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Label-Free Plasmonic Detection of Biomolecular Binding by a Single Gold Nanorod.

Nusz, Greg; Marinakos, Stella; Curry, Adam; Dahlin, Andreas LU ; Höök, Fredrik LU ; Wax, Adam and Chilkoti, Ashutosh (2008) In Analytical Chemistry 80(4). p.984-989
Abstract
We report the use of individual gold nanorods as plasmonic transducers to detect the binding of streptavidin to individual biotin-conjugated nanorods in real time on a surface. Label-free detection at the single-nanorod level was performed by tracking the wavelength shift of the nanorod-localized surface plasmon resonant scattering spectrum using a dark-field microspectroscopy system. The lowest streptavidin concentration that was experimentally measured was 1 nM, which is a factor of 1000-fold lower than the previously reported detection limit for streptavidin binding by biotinylated single plasmonic nanostructures. We believe that the current optical setup is able to reliably measure wavelength shifts as small as 0.3 nm. Binding of... (More)
We report the use of individual gold nanorods as plasmonic transducers to detect the binding of streptavidin to individual biotin-conjugated nanorods in real time on a surface. Label-free detection at the single-nanorod level was performed by tracking the wavelength shift of the nanorod-localized surface plasmon resonant scattering spectrum using a dark-field microspectroscopy system. The lowest streptavidin concentration that was experimentally measured was 1 nM, which is a factor of 1000-fold lower than the previously reported detection limit for streptavidin binding by biotinylated single plasmonic nanostructures. We believe that the current optical setup is able to reliably measure wavelength shifts as small as 0.3 nm. Binding of streptavidin at 1 nM concentration induces a mean resonant wavelength shift of 0.59 nm suggesting that we are currently operating at close to the limit of detection of the system. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
80
issue
4
pages
984 - 989
publisher
The American Chemical Society
external identifiers
  • pmid:18197636
  • wos:000253165400010
  • scopus:39449120055
ISSN
1520-6882
DOI
10.1021/ac7017348
language
English
LU publication?
yes
id
91e7f4f3-3927-4f3f-a0fa-8de78f53e189 (old id 1021351)
date added to LUP
2008-02-19 14:38:35
date last changed
2017-11-19 03:30:01
@article{91e7f4f3-3927-4f3f-a0fa-8de78f53e189,
  abstract     = {We report the use of individual gold nanorods as plasmonic transducers to detect the binding of streptavidin to individual biotin-conjugated nanorods in real time on a surface. Label-free detection at the single-nanorod level was performed by tracking the wavelength shift of the nanorod-localized surface plasmon resonant scattering spectrum using a dark-field microspectroscopy system. The lowest streptavidin concentration that was experimentally measured was 1 nM, which is a factor of 1000-fold lower than the previously reported detection limit for streptavidin binding by biotinylated single plasmonic nanostructures. We believe that the current optical setup is able to reliably measure wavelength shifts as small as 0.3 nm. Binding of streptavidin at 1 nM concentration induces a mean resonant wavelength shift of 0.59 nm suggesting that we are currently operating at close to the limit of detection of the system.},
  author       = {Nusz, Greg and Marinakos, Stella and Curry, Adam and Dahlin, Andreas and Höök, Fredrik and Wax, Adam and Chilkoti, Ashutosh},
  issn         = {1520-6882},
  language     = {eng},
  number       = {4},
  pages        = {984--989},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Label-Free Plasmonic Detection of Biomolecular Binding by a Single Gold Nanorod.},
  url          = {http://dx.doi.org/10.1021/ac7017348},
  volume       = {80},
  year         = {2008},
}