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Standardisation and use of the alcohol biomarker carbohydrate-deficient transferrin (CDT)

Helander, Anders; Wielders, Jos; Anton, Raymond; Arndt, Torsten; Bianchi, Vincenza; Deenmamode, Jean; Jeppsson, Jan-Olof LU ; Whitfield, John B.; Weykamp, Cas and Schellenberg, François (2016) In Clinica Chimica Acta 459. p.19-24
Abstract

Carbohydrate-deficient transferrin (CDT) is a glycoform profile of serum transferrin that increases in response to sustained high alcohol intake and over the last decades has become an important alcohol biomarker with clinical and forensic applications. However, the wide range of CDT measurement procedures has resulted in lack of uniform results and reference limits, and hampered comparison of results. In 2005, the IFCC therefore founded a special working group (WG) aiming for standardisation of CDT measurement. This review summarises the history of CDT and the actions taken by the WG-CDT. Initial steps included the definition of the measurand (serum disialotransferrin to total transferrin fraction expressed in %), and the determination... (More)

Carbohydrate-deficient transferrin (CDT) is a glycoform profile of serum transferrin that increases in response to sustained high alcohol intake and over the last decades has become an important alcohol biomarker with clinical and forensic applications. However, the wide range of CDT measurement procedures has resulted in lack of uniform results and reference limits, and hampered comparison of results. In 2005, the IFCC therefore founded a special working group (WG) aiming for standardisation of CDT measurement. This review summarises the history of CDT and the actions taken by the WG-CDT. Initial steps included the definition of the measurand (serum disialotransferrin to total transferrin fraction expressed in %), and the determination of a well-defined anion-exchange HPLC procedure as the candidate reference measurement procedure (cRMP). Subsequent achievements were the establishment of a network of reference laboratories to perform the cRMP, setting a reference interval, and development of a reference material based on human serum for which the laboratory network assign values. Using a set of reference materials for calibration allowed for achieving equivalence of results of all present CDT measurement procedures. The final steps of the WG-CDT have been a full validation of the cRMP to make it an IFCC approved RMP, and providing guidance for international standardisation of all CDT measurement procedures.

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Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcohol biomarker, Carbohydrate-deficient transferrin, CDT, HPLC, IFCC, Serum transferrin, Standardisation
in
Clinica Chimica Acta
volume
459
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:84969819975
  • wos:000380869500004
ISSN
0009-8981
DOI
10.1016/j.cca.2016.05.016
language
English
LU publication?
no
id
10210819-eba9-4edb-9732-77c892c2cad1
date added to LUP
2016-06-16 14:16:19
date last changed
2017-06-11 05:06:22
@article{10210819-eba9-4edb-9732-77c892c2cad1,
  abstract     = {<p>Carbohydrate-deficient transferrin (CDT) is a glycoform profile of serum transferrin that increases in response to sustained high alcohol intake and over the last decades has become an important alcohol biomarker with clinical and forensic applications. However, the wide range of CDT measurement procedures has resulted in lack of uniform results and reference limits, and hampered comparison of results. In 2005, the IFCC therefore founded a special working group (WG) aiming for standardisation of CDT measurement. This review summarises the history of CDT and the actions taken by the WG-CDT. Initial steps included the definition of the measurand (serum disialotransferrin to total transferrin fraction expressed in %), and the determination of a well-defined anion-exchange HPLC procedure as the candidate reference measurement procedure (cRMP). Subsequent achievements were the establishment of a network of reference laboratories to perform the cRMP, setting a reference interval, and development of a reference material based on human serum for which the laboratory network assign values. Using a set of reference materials for calibration allowed for achieving equivalence of results of all present CDT measurement procedures. The final steps of the WG-CDT have been a full validation of the cRMP to make it an IFCC approved RMP, and providing guidance for international standardisation of all CDT measurement procedures.</p>},
  author       = {Helander, Anders and Wielders, Jos and Anton, Raymond and Arndt, Torsten and Bianchi, Vincenza and Deenmamode, Jean and Jeppsson, Jan-Olof and Whitfield, John B. and Weykamp, Cas and Schellenberg, François},
  issn         = {0009-8981},
  keyword      = {Alcohol biomarker,Carbohydrate-deficient transferrin,CDT,HPLC,IFCC,Serum transferrin,Standardisation},
  language     = {eng},
  month        = {08},
  pages        = {19--24},
  publisher    = {Elsevier},
  series       = {Clinica Chimica Acta},
  title        = {Standardisation and use of the alcohol biomarker carbohydrate-deficient transferrin (CDT)},
  url          = {http://dx.doi.org/10.1016/j.cca.2016.05.016},
  volume       = {459},
  year         = {2016},
}