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Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays

Ellmark, Peter LU ; Högerkorp, Carl-Magnus LU ; Ek, Sara LU ; Beelov, Larissa; Berglund, Mattias; Rosenquist, Richard; Christopherson, Richard and Borrebaeck, Carl LU (2008) In Cancer Letters 265. p.98-106
Abstract
Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan™ antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cancer Letters
volume
265
pages
98 - 106
publisher
Elsevier
external identifiers
  • pmid:18353541
  • wos:000256730200010
  • scopus:43449096332
ISSN
1872-7980
DOI
10.1016/j.canlet.2008.02.006
language
English
LU publication?
yes
id
173039bc-1900-4f72-b0f0-acff3001dfa1 (old id 1028778)
date added to LUP
2008-02-11 10:56:34
date last changed
2017-09-17 06:03:18
@article{173039bc-1900-4f72-b0f0-acff3001dfa1,
  abstract     = {Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan™ antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.},
  author       = {Ellmark, Peter and Högerkorp, Carl-Magnus and Ek, Sara and Beelov, Larissa and Berglund, Mattias and Rosenquist, Richard and Christopherson, Richard and Borrebaeck, Carl},
  issn         = {1872-7980},
  language     = {eng},
  pages        = {98--106},
  publisher    = {Elsevier},
  series       = {Cancer Letters},
  title        = {Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays},
  url          = {http://dx.doi.org/10.1016/j.canlet.2008.02.006},
  volume       = {265},
  year         = {2008},
}