xiao-fang and Liu, Xiang and Qiao, Li and Liu, Zhong-jing and Su, Xiao-dong}}, issn = {{0022-0248}}, language = {{eng}}, month = {{12}}, pages = {{125425--125425}}, publisher = {{Elsevier}}, series = {{Journal of Crystal Growth}}, title = {{Protein crystallization benefits from the rough well surface of a 48-well polystyrene microplate}}, url = {{http://dx.doi.org/10.1016/j.jcrysgro.2019.125425}}, doi = {{10.1016/j.jcrysgro.2019.125425}}, volume = {{532}}, year = {{2019}}, }