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Propionic acid production from glycerol in immobilized cell bioreactor using an acid-tolerant strain of Propionibacterium acidipropionici obtained by adaptive evolution

Cavero-Olguin, Victor Hugo LU ; Rahimpour, Farshad LU ; Dishisha, Tarek LU ; Alvarez-Aliaga, Maria Teresa and Hatti-Kaul, Rajni LU (2021) In Process Biochemistry 110. p.223-230
Abstract

Propionic acid (PA) production from agro-industrial residues using propionibacteria has gained interest as an alternative to fossil-based process. Microbial production is however characterized by product inhibition, lowering the product titers and productivity. In this study, Propionibacterium acidipropionici DSMZ4900 was subjected to adaptive evolution to tolerate higher acid concentrations. The strain adapted to growth in medium spiked with 20 g/L PA exhibited improved product titer (16.8 vs 8.72 g/L) and productivity (0.52 vs 0.17 g/L·h) with glycerol as carbon source in batch fermentations. It was immobilized on polyethyleneimine coated recycled glass beads Poraver® and used for fermentations in recycle batch mode with... (More)

Propionic acid (PA) production from agro-industrial residues using propionibacteria has gained interest as an alternative to fossil-based process. Microbial production is however characterized by product inhibition, lowering the product titers and productivity. In this study, Propionibacterium acidipropionici DSMZ4900 was subjected to adaptive evolution to tolerate higher acid concentrations. The strain adapted to growth in medium spiked with 20 g/L PA exhibited improved product titer (16.8 vs 8.72 g/L) and productivity (0.52 vs 0.17 g/L·h) with glycerol as carbon source in batch fermentations. It was immobilized on polyethyleneimine coated recycled glass beads Poraver® and used for fermentations in recycle batch mode with increasing glycerol concentration and decreasing pH, respectively. Doubling yeast extract concentration raised PA yield and productivity by >1.5 fold. Glycerol at 100 g/L was completely consumed to give ∼58 g/L PA at yield of 0.64 mol/mol and productivity of 0.28 g/L·h at pH 6.5. Decreasing fermentation pH to 5.0 increased PA productivity to 0.23 g/L·h from 0.14 g/L·h at pH 6.0 with 20 g/L glycerol, while immobilized cells exhibited no growth. The study shows combination of adaptive evolution and immobilization of cells to result in a robust system for PA fermentation at high glycerol concentration and lower pH.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adaptive evolution, Cell immobilization, Packed bed reactor, Propionibacterium acidipropionici, Propionic acid
in
Process Biochemistry
volume
110
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85113715558
ISSN
1359-5113
DOI
10.1016/j.procbio.2021.08.005
language
English
LU publication?
yes
id
0fdc1bda-2283-4413-82ab-30f84566c5aa
date added to LUP
2021-09-08 10:12:48
date last changed
2022-04-27 03:47:49
@article{0fdc1bda-2283-4413-82ab-30f84566c5aa,
  abstract     = {{<p>Propionic acid (PA) production from agro-industrial residues using propionibacteria has gained interest as an alternative to fossil-based process. Microbial production is however characterized by product inhibition, lowering the product titers and productivity. In this study, <i>Propionibacterium acidipropionici</i> DSMZ4900 was subjected to adaptive evolution to tolerate higher acid concentrations. The strain adapted to growth in medium spiked with 20 g/L PA exhibited improved product titer (16.8 vs 8.72 g/L) and productivity (0.52 vs 0.17 g/L·h) with glycerol as carbon source in batch fermentations. It was immobilized on polyethyleneimine coated recycled glass beads Poraver® and used for fermentations in recycle batch mode with increasing glycerol concentration and decreasing pH, respectively. Doubling yeast extract concentration raised PA yield and productivity by &gt;1.5 fold. Glycerol at 100 g/L was completely consumed to give ∼58 g/L PA at yield of 0.64 mol/mol and productivity of 0.28 g/L·h at pH 6.5. Decreasing fermentation pH to 5.0 increased PA productivity to 0.23 g/L·h from 0.14 g/L·h at pH 6.0 with 20 g/L glycerol, while immobilized cells exhibited no growth. The study shows combination of adaptive evolution and immobilization of cells to result in a robust system for PA fermentation at high glycerol concentration and lower pH.</p>}},
  author       = {{Cavero-Olguin, Victor Hugo and Rahimpour, Farshad and Dishisha, Tarek and Alvarez-Aliaga, Maria Teresa and Hatti-Kaul, Rajni}},
  issn         = {{1359-5113}},
  keywords     = {{Adaptive evolution; Cell immobilization; Packed bed reactor; Propionibacterium acidipropionici; Propionic acid}},
  language     = {{eng}},
  pages        = {{223--230}},
  publisher    = {{Elsevier}},
  series       = {{Process Biochemistry}},
  title        = {{Propionic acid production from glycerol in immobilized cell bioreactor using an acid-tolerant strain of <i>Propionibacterium acidipropionici</i> obtained by adaptive evolution}},
  url          = {{http://dx.doi.org/10.1016/j.procbio.2021.08.005}},
  doi          = {{10.1016/j.procbio.2021.08.005}},
  volume       = {{110}},
  year         = {{2021}},
}