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Intracerebral grafting of neuronal cell suspensions. III. Activaty of intrastriatal nigral suspension implants as assessed by measurements of dopamine synthesis and metabolism

Schmidt, R H; Björklund, Anders LU ; Stenevi, U; Dunnett, S B and Gage, F H (1983) In Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00 Suppl. 522. p.19-28
Abstract
The activity of intrastriatal grafts of nigral cell suspensions has been monitored biochemically, using radioenzymatic assays of dopamine, its major acidic metabolite, DOPAC, and DOPA accumulation after DOPA-decarboxylase inhibition. Implants of 4-9 microliter of nigral cell suspension restored striatal DA levels by an average of 13-18%, with the highest individual values reaching about 50% of control. DOPAC was restored from about 5% in the lesioned controls to about 20% of normal in the grafted animals. The DOPAC: DA ratios and the DOPA accumulation measures indicated that the grafted DA neurons were spontaneously active and that the transmitter turnover rate was on the average some 50-100% higher than the intact intrinsic nigrostriatal... (More)
The activity of intrastriatal grafts of nigral cell suspensions has been monitored biochemically, using radioenzymatic assays of dopamine, its major acidic metabolite, DOPAC, and DOPA accumulation after DOPA-decarboxylase inhibition. Implants of 4-9 microliter of nigral cell suspension restored striatal DA levels by an average of 13-18%, with the highest individual values reaching about 50% of control. DOPAC was restored from about 5% in the lesioned controls to about 20% of normal in the grafted animals. The DOPAC: DA ratios and the DOPA accumulation measures indicated that the grafted DA neurons were spontaneously active and that the transmitter turnover rate was on the average some 50-100% higher than the intact intrinsic nigrostriatal DA neurones. These results thus provide evidence that the intrastriatal nigral suspension grafts are capable of restoring dopaminergic neurotransmission in the previously denervated striatum. (Less)
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AnimalsCorpus Striatum/surgeryDopamine/metabolism*FemaleNeurons/metabolismNeurons/transplantation*RatsRats, Inbred StrainsSubstantia Nigra/metabolismSubstantia Nigra/transplantation*Synaptic TransmissionTransplantation, Isogeneic
in
Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00
volume
Suppl. 522
pages
19 - 28
publisher
Wiley-Blackwell
language
English
LU publication?
yes
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f699dcb3-822e-4ad5-8e70-aa20210a7f37 (old id 1030560)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6326471dopt=Abstract
date added to LUP
2008-02-11 13:30:33
date last changed
2017-11-27 13:31:44
@article{f699dcb3-822e-4ad5-8e70-aa20210a7f37,
  abstract     = {The activity of intrastriatal grafts of nigral cell suspensions has been monitored biochemically, using radioenzymatic assays of dopamine, its major acidic metabolite, DOPAC, and DOPA accumulation after DOPA-decarboxylase inhibition. Implants of 4-9 microliter of nigral cell suspension restored striatal DA levels by an average of 13-18%, with the highest individual values reaching about 50% of control. DOPAC was restored from about 5% in the lesioned controls to about 20% of normal in the grafted animals. The DOPAC: DA ratios and the DOPA accumulation measures indicated that the grafted DA neurons were spontaneously active and that the transmitter turnover rate was on the average some 50-100% higher than the intact intrinsic nigrostriatal DA neurones. These results thus provide evidence that the intrastriatal nigral suspension grafts are capable of restoring dopaminergic neurotransmission in the previously denervated striatum.},
  author       = {Schmidt, R H and Björklund, Anders and Stenevi, U and Dunnett, S B and Gage, F H},
  keyword      = {AnimalsCorpus Striatum/surgeryDopamine/metabolism*FemaleNeurons/metabolismNeurons/transplantation*RatsRats,Inbred StrainsSubstantia Nigra/metabolismSubstantia Nigra/transplantation*Synaptic TransmissionTransplantation,Isogeneic},
  language     = {eng},
  pages        = {19--28},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00},
  title        = {Intracerebral grafting of neuronal cell suspensions. III. Activaty of intrastriatal nigral suspension implants as assessed by measurements of dopamine synthesis and metabolism},
  volume       = {Suppl. 522},
  year         = {1983},
}